Detail Information for IndEnz0002000491
IED ID IndEnz0002000491
Enzyme Type ID protease000491
Protein Name Pepsin A-3
EC 3.4.23.1
Pepsinogen-3
Gene Name PGA3
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MKWLLLLGLVALSECIMYKVPLIRKKSLRRTLSERGLLKDFLKKHNLNPARKYFPQWKAPTLVDEQPLENYLDMEYFGTIGIGTPAQDFTVVFDTGSSNLWVPSVYCSSLACTNHNRFNPEDSSTYQSTSETVSITYGTGSMTGILGYDTVQVGGISDTNQIFGLSETEPGSFLYYAPFDGILGLAYPSISSSGATPVFDNIWNQGLVSQDLFSVYLSADDQSGSVVIFGGIDSSYYTGSLNWVPVTVEGYWQITVDSITMNGEAIACAEGCQAIVDTGTSLLTGPTSPIANIQSDIGASENSDGDMVVSCSAISSLPDIVFTINGVQYPVPPSAYILQSEGSCISGFQGMNLPTESGELWILGDVFIRQYFTVFDRANNQVGLAPVA
Enzyme Length 388
Uniprot Accession Number P0DJD8
Absorption
Active Site ACT_SITE 94; /evidence="ECO:0000255|PROSITE-ProRule:PRU10094, ECO:0000269|PubMed:7663352"; ACT_SITE 277; /evidence="ECO:0000255|PROSITE-ProRule:PRU10094, ECO:0000269|PubMed:7663352"
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.; EC=3.4.23.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10094};
DNA Binding
EC Number 3.4.23.1
Enzyme Function FUNCTION: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (3); Domain (1); Propeptide (1); Sequence conflict (4); Signal peptide (1)
Keywords Aspartyl protease;Digestion;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Reference proteome;Secreted;Signal;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..15; /evidence="ECO:0000269|PubMed:2515193, ECO:0000269|PubMed:3197840"
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10580105; 1568474; 15688378; 17559360; 18844222; 19196398; 21175799; 23886209; 24895149; 25292040; 2587265; 31414587; 3366248;
Motif
Gene Encoded By
Mass 41,976
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda