| IED ID | IndEnz0002000861 |
| Enzyme Type ID | protease000861 |
| Protein Name |
RNA1 polyprotein Genome polyprotein B P1 Cleaved into: Picornain 3C-like protease 3C-like protease EC 3.4.22.- 24 kDa protein ; RNA-directed RNA polymerase EC 2.7.7.48 87 kDa protein Fragment |
| Gene Name | |
| Organism | Andean potato mottle virus (APMV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Secoviridae Comovirinae Comovirus Andean potato mottle virus (APMV) |
| Enzyme Sequence | GIHVAGGRGKGYACLMPPLRPKAQAQSAQEHFEIFPYEQETNAGLALVGELKQGVYVSCPTKTSFERTPESYHLGLPCEKEPSILSSHDPRIPEHVEGYCPFRAGIQKYANPMGHLDHDLMYEVAHDMQESWHDCVQDFTFPEVDLETAINGIDMVEYMECIPKSTSEGFPHVLSRAPGEKGKMRFLEGDGEKFSLREGTSVKKAYDLLQEEIDRSVPTLVAIECPKDEKLPLRKIYTSPKTRCFSILPMEYNLLVRQKFLHFVRFMMKRRDVLPSQVGVNPYSLEWGAIARRLQEVGNSILCCDYSSFDGLMSSQVMSCIADTMNDFMGGDVSLKRQRKNLLMACCSRFSVVKGNVWRVEGGIPSGFPLTVVMNGIFNELLVRYCFKKIMREGGATPLECSAFDSYIRFVVYGDDNLISVSPVIHDKFNGKLLKECMARFGVTITDGKDKTLPTLEFRPLEDCDFLKRGFIQRSELVWDAPEERSSLYTQLHYVSTKMQSLEDAYTGNLVNVIRELYMHSPKEASDLRRKALRDLPWLSRSKIGTMENVQAFYAMQRAGYRMDESIDVICDLAKLGKYVKGEACKEIVWLTPTVGACDLRYFDWQNAKVDEFWVLCQTNYHEFDENRVMQLCWTPGSGRGGLPTAHWLRTCMLLEKGNVRKKLHWAMAEKKKIIFCAKGGVLIPTVMAGIFLSKEDPMLNLAGVSTLTCAMESVKTLGFLKEGNLNLF |
| Enzyme Length | 729 |
| Uniprot Accession Number | Q02941 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 3.4.22.-; 2.7.7.48 |
| Enzyme Function | FUNCTION: [Picornain 3C-like protease]: Thiol protease that cleaves the RNA1 and RNA2 polyproteins. {ECO:0000250|UniProtKB:P03600}.; FUNCTION: [RNA-directed RNA polymerase]: Replicates the viral genome. {ECO:0000250|UniProtKB:P03600}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (3); Domain (2); Non-terminal residue (1); Site (1) |
| Keywords | Covalent protein-RNA linkage;Host endoplasmic reticulum;Hydrolase;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Thiol protease;Transferase;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [RNA-directed RNA polymerase]: Host endoplasmic reticulum {ECO:0000250|UniProtKB:P03600}. |
| Modified Residue | |
| Post Translational Modification | PTM: [RNA1 polyprotein]: Specific enzymatic cleavages by picornain 3C-like protease in vivo yield mature proteins. Picornain 3C-like protease is autocatalytically processed. {ECO:0000250|UniProtKB:P03600}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 82,621 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |