| IED ID | IndEnz0002000192 |
| Enzyme Type ID | protease000192 |
| Protein Name |
Dipeptidase EC 3.4.13.19 |
| Gene Name | pepD BL0479 |
| Organism | Bifidobacterium longum (strain NCC 2705) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Bifidobacteriales Bifidobacteriaceae Bifidobacterium Bifidobacterium longum Bifidobacterium longum (strain NCC 2705) |
| Enzyme Sequence | MACTTILVGKDASYDGSTIIARNEDSANGEFNPKRFIVVKPEEQPREYKSVISHLTITLPDDPLQYTAVPNADLKEGIWGEAGVNEANVAMSATETLTTNERVLGADPFVEYTPAKGDEPEVPGGIGEEDFLTIVLPYVKTAREGVQRLGALLEEFGTYEMNGVAFSDSNEIWWLETVGGHHWIAKRVPDEAYVTMPNQLGIDEFDLEDALGDQEAHMCSEDLAEFIETNHLDLAVENTTPFNPRDAFGSHSDSDHVYNTPRAWYMQRFLNPYDEVWDGPDADHKPTSDDIPWARQPERKVTIEDIKYVLSSHYQGTPFDPYGQLGDERTRHMYRTIGINRQSQLAVMQIRPYRPQASRAIQWMAYGSNPFNTLVPFFPNVDTTPAYLEDTTTRVTSENFYWANRIIAALCDGAFRSTSNAVERYQEKTGAMGHRLVAATDEQIARLGLTAAEEAAQSAAEEEFEADNVDGDVQPMTPDETIAALRNPEVREILAAANQTMADQLKEETEKLLDSVLYTRSMEMKNGFHMSDF |
| Enzyme Length | 533 |
| Uniprot Accession Number | Q8G6Z9 |
| Absorption | |
| Active Site | ACT_SITE 3; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=an L-aminoacyl-L-amino acid + H2O = 2 an L-alpha-amino acid; Xref=Rhea:RHEA:48940, ChEBI:CHEBI:15377, ChEBI:CHEBI:59869, ChEBI:CHEBI:77460; EC=3.4.13.19; Evidence={ECO:0000269|PubMed:17601807}; |
| DNA Binding | |
| EC Number | 3.4.13.19 |
| Enzyme Function | FUNCTION: Hydrolyzes a wide range of dipeptides. Highest activity against Ala-Gln. {ECO:0000269|PubMed:17601807}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius with Ala-Gln as substrate at pH 8.0. Activity is 30% of maximum at 20 degrees Celsius and 50% of maximum at 70 degrees Celsius. Stable when incubated for 10 minutes at temperatures of up to 70 degrees Celsius. {ECO:0000269|PubMed:17601807}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.0-9.0 with Ala-Gln as substrate at 37 degrees Celsius. Activity is very low below pH 5.5 and at pH 10.0 is about 50% of the maximum. {ECO:0000269|PubMed:17601807}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1) |
| Keywords | Dipeptidase;Direct protein sequencing;Hydrolase;Protease;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 59,751 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:48940 |
| Cross Reference Brenda |