| IED ID | IndEnz0002000608 |
| Enzyme Type ID | protease000608 |
| Protein Name |
Aspartic protease pepA EC 3.4.23.- |
| Gene Name | Lema_P089170.1 |
| Organism | Leptosphaeria maculans (strain JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8) (Blackleg fungus) (Phoma lingam) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta dothideomyceta Dothideomycetes Pleosporomycetidae Pleosporales Pleosporineae Leptosphaeriaceae Leptosphaeria Leptosphaeria maculans species complex Leptosphaeria maculans (Blackleg fungus) (Phoma lingam) Leptosphaeria maculans 'brassicae' group Leptosphaeria maculans (strain JN3 / isolate v23.1.3 / race Av1-4-5-6-7-8) (Blackleg fungus) (Phoma lingam) |
| Enzyme Sequence | MPSIVSLTAALTFVGAVIASPVEKRSAFSVEQVPHTTYLKNGPAQKVKTLRKYGKPVPQSLLDAAESRDAVGETFTASAVGDGSDPAVPSDQYDSSYLSPVTVGSTTVELDFDTGSADLWVFSSLQASSQLSGHQYYQADASKLKSGYSWKISYGDGSGASGKVYADKVVVGGVTATSQAVEAATSVSAQFSRDSNTDGLLGLAFSSINTVSPEPQKTFFDTVKPQLAEPLFAVNLKYHAAGTYDFGYIDKSKYTGPITYVNVDDSQGFWGISATAYGVGGTTTQRAISGILDTGTTLVYTDTAIVKAYYAKVSGAKLDNIQGGYVFPCSATLPNFSITVGGVAQVVPGKHINYSPVDSSGTTCFGGIQTNDGLGMSIFGDIFLKSKYVVHEAAPDSPPRIGLAQSASV |
| Enzyme Length | 409 |
| Uniprot Accession Number | E5A7T3 |
| Absorption | |
| Active Site | ACT_SITE 113; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103; ACT_SITE 293; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: Secreted aspartic endopeptidase that allows assimilation of proteinaceous substrates. The scissile peptide bond is attacked by a nucleophilic water molecule activated by two aspartic residues in the active site. Shows a broad primary substrate specificity. Favors hydrophobic residues at the P1 and P1' positions. {ECO:0000250|UniProtKB:Q12567}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (1); Domain (1); Erroneous initiation (1); Glycosylation (1); Propeptide (1); Signal peptide (1) |
| Keywords | Aspartyl protease;Disulfide bond;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q12567}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 42,721 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |