| IED ID | IndEnz0002000838 |
| Enzyme Type ID | protease000838 |
| Protein Name |
Pyrrolidone-carboxylate peptidase EC 3.4.19.3 5-oxoprolyl-peptidase Pyroglutamyl-peptidase I PGP-I Pyrase |
| Gene Name | pcp |
| Organism | Bacillus amyloliquefaciens (Bacillus velezensis) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus amyloliquefaciens group Bacillus amyloliquefaciens (Bacillus velezensis) |
| Enzyme Sequence | MEKKVLLTGFDPFGGETVNPSWEAVKRLNGAAEGPASIVSEQVPTVFYKSLAVLREAIKKHQPDIIICVGQAGGRMQITPERVAINLNEARIPDNEGNQPVGEDISQGGPAAYWTGLPIKRIVEEIKKEGIPAAVSYTAGTFVCNHLFYGLMDEISRHHPHIRGGFIHIPYIPEQTLQKSAPSLSLDHITKALKIAAVTAAVHEDDIETGGGELH |
| Enzyme Length | 215 |
| Uniprot Accession Number | P46107 |
| Absorption | |
| Active Site | ACT_SITE 81; ACT_SITE 144; ACT_SITE 168 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.; EC=3.4.19.3; |
| DNA Binding | |
| EC Number | 3.4.19.3 |
| Enzyme Function | FUNCTION: Removes 5-oxoproline from various penultimate amino acid residues except L-proline. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.5. Stable from pH 7.0 to 9.0.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (7); Chain (1); Helix (6); Mutagenesis (2); Turn (1) |
| Keywords | 3D-structure;Cytoplasm;Hydrolase;Protease;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (3) |
| Cross Reference PDB | 1AUG; 3RNZ; 3RO0; |
| Mapped Pubmed ID | 21875148; |
| Motif | |
| Gene Encoded By | |
| Mass | 23,287 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |