| IED ID | IndEnz0002000859 |
| Enzyme Type ID | protease000859 |
| Protein Name |
Protein disulfide isomerase-like 1-1 AtPDIL1-1 EC 5.3.4.1 Protein disulfide-isomerase 1 PDI 1 Protein disulfide-isomerase 5 AtPDI5 |
| Gene Name | PDIL1-1 PDI5 At1g21750 F8K7.19 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAMRGFTLFSILVLSLCASSIRSEETETKEFVLTLDHTNFTDTINKHDFIVVEFYAPWCGHCKQLAPEYEKAASALSSNVPPVVLAKIDASEETNREFATQYEVQGFPTIKIFRNGGKAVQEYNGPREAEGIVTYLKKQSGPASAEIKSADDASEVVSDKKVVVVGIFPKLSGSEFDSFMAIAEKLRSELDFAHTSDAKLLPRGESSVTGPVVRLFKPFDEQFVDSKDFDGEALEKFVKESSIPLITVFDKDPNNHPYVIKFFESTNTKAMLFINFTGEGAESLKSKYREVATSNKGQGLSFLLGDAENSQGAFQYFGLEESQVPLIIIQTADDKKYLKTNVEVDQIESWVKDFKDGKIAPHKKSQPIPAENNEPVKVVVSDSLDDIVLNSGKNVLLEFYAPWCGHCQKLAPILDEVAVSYQSDSSVVIAKLDATANDFPKDTFDVKGFPTIYFKSASGNVVVYEGDRTKEDFISFVDKNKDTVGEPKKEEETTEEVKDEL |
| Enzyme Length | 501 |
| Uniprot Accession Number | Q9XI01 |
| Absorption | |
| Active Site | ACT_SITE 59; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 62; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 404; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 407; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Catalyzes the rearrangement of -S-S- bonds in proteins.; EC=5.3.4.1; |
| DNA Binding | |
| EC Number | 5.3.4.1 |
| Enzyme Function | FUNCTION: Protein disulfide isomerase that associates with RD21A protease for trafficking from the ER through the Golgi to lytic and protein storage vacuoles of endothelial cells in developing seeds. Regulates the timing of programmed cell death (PCD) of the endothelial cells by chaperoning and inhibiting cysteine proteases during their trafficking to vacuoles. {ECO:0000269|PubMed:18676877}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Chain (1); Disulfide bond (2); Domain (2); Glycosylation (2); Motif (1); Signal peptide (1); Site (6) |
| Keywords | Alternative splicing;Disulfide bond;Endoplasmic reticulum;Glycoprotein;Isomerase;Redox-active center;Reference proteome;Repeat;Signal;Vacuole |
| Interact With | P43297; Q9FMH8 |
| Induction | INDUCTION: By chemically-induced ER stress response. {ECO:0000269|PubMed:18574595}. |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000255|PROSITE-ProRule:PRU10138, ECO:0000269|PubMed:18676877}. Vacuole {ECO:0000269|PubMed:18676877}. Note=Found in protein storage vacuoles and lytic vacuoles in endothelial cells of developing seeds. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..23; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11826309; 12169696; 12492832; 12566592; 12805589; 12853141; 15028209; 15282545; 15352244; 15539469; 15618420; 15894741; 15908592; 15978049; 15998247; 16199612; 16242667; 16247729; 16531467; 16547123; 16618929; 16648214; 16844841; 16979780; 17153927; 17163439; 17194767; 17432890; 17526915; 17828791; 17916636; 18156219; 18650403; 18775970; 18931141; 19880396; 20118269; 20405473; 21909944; 24064926; 25804820; 26240315; 26300531; 27247031; 28627464; |
| Motif | MOTIF 498..501; /note=Prevents secretion from ER; /evidence=ECO:0000255|PROSITE-ProRule:PRU10138 |
| Gene Encoded By | |
| Mass | 55,602 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |