| IED ID | IndEnz0002000154 |
| Enzyme Type ID | protease000154 |
| Protein Name |
Peptidase B EC 3.4.11.23 Aminopeptidase B |
| Gene Name | pepB YPO2889 y1342 YP_2566 |
| Organism | Yersinia pestis |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Yersiniaceae Yersinia Yersinia pseudotuberculosis complex Yersinia pestis |
| Enzyme Sequence | MTTEIMQISLSHNPADARWGEKALISTNDQGVTIHLTSHDQLGGIQRAARKIDGQGIKQVKLAGEGWGLEQSWAFWQGFRGPKGQRSVVWAELPANEKTELEQRLKIIDWVRDTINAPAEDLGPEQLAKNAIDLLCAVSCDAVSYRITKGEDLREQNYAGIYTVGRGSDRAPVLLALDYNPTGNPDAPVMACLVGKGITFDSGGYSLKQSAFMDSMKSDMGGAATLTGALALAAARGLKERVKLYLCCADNMVSGNAFKLGDIIRYRNGKTVEIMNTDAEGRLVLADGLIDASEQNAPLIIDAATLTGAAKTALGNDYHALFSFDDELAQALLNSAHSEHELFWRLPLAEFHRSQLPSNFAELNNVAGGAYSAGASTAAAFLSHFVKNYQQGWLHIDCSATYRKSAVDQWSAGATGLGVRTVANLLLAQAKQ |
| Enzyme Length | 432 |
| Uniprot Accession Number | P58475 |
| Absorption | |
| Active Site | ACT_SITE 208; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; ACT_SITE 282; /evidence=ECO:0000255|HAMAP-Rule:MF_00504 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal amino acid, Xaa, from a peptide or arylamide. Xaa is preferably Glu or Asp but may be other amino acids, including Leu, Met, His, Cys and Gln.; EC=3.4.11.23; Evidence={ECO:0000255|HAMAP-Rule:MF_00504}; |
| DNA Binding | |
| EC Number | 3.4.11.23 |
| Enzyme Function | FUNCTION: Probably plays an important role in intracellular peptide degradation. {ECO:0000255|HAMAP-Rule:MF_00504}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (18); Chain (1); Helix (16); Metal binding (7); Turn (4) |
| Keywords | 3D-structure;Aminopeptidase;Cytoplasm;Hydrolase;Manganese;Metal-binding;Protease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00504}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 6CXD; |
| Mapped Pubmed ID | 20711500; 31750992; |
| Motif | |
| Gene Encoded By | |
| Mass | 46,531 |
| Kinetics | |
| Metal Binding | METAL 196; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; METAL 201; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; METAL 201; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; METAL 219; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; METAL 278; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; METAL 280; /note=Manganese 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00504; METAL 280; /note=Manganese 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00504 |
| Rhea ID | |
| Cross Reference Brenda |