Detail Information for IndEnz0002000461
IED ID IndEnz0002000461
Enzyme Type ID protease000461
Protein Name Peptidase T
EC 3.4.11.4
Aminotripeptidase
Tripeptidase
Tripeptide aminopeptidase
Gene Name pepT BF1289
Organism Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / NCTC 9343 / Onslow)
Taxonomic Lineage cellular organisms Bacteria FCB group Bacteroidetes/Chlorobi group Bacteroidetes Bacteroidia Bacteroidales Bacteroidaceae Bacteroides Bacteroides fragilis Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / NCTC 9343 / Onslow)
Enzyme Sequence MNLVERFLKYVSFDTQSDELTRLTPSTPGQMVFAEYLKSELESLGLEDITLDENGYLFATLPANTEKELPVIGFIAHMDTSPDMSGKNVTPRIVEKYDGSDIVLCAEENIVLSPSQFPELLDHKGEDLIVTNGKTLLGADDKAGIAEIVSAVVYLQEHPEIKHGKIRIGFNPDEEIGEGAHKFDVQKFGCEWAYTMDGGEVGELEFENFNAAAAKITFKGRNVHPGYAKHKMINSIRIANQFITMLPRHETPEHTSGYEGFYHLIGIQGDVEQSTVSYIIRDHDRNKFEDRKKEIEHLVNKINAEFGEGTATLELRDQYYNMREKIEPVMHIIDTAFAAMEAVGVKPNVKPIRGGTDGAQLSFKGLPCPNIFAGGLNFHGRYEFVPIQNMEKAMKVIVKIAELVASK
Enzyme Length 407
Uniprot Accession Number Q5LFT7
Absorption
Active Site ACT_SITE 79; /evidence=ECO:0000255|HAMAP-Rule:MF_00550; ACT_SITE 174; /note=Proton acceptor; /evidence=ECO:0000255|HAMAP-Rule:MF_00550
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of the N-terminal residue from a tripeptide.; EC=3.4.11.4; Evidence={ECO:0000255|HAMAP-Rule:MF_00550};
DNA Binding
EC Number 3.4.11.4
Enzyme Function FUNCTION: Cleaves the N-terminal amino acid of tripeptides. {ECO:0000255|HAMAP-Rule:MF_00550}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (6)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00550}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 45,485
Kinetics
Metal Binding METAL 77; /note=Zinc 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00550; METAL 140; /note=Zinc 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00550; METAL 140; /note=Zinc 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00550; METAL 175; /note=Zinc 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00550; METAL 197; /note=Zinc 1; /evidence=ECO:0000255|HAMAP-Rule:MF_00550; METAL 379; /note=Zinc 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00550
Rhea ID
Cross Reference Brenda