| IED ID | IndEnz0002000593 |
| Enzyme Type ID | protease000593 |
| Protein Name |
Pepsin A EC 3.4.23.1 |
| Gene Name | PGA |
| Organism | Gallus gallus (Chicken) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Sauropsida Sauria (diapsids) Archelosauria Archosauria Dinosauria Saurischia Theropoda Coelurosauria Aves Neognathae Galloanserae Galliformes Phasianidae (turkeys) Phasianinae Gallus Gallus gallus (Chicken) |
| Enzyme Sequence | SIHRVPLKKGKSLRKQLKDHGLLEDFLKKHPYNPASKYHPVLTATESYEPMTNYMDASYYGTISIGTPQQDFSVIFDTGSSNLWVPSIYCKSSACSNHKRFDPSKSSTYVSTNETVYIAYGTGSMSGILGYDTVAVSSIDVQNQIFGLSETEPGSFFYYCNFDGILGLAFPSISSSGATPVFDNMMSQHLVAQDLFSVYLSKDGETGSFVLFGGIDPNYTTKGIYWVPLSAETYWQITMDRVTVGNKYVACFFTCQAIVDTGTSLLVMPQGAYNRIIKDLGVSSDGEISCDDISKLPDVTFHINGHAFTLPASAYVLNEDGSCMLGFENMGTPTELGEQWILGDVFIREYYVIFDRANNKVGLSPLS |
| Enzyme Length | 367 |
| Uniprot Accession Number | P00793 |
| Absorption | |
| Active Site | ACT_SITE 77; ACT_SITE 260 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.; EC=3.4.23.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10094}; |
| DNA Binding | |
| EC Number | 3.4.23.1 |
| Enzyme Function | FUNCTION: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (3); Domain (1); Glycosylation (1); Propeptide (1) |
| Keywords | Aspartyl protease;Digestion;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 40,432 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |