| IED ID | IndEnz0002000592 |
| Enzyme Type ID | protease000592 |
| Protein Name |
Lysosomal Pro-X carboxypeptidase EC 3.4.16.2 Proline carboxypeptidase Prolylcarboxypeptidase PRCP |
| Gene Name | Prcp |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MGCRALLLLSFLLLGAATTIPPRLKTLGSPHLSASPTPDPAVARKYSVLYFEQKVDHFGFADMRTFKQRYLVADKHWQRNGGSILFYTGNEGDIVWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFGQDSFKDSQHLNFLTSEQALADFAELIRHLEKTIPGAQGQPVIAIGGSYGGMLAAWFRMKYPHIVVGALAASAPIWQLDGMVPCGEFMKIVTNDFRKSGPYCSESIRKSWNVIDKLSGSGSGLQSLTNILHLCSPLTSEKIPTLKGWIAETWVNLAMVNYPYACNFLQPLPAWPIKEVCQYLKNPNVSDTVLLQNIFQALSVYYNYSGQAACLNISQTTTSSLGSMGWSFQACTEMVMPFCTNGIDDMFEPFLWDLEKYSNDCFNQWGVKPRPHWMTTMYGGKNISSHSNIIFSNGELDPWSGGGVTRDITDTLVAINIHDGAHHLDLRAHNAFDPSSVLLSRLLEVKHMKKWILDFYSNIQ |
| Enzyme Length | 491 |
| Uniprot Accession Number | Q7TMR0 |
| Absorption | |
| Active Site | ACT_SITE 177; /note=Charge relay system; /evidence=ECO:0000255; ACT_SITE 428; /note=Charge relay system; /evidence=ECO:0000255; ACT_SITE 453; /note=Charge relay system; /evidence=ECO:0000255 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Cleavage of a -Pro-|-Xaa bond to release a C-terminal amino acid.; EC=3.4.16.2; |
| DNA Binding | |
| EC Number | 3.4.16.2 |
| Enzyme Function | FUNCTION: Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (1); Chain (1); Disulfide bond (4); Glycosylation (5); Propeptide (1); Region (1); Sequence conflict (4); Signal peptide (1) |
| Keywords | Alternative splicing;Carboxypeptidase;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Protease;Reference proteome;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lysosome {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11217851; 12466851; 12520002; 12904583; 14610273; 19620781; 21267068; 21297000; 21677750; 22079761; 22202165; 22454290; 23392115; 23744584; 24161824; 27437782; 27480124; 28160049; 29459251; 29514215; 31786979; 7490078; |
| Motif | |
| Gene Encoded By | |
| Mass | 55,027 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.16.2; |