Detail Information for IndEnz0002000876
IED ID IndEnz0002000876
Enzyme Type ID protease000876
Protein Name RNA1 polyprotein
P1

Cleaved into: Putative helicase
EC 3.6.4.-
Putative NTP-binding protein
; Probable picornain 3C-like protease
3C-like protease
EC 3.4.22.-
; Probable RNA-directed RNA polymerase
EC 2.7.7.48
Gene Name
Organism Satsuma dwarf virus (isolate Satsuma mandarin/Japan/S-58/1977) (SDV)
Taxonomic Lineage Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Secoviridae Sadwavirus Satsumavirus Satsuma dwarf virus (SDV) Satsuma dwarf virus (isolate Satsuma mandarin/Japan/S-58/1977) (SDV)
Enzyme Sequence MEIYGFSPLSFQDTESWKNSAFAACRGCKTYPIEEVGTDGSVRIRELPVGLVLKGVMAQYRSILWTQFVADPATTTVDRKSFDFWLFCRRAEAAQERAFRARQSKRAAKAAALDAEGLIDRSYKGRTYKVSTRGLSWKSVRAAQKAARNAAKNFGFAISANPFSALSALPSEGKGLSAGETPATVAEVAPQCFWAALRSLKREFSETPVEDLPFLGLVLPALPQRNGELARTLRAQLRAHQAARVVASGVSLREIPRRTTTTVKVVVPRVTIPVVTFNGAVLHHPKHPVIVALLKRVTIRAKPICERRTYVRLVDGPGLFPSPRTPTLDLGFMEKVSELATMPGDSIEKCIAIKCILSNDYNFKEDADMGDVLLSLQSKYTGGGDTAEHRGFISGALYGAGVCVVTSAWKFLDATLHASQIGTQISQILDYIQSGLAWLSSSYASIANFFTKGKVYIMECLELVKAKLPSYLFSAEVGRYILLLLGVFLCLGLVNSLIYSVAPQYSLSFGTVCKISLGALALVGLGDLVAYLFNSPGAKLRAFVNIVCNMCGCKNFFAPSSDGEERSGFSVLSMMGAICALQSLLPANVSRFSWDCGKWAQTFKTGFDCHEKFATVCESLSVWLLSKVGLFKSSESQAMQTILLSSGINTCGWLEKVAAFHSDVHSSQICIPDLLLRARQLIETGDTISDLLSNSTVSLSFLLRERIKLALRELKEDHTQLQLAVDVSVSSECPFVLFFAGDSGVGKSTAMKKFREEVLDKLGYPKTARFYPRNPGEKFWSGYLRHTAVIYDDFAQIPQGDMTYDEAELIRIVTNAVVTVPMAIAEEKGRTFRSKFVFACTNRYCESEDAPLADEMAFRRRRHLYIHVARKPGVEPGPRGVDNLEFSEMDNRDSNGEPAYQLNDQGKRVLINQKLTYDQLLQLYFERYQAFKVLEGQLEGSVARAPSSSNYEGCDNWWTLVGSEAKFEAVYFNGEPVSEDDQLTQLGNYKTRYDILRARVILESIDYADCDFIIKNFSAMGEGIYHADPEVNATGQRHFSAMSPVTRRLICSCLKTRREDEIKSHSLLSKFKSLLTVPVDAWKAAPAWFKCISLLLVAGGVGYALCRAISGIIGIFRKGPAAASLALFSGGIGAVLRGDSPDDPREERDNPDVIVTKGRGKAIWAGAEIPEALEALRKSQVVLMGTGRKGEPVMCSALPITSHSVLCTTHEIEAFDPNQNMSLIVNNSIYSFVLIPGAIMYKRYDQPNLVDKVHELVRVDLPKNKGFSLNARAQFSEDFYDNGTAMKCWVVPNKNPAVADIMRKVDCEKSSQIIDIFASELSRSSGCEPVRQTQRFIYADGPALNGHCGRLLCANLAGHWRVIGMCAGEGKNRAGVTKALYADIPHEFLRADNLNAVQRGAELDAAILDRFSIPISECRKELTPMTTRLGYVVGQYPRALRKTSIVPSIIHDNLWRKPETEPTILGKIDDRSPFPYDPYATIGEKFVQEVGPIDLSVGSDASLVVANIGSSWKAVGKPQCPTVLTWEVAINGDAAIPYCERLPLSTSEGYPDSIQRNFGEKGKKRFFDLKGENVRVPTPALMEELEVLERELQKEEVCLTCINTACAKDEKTAPKKVRVQPKTRIFEILPFQINIIIRRYLMFWMQLLMVAHDELPSKVGINVYSESWDRLLGRHTRLANHFTGDYSGFDTSTPRVLVYAIIDKINELADDGEVNQRTRRNIIRFVLNRYLISDGVLYEIHGGTPSGFAPTVMINSVVNEFYLKWSWIGLLKEAGYANQATLYAFHEATEISLYGDDNFVSVATPVASVYNLTTISNFLGRIGVKLGDGAKTGTIKPFIPLEEVDFLKRQFVADSGSTAILCPLKKISIEERLFYVRGGQDEIAALELNIATALCEAFFHGKEYFSFLEGKIIEAMRKSGVALSRPLPTMESVRAWYMSQRGNTKIRSPSFEGLGTMSGILNIGLAEARSVGGVACFSGIEFRGRSDDHLMVIPTYIPGGWRTKQQQTYISFVRDSEKMAQVIKRVAHFSTVVATDKSMAYLVAICIAYSRGSISRMEVRCHVQNLKVAEMLLCNQICNFL
Enzyme Length 2081
Uniprot Accession Number Q9WAL8
Absorption
Active Site ACT_SITE 1210; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 1255; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 1348; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
DNA Binding
EC Number 3.6.4.-; 3.4.22.-; 2.7.7.48
Enzyme Function FUNCTION: Picornain 3C-like protease is a thiol protease that probably cleaves the polyprotein. {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding NP_BIND 741..748; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00551
Features Active site (3); Chain (4); Domain (3); Nucleotide binding (1); Region (1); Transmembrane (2)
Keywords ATP-binding;DNA replication;Host membrane;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 230,447
Kinetics
Metal Binding
Rhea ID RHEA:21248
Cross Reference Brenda