| IED ID | IndEnz0002000871 |
| Enzyme Type ID | protease000871 |
| Protein Name |
RNA1 polyprotein Genome polyprotein B P1 Cleaved into: Protease cofactor; Putative helicase EC 3.6.4.- Membrane-binding protein NTP-binding protein NTB ; Viral genome-linked protein VPg ; Picornain 3C-like protease 3C-like protease EC 3.4.22.- ; RNA-directed RNA polymerase EC 2.7.7.48 |
| Gene Name | |
| Organism | Cherry rasp leaf virus (isolate Potato/United States) (CRLV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Secoviridae Cheravirus Cherry rasp leaf virus (CRLV) Cherry rasp leaf virus (isolate Potato/United States) (CRLV) |
| Enzyme Sequence | MGIFTLCSCGVPCPTRSFYRRHMASGCDLLPTRQQLADVGYTEPTVIAEVVTPPTQAPLELFVEISPPVLAPFEPSVEISLPIQAPAEPIVEASPPAATQFVGIEALIASGPCFFGSFGPFEEYYSSPVGPLTRFDTLRREGHCAARARVAAVAAKAVIVQQTLSETAAAMRATLPLWMKGQVAPPVKSKRALKREAKAKAKADYLASDEAYYKATDGLTALPPGVSRDVHMRQLDAMEVAYLAHVGDVAARGFQRRQVLRAAYKARCEKRAFKRFLEEVDFLCLPVHIVDKIQTPFDGEQAAAPEMQKGMVYATSRRQVKTRRSFSSFLPKEDFSFTLGVCPARSPTVTPSSTPSTSRSSSPEPRVSSPSGVLPEKAACIGFCSHGSECTEHFCFGYLNLKSEALASQYLAWLLQTKLPGGISCFELEVSSYLEQCQDTMEAIDLWWHAMDRYCFNFKSSKYVILDNFLCKHSIAKDQTPREFLAKHRIAKAKALHRVPSRKEKMQALCEQRADKAWDAYIELNKNCKVGEGPLEYLKAAKDRCVEFFSPFTKYCNEAIRSLNPLVAILGPFKDGFWTCFNNLREKCLKMVNDHWLAFAAGTTLVLSLIFLLCIICLVKIFSILIANLGLGVVAITTLVTALVVGFFLFNGMLEQAADLQLCSLVASDFLNFLAQNQGSALVAGTMASVQDDLRGQGVSWCFSALYKLISRVVPVGIKETSILFNSIGNVSRSANHSKDFFLNMKEMACSWMDALSDAMALISDDSVSAMQVLKHLCEHDFLDWAKKVERYAGETYDSLIISPAERLKILRILADQQESFQKAFYNPRIASKAPRLMLTEFNRLSTLLRDAHNALSRASLFDRQRTPPFWVHLYSENGGTGKSMAMMPLGNHMLDAIGEPKTCRFVTRNVASKFLNGYQHQPCFLMDEFGAAPKQDYSDEVTMLDLVSPNALTLNMAAIGEKNTMFTSKLIISTANRRLAHPDVKLGANLDGFLRRRNILAEVVLVSGKPHFHEFNLLAPRTEQKVYLNRAMQESQVPDPLTPQEFYSLCTENFVNFLNQQSSTVAMSAGLNYVRSSDFSHLKDFLLFKIGLDFEENEVERIVTDYGNSLKNETIFPPEHEQIFQKWKDALDSLTLPELVSLLDKSVSETFVYTLISENHPNVVMSSLTPYECMIYAICKKKYREGTEAKLPFPEGETTSYGASFLSFVAQVALCIPKWALFCVALCAVLLVGYLIIKFAIFLFSGVVTLLGALAFSNLSGDGAEDSPSFDTNRKRGGVKFEYSTKWDASSTNRFAESYSENGTIPAGTSWADFFGEGPEEEPSQSLLNLLKHQVVLIAEPTKVVYNCIALGGRNFLITKHVWDLMPACNYGLYGYAVAKDRIFISPRIRPCAQLKGRDLVIVQLPDSVPPFTSLPRDIFLENMAKAPKTANACLVVAKPLFERRSVAKLEQTIYPFKQLPQVHSKDTYSCGSLGSKQMPACYSYVFETYAGLCTSPLIAQEGGRCIILGLHVVGDRSKMGYAQIVTLDDFSDVALSDKVGQGPEEMYIPTKNSECFGSVTKLGAWTGPKPYFLEKTSLIPSLISTSIDVERTTEPAILSQRDKRLKDSINPEFDVFLEGMKKYAVEAHSLDEDLEVFEDALDRVFLEIPEHACEDLTNDQVCNGIEDDPYAEGIVMQTAEGFPFCTQRPAGASGKSWLFAGAPGDWHIVPGSLLANEMHKKEVAPSRGLFEPLIGIDFPKDEKVDSSKVYIKPKTRLFTILPVDYNILVRKYFLSSVSHIMTQHNTIPVKVGIDCLSNEWSILYHQLRSKGTNWFNGDYSRFDGITPRNVLQGIVKRINKFYNNKNSLAITDSNLSINSDLARSLLTDMASTRYGLTNGDLWYVTSGIPSGFPLTVIVNSLVNNFFIHFSYIKLMKREELNSLYPLHSFRQMVAYATYGDDNLVSVNDVITEKFNLVKIADLLAEHGVTLKNGADKNEEILSPFYPLEKVDFLKRKFVHYQGHVVAPLNPVNITERLHWIRKGLGEADATLENCSSAAFEALFHGRCYYDTLVAKIYKACAASKLSIQLPTYNDALAIFLSNDSFAKAIQTISLDLPKAIFVNKSNYFVSEIFPDVFFCSNERNVTLHKLLEITTTRNICYISRNYESRNSSRGLFSLKGEGWALAPVSARLVVYKNMQKPVYFVDEANDGLALAYCLDYMLRIKGVSRSRLAQVLYNIFGHDETLCSRIASNFSLLDSNKYMPPHKK |
| Enzyme Length | 2250 |
| Uniprot Accession Number | Q6EWG9 |
| Absorption | |
| Active Site | ACT_SITE 1362; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 1400; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 1495; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 3.6.4.-; 3.4.22.-; 2.7.7.48 |
| Enzyme Function | FUNCTION: Picornain 3C-like protease is a thiol protease that probably cleaves the B and M polyproteins.; FUNCTION: Viral genome-linked protein (VPg) plays a role in RNA replication. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (5); Compositional bias (1); Domain (3); Modified residue (1); Region (1); Site (4); Transmembrane (1) |
| Keywords | ATP-binding;Covalent protein-RNA linkage;Helicase;Host membrane;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;Phosphoprotein;Protease;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Putative helicase]: Host membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. |
| Modified Residue | MOD_RES 1299; /note=O-(5'-phospho-RNA)-serine; /evidence=ECO:0000250 |
| Post Translational Modification | PTM: Specific enzymatic cleavages by picornain 3C-like protease in vivo yield mature proteins. Picornain 3C-like protease is autocatalytically processed (By similarity). {ECO:0000250}.; PTM: Viral genome-linked protein (VPg) is uridylylated by the polymerase and is covalently linked to the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase (By similarity). {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 251,009 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |