Detail Information for IndEnz0002000339
IED ID IndEnz0002000339
Enzyme Type ID protease000339
Protein Name Xaa-Pro dipeptidase
X-Pro dipeptidase
EC 3.4.13.9
Imidodipeptidase
Proline dipeptidase
Prolidase
Gene Name pepQ PF1343
Organism Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic Lineage cellular organisms Archaea Euryarchaeota Thermococci Thermococcales Thermococcaceae Pyrococcus Pyrococcus furiosus Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Enzyme Sequence MKERLEKLVKFMDENSIDRVFIAKPVNVYYFSGTSPLGGGYIIVDGDEATLYVPELEYEMAKEESKLPVVKFKKFDEIYEILKNTETLGIEGTLSYSMVENFKEKSNVKEFKKIDDVIKDLRIIKTKEEIEIIEKACEIADKAVMAAIEEITEGKREREVAAKVEYLMKMNGAEKPAFDTIIASGHRSALPHGVASDKRIERGDLVVIDLGALYNHYNSDITRTIVVGSPNEKQREIYEIVLEAQKRAVEAAKPGMTAKELDSIAREIIKEYGYGDYFIHSLGHGVGLEIHEWPRISQYDETVLKEGMVITIEPGIYIPKLGGVRIEDTVLITENGAKRLTKTERELL
Enzyme Length 348
Uniprot Accession Number P81535
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of Xaa-|-Pro dipeptides. Also acts on aminoacyl-hydroxyproline analogs. No action on Pro-|-Pro.; EC=3.4.13.9;
DNA Binding
EC Number 3.4.13.9
Enzyme Function FUNCTION: Splits dipeptides with a prolyl in the C-terminal position and a nonpolar amino acid at the N-terminal position. {ECO:0000269|PubMed:9733678}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 100 degrees Celsius. Highly thermostable. {ECO:0000269|PubMed:9733678};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.0. {ECO:0000269|PubMed:9733678};
Pathway
nucleotide Binding
Features Beta strand (18); Chain (1); Helix (13); Metal binding (7); Mutagenesis (3); Turn (2)
Keywords 3D-structure;Cobalt;Cytoplasm;Dipeptidase;Direct protein sequencing;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (1)
Cross Reference PDB 1PV9;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 39,387
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.8 mM for Met-Pro {ECO:0000269|PubMed:9733678}; KM=3 mM for Leu-Pro {ECO:0000269|PubMed:9733678}; KM=4.2 mM for Val-Pro {ECO:0000269|PubMed:9733678}; KM=8.3 mM for Ala-Pro {ECO:0000269|PubMed:9733678}; KM=20 mM for Phe-Pro {ECO:0000269|PubMed:9733678}; Vmax=645 umol/min/mg enzyme with Met-Pro as substrate {ECO:0000269|PubMed:9733678}; Vmax=645 umol/min/mg enzyme with Leu-Pro as substrate {ECO:0000269|PubMed:9733678}; Vmax=175 umol/min/mg enzyme with Val-Pro as substrate {ECO:0000269|PubMed:9733678}; Vmax=250 umol/min/mg enzyme with Ala-Pro as substrate {ECO:0000269|PubMed:9733678}; Vmax=1000 umol/min/mg enzyme with Phe-Pro as substrate {ECO:0000269|PubMed:9733678};
Metal Binding METAL 209; /note=Cobalt 2; METAL 220; /note=Cobalt 1; METAL 220; /note=Cobalt 2; METAL 284; /note=Cobalt 1; METAL 313; /note=Cobalt 1; METAL 327; /note=Cobalt 1; METAL 327; /note=Cobalt 2
Rhea ID
Cross Reference Brenda 3.4.13.9;