| IED ID | IndEnz0002000425 |
| Enzyme Type ID | protease000425 |
| Protein Name |
Peptidase 1 EC 3.4.22.65 Allergen Der f I Major mite fecal allergen Der f 1 allergen Der f 1 |
| Gene Name | DERF1 |
| Organism | Dermatophagoides farinae (American house dust mite) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Chelicerata Arachnida Acari Acariformes Sarcoptiformes Astigmata Psoroptidia Analgoidea (feather mites) Pyroglyphidae (house-dust mites) Dermatophagoidinae Dermatophagoides Dermatophagoides farinae (American house dust mite) |
| Enzyme Sequence | MKFVLAIASLLVLSTVYARPASIKTFEEFKKAFNKNYATVEEEEVARKNFLESLKYVEANKGAINHLSDLSLDEFKNRYLMSAEAFEQLKTQFDLNAETSACRINSVNVPSELDLRSLRTVTPIRMQGGCGSCWAFSGVAATESAYLAYRNTSLDLSEQELVDCASQHGCHGDTIPRGIEYIQQNGVVEERSYPYVAREQRCRRPNSQHYGISNYCQIYPPDVKQIREALTQTHTAIAVIIGIKDLRAFQHYDGRTIIQHDNGYQPNYHAVNIVGYGSTQGDDYWIVRNSWDTTWGDSGYGYFQAGNNLMMIEQYPYVVIM |
| Enzyme Length | 321 |
| Uniprot Accession Number | P16311 |
| Absorption | |
| Active Site | ACT_SITE 133; /evidence=ECO:0000250; ACT_SITE 269; /evidence=ECO:0000250; ACT_SITE 288; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Broad endopeptidase specificity.; EC=3.4.22.65; |
| DNA Binding | |
| EC Number | 3.4.22.65 |
| Enzyme Function | FUNCTION: Thiol protease that hydrolyzes proteins, with a preference for Phe or basic residues. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (9); Chain (1); Disulfide bond (3); Glycosylation (1); Helix (7); Propeptide (1); Sequence conflict (2); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Allergen;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Secreted;Signal;Thiol protease;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 3D6S; 3RVV; 5VPK; 5VPL; |
| Mapped Pubmed ID | 22210776; |
| Motif | |
| Gene Encoded By | |
| Mass | 36,435 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.22.65; |