| IED ID | IndEnz0002000780 |
| Enzyme Type ID | protease000780 |
| Protein Name |
Proprotein convertase subtilisin/kexin type 4 EC 3.4.21.- KEX2-like endoprotease 3 Neuroendocrine convertase 3 NEC 3 Prohormone convertase 3 |
| Gene Name | Pcsk4 Nec-3 Nec3 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MRPSQTELWLGLTLTLALLAVRWASAQAPIYVSSWAVRVTKGYQEAERLARKFGFVNLGQIFPDDQYFHLRHRGVAQQSLTPHWGHRLRLKKDPKVRWFEQQTLRRRVKRSLVVPTDPWFSKQWYMNKEIQQDLNILKAWNQGLTGRGVVISILDDGIEKDHPDLWANYDPLASYDFNDYDPDPQPRYTPNDENRHGTRCAGEVSATANNGFCGAGVAFNARIGGVRMLDGAITDIVEAQSLSLQPQHIHIYSASWGPEDDGRTVDGPGLLTQEAFRRGVTKGRQGLGTLFIWASGNGGLHYDNCNCDGYTNSIHTLSVGSTTRQGRVPWYSEACASTFTTTFSSGVVTDPQIVTTDLHHQCTDKHTGTSASAPLAAGMIALALEANPLLTWRDLQHLVVRASRPAQLQAEDWRINGVGRQVSHHYGYGLLDAGLLVDLARVWLPTKPQKKCAIRVVHTPTPILPRMLVPKNVTACSDGSRRRLIRSLEHVQVQLSLSYSRRGDLEIFLTSPMGTRSTLVAIRPLDISGQGYNNWIFMSTHYWDEDPQGLWTLGLENKGYYFNTGTLYYYTLLLYGTAEDMTARPQAPQVTSRARACVQRDTEGLCQESHSPLSILAGLCLISSQQWWWLYSHPQQPVTEGQASCHPPVTPAAAA |
| Enzyme Length | 655 |
| Uniprot Accession Number | P29121 |
| Absorption | |
| Active Site | ACT_SITE 155; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 196; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 370; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Proprotein convertase involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. In males, important for ADAM2 processing as well as other acrosomal proteins with roles in fertilization and critical for normal fertilization events such as sperm capacitation, acrosome reaction and binding of sperm to zona pellucida (PubMed:9192653, PubMed:16371590, PubMed:19342015, PubMed:21302280). Plays also a role in female fertility, involved in the regulation of trophoblast migration and placental development, may be through the proteolytical processing and activation of proteins such as IGF2 (By similarity). May also participate in folliculogenesis in the ovaries (PubMed:11164898). {ECO:0000250|UniProtKB:Q6UW60, ECO:0000269|PubMed:11164898, ECO:0000269|PubMed:16371590, ECO:0000269|PubMed:19342015, ECO:0000269|PubMed:21302280, ECO:0000269|PubMed:9192653}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (7); Chain (1); Domain (2); Glycosylation (1); Propeptide (1); Sequence conflict (2); Signal peptide (1) |
| Keywords | Alternative splicing;Cleavage on pair of basic residues;Cytoplasmic vesicle;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome membrane {ECO:0000269|PubMed:16371590, ECO:0000269|PubMed:21080038, ECO:0000269|PubMed:21302280}. |
| Modified Residue | |
| Post Translational Modification | PTM: N-glycosylated. {ECO:0000250|UniProtKB:Q6UW60}.; PTM: Synthesized in the endoplasmic reticulum as a zymogen, is matured by autocatalytic cleavage between the prodomain and the catalytic domain. {ECO:0000250|UniProtKB:Q6UW60}. |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000250 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10341094; 10701998; 10984455; 11217851; 1429684; 15226823; 16141072; 16163737; 20059953; 21267068; 21677750; 22827436; 24194600; 27626380; 30590051; 7782070; 7819324; 8422932; 8698813; 9885250; |
| Motif | |
| Gene Encoded By | |
| Mass | 73,214 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.4.21.B24;3.4.21.B25; |