| IED ID | IndEnz0002000876 |
| Enzyme Type ID | protease000876 |
| Protein Name |
RNA1 polyprotein P1 Cleaved into: Putative helicase EC 3.6.4.- Putative NTP-binding protein ; Probable picornain 3C-like protease 3C-like protease EC 3.4.22.- ; Probable RNA-directed RNA polymerase EC 2.7.7.48 |
| Gene Name | |
| Organism | Satsuma dwarf virus (isolate Satsuma mandarin/Japan/S-58/1977) (SDV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Secoviridae Sadwavirus Satsumavirus Satsuma dwarf virus (SDV) Satsuma dwarf virus (isolate Satsuma mandarin/Japan/S-58/1977) (SDV) |
| Enzyme Sequence | MEIYGFSPLSFQDTESWKNSAFAACRGCKTYPIEEVGTDGSVRIRELPVGLVLKGVMAQYRSILWTQFVADPATTTVDRKSFDFWLFCRRAEAAQERAFRARQSKRAAKAAALDAEGLIDRSYKGRTYKVSTRGLSWKSVRAAQKAARNAAKNFGFAISANPFSALSALPSEGKGLSAGETPATVAEVAPQCFWAALRSLKREFSETPVEDLPFLGLVLPALPQRNGELARTLRAQLRAHQAARVVASGVSLREIPRRTTTTVKVVVPRVTIPVVTFNGAVLHHPKHPVIVALLKRVTIRAKPICERRTYVRLVDGPGLFPSPRTPTLDLGFMEKVSELATMPGDSIEKCIAIKCILSNDYNFKEDADMGDVLLSLQSKYTGGGDTAEHRGFISGALYGAGVCVVTSAWKFLDATLHASQIGTQISQILDYIQSGLAWLSSSYASIANFFTKGKVYIMECLELVKAKLPSYLFSAEVGRYILLLLGVFLCLGLVNSLIYSVAPQYSLSFGTVCKISLGALALVGLGDLVAYLFNSPGAKLRAFVNIVCNMCGCKNFFAPSSDGEERSGFSVLSMMGAICALQSLLPANVSRFSWDCGKWAQTFKTGFDCHEKFATVCESLSVWLLSKVGLFKSSESQAMQTILLSSGINTCGWLEKVAAFHSDVHSSQICIPDLLLRARQLIETGDTISDLLSNSTVSLSFLLRERIKLALRELKEDHTQLQLAVDVSVSSECPFVLFFAGDSGVGKSTAMKKFREEVLDKLGYPKTARFYPRNPGEKFWSGYLRHTAVIYDDFAQIPQGDMTYDEAELIRIVTNAVVTVPMAIAEEKGRTFRSKFVFACTNRYCESEDAPLADEMAFRRRRHLYIHVARKPGVEPGPRGVDNLEFSEMDNRDSNGEPAYQLNDQGKRVLINQKLTYDQLLQLYFERYQAFKVLEGQLEGSVARAPSSSNYEGCDNWWTLVGSEAKFEAVYFNGEPVSEDDQLTQLGNYKTRYDILRARVILESIDYADCDFIIKNFSAMGEGIYHADPEVNATGQRHFSAMSPVTRRLICSCLKTRREDEIKSHSLLSKFKSLLTVPVDAWKAAPAWFKCISLLLVAGGVGYALCRAISGIIGIFRKGPAAASLALFSGGIGAVLRGDSPDDPREERDNPDVIVTKGRGKAIWAGAEIPEALEALRKSQVVLMGTGRKGEPVMCSALPITSHSVLCTTHEIEAFDPNQNMSLIVNNSIYSFVLIPGAIMYKRYDQPNLVDKVHELVRVDLPKNKGFSLNARAQFSEDFYDNGTAMKCWVVPNKNPAVADIMRKVDCEKSSQIIDIFASELSRSSGCEPVRQTQRFIYADGPALNGHCGRLLCANLAGHWRVIGMCAGEGKNRAGVTKALYADIPHEFLRADNLNAVQRGAELDAAILDRFSIPISECRKELTPMTTRLGYVVGQYPRALRKTSIVPSIIHDNLWRKPETEPTILGKIDDRSPFPYDPYATIGEKFVQEVGPIDLSVGSDASLVVANIGSSWKAVGKPQCPTVLTWEVAINGDAAIPYCERLPLSTSEGYPDSIQRNFGEKGKKRFFDLKGENVRVPTPALMEELEVLERELQKEEVCLTCINTACAKDEKTAPKKVRVQPKTRIFEILPFQINIIIRRYLMFWMQLLMVAHDELPSKVGINVYSESWDRLLGRHTRLANHFTGDYSGFDTSTPRVLVYAIIDKINELADDGEVNQRTRRNIIRFVLNRYLISDGVLYEIHGGTPSGFAPTVMINSVVNEFYLKWSWIGLLKEAGYANQATLYAFHEATEISLYGDDNFVSVATPVASVYNLTTISNFLGRIGVKLGDGAKTGTIKPFIPLEEVDFLKRQFVADSGSTAILCPLKKISIEERLFYVRGGQDEIAALELNIATALCEAFFHGKEYFSFLEGKIIEAMRKSGVALSRPLPTMESVRAWYMSQRGNTKIRSPSFEGLGTMSGILNIGLAEARSVGGVACFSGIEFRGRSDDHLMVIPTYIPGGWRTKQQQTYISFVRDSEKMAQVIKRVAHFSTVVATDKSMAYLVAICIAYSRGSISRMEVRCHVQNLKVAEMLLCNQICNFL |
| Enzyme Length | 2081 |
| Uniprot Accession Number | Q9WAL8 |
| Absorption | |
| Active Site | ACT_SITE 1210; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 1255; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 1348; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 3.6.4.-; 3.4.22.-; 2.7.7.48 |
| Enzyme Function | FUNCTION: Picornain 3C-like protease is a thiol protease that probably cleaves the polyprotein. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 741..748; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00551 |
| Features | Active site (3); Chain (4); Domain (3); Nucleotide binding (1); Region (1); Transmembrane (2) |
| Keywords | ATP-binding;DNA replication;Host membrane;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 230,447 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |