| IED ID | IndEnz0002000367 |
| Enzyme Type ID | protease000367 |
| Protein Name |
Philibertain g 1 EC 3.4.22.- Philibertain g I Fragment |
| Gene Name | |
| Organism | Philibertia gilliesii (Milkweed) (Sarcostemma gilliesii) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Gentianales Apocynaceae Asclepiadoideae Asclepiadeae MOOG clade Oxypetalinae Philibertia Philibertia gilliesii (Milkweed) (Sarcostemma gilliesii) |
| Enzyme Sequence | LPASVDWRKEGAVLPIRHQGQCG |
| Enzyme Length | 23 |
| Uniprot Accession Number | P84789 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Strongly inhibited by the cysteine protease inhibitor E-64. Not inhibited by the serine protease inhibitor PMSF, the aspartic protease inhibitor pepstatin A, or by the metal ion chelator 1,10-phenanthroline. {ECO:0000269|PubMed:16328737}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Thiol protease. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH for casein is 7.6 and philibertain g 1 retains more than 80% of maximum activity between pH 6.7 and pH 8.7, and 50% of maximum activity between pH 6.1 and pH 9.8. Optimum pH for PFLNA is 6.2-7.2 and philibertain g I retains more than 80% of maximum activity between pH 5.8 and pH 7.8, and 50% of maximum activity between pH 5.2 and pH 8.6. {ECO:0000269|PubMed:16328737}; |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Disulfide bond (1); Non-terminal residue (1) |
| Keywords | Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 2,518 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.15 mM for PFLNA {ECO:0000269|PubMed:16328737}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |