IED ID | IndEnz0002000879 |
Enzyme Type ID | protease000879 |
Protein Name |
RNA1 polyprotein Cleaved into: Protease-cofactor; Putative helicase EC 3.6.4.- ; Picornain 3C-like protease 3C-like protease EC 3.4.22.- ; RNA-directed RNA polymerase EC 2.7.7.48 |
Gene Name | |
Organism | Tomato torrado virus (isolate Solanum lycopersicum/Spain/PRIToTV0301/-) (ToTV) |
Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Secoviridae Torradovirus Tomato torrado virus Tomato torrado virus (isolate Solanum lycopersicum/Spain/PRIToTV0301/-) (ToTV) |
Enzyme Sequence | MSFSKMFPGFNSVTEKCATSSSGSFFSELTASISNFSRTLSNVTKVSSQISSHIEDLKPSVTDAASSFTSTCNSVTKLLDKIMTLIEPFIKAYSFVASMYKSICDMVAKIVASIKDKFTLGFNWVLDKSEDVDVLVIAFLIFAISMLIIVFICPSSVLDGVVQMTHIVFNTVGNFFSALYKLDWLPTWSQKFSMMAQANVLPGESMSHSPLSQVVASLIAFGISTLVFVAVPGRPNGLSNPLSKILYSAGSGAQQCNQLFTLFRNMKDCTSQAFSWVLEIIVDIFGFKNPVLSAISATLSTDLFTWMEEVDAVCDPAHRLENFANPAFTIKLQHLREQALKISAYIATHPVAAFMSHRVTAAIXHLDKIYGENCQHTGVGQYRAEPFMVQWYGASGCGKSTSMRLFINDVLDRMEEPKLNRLYAVSKRDAYWSNYAHQTAILMDDMGALRDGAGQCQDIKDLIDIKSTQPAPLPMAAVEDKGRHFTSRYIFATSNLISAPAQCGLTYPDAFERRRDVLVECRKVGEFNTDAPTSHLEFDVVESKRPHAITHRGLSYDDLLEYVVAKCKVHAEISGKLYGATSGKVAQVDVSPEEIIASMDMLNIQDTKQDAKLPVVVVSEEDRVAYSQELTVEALKYAYQGSLNPAAYFPHDMHKQAIFDVLSESAKETFTRWVNDMLYQGCCNENYRWLIKNIPADYIMHFKSFIYASTINERSFDVQKQLPDGMAHRAIDADVDTLICVEQMPAHVQFLYTAFVRYWCRRKMEQPRQSWVVVCYHSIVDYIKNAWYDLPYILRVLIKAGLILIALNGAFGAVTAFCACWQSNTFPSAEGRGGITNESNSISSRKNKGKSIFARSLLAQAKGDMLEKWASDDGFINEGLKKNLVVLRLGEGVYFRGTYVCSGWVMTVAHAFSSLRDGTTFSIIHAQSISKVQYNAKTARFLKEQDIVLLNVGNPDGPKPDIRKHFPVRDGVCFSKGTQGVCVRAVASKDASQGNLEYLRFNVMMSKGYLEKVTYQMDSSSFKLESQASYEYHMNGENGDCGTLLLLPNVQDKQPCIVGIHCASYDEEAAHKGFVASNATAIFRDQLEDLPTGPVKVAMVRCQLLKDLRARDAALFEEKQVAFVGTLPAEQAATVPHKTTLRRSGLFEAFGPAETAPSIISASDKRGEGFDPYVAGIQKYNETAQNFDEDIARLAYEGLRQAILPVLHSQRVPFGKPVTQNEDVVLNGVDGFDYFDGMELSTSCGYPYNKLGMGTSKREFVEPSGDGDRVQLKRTTPIFDDWEALDVEIRKGNFVELVTTQCAKDERLPLEKVFGKRKTRLFEILPFHYNMLVRKYFLDFSASLMASHNALPCKVGINPGGIEWTLLANGFRAVSDTGFSADYSSFDGRAPIFAFQWFCDLVDDYYGSPPGSPDSNARHVLLMMASCHYTICENKVFRLVGGMPSGFALTVIFNSLLNEFYMRYAFISLLRRPHIAAQAIGCKPSDFNKLFVAVYGDDNLVAVPMELHWYTLPAIAQELEMVNVIIKNGIDKNMDVSSSKMLDLSELTFLSRGFKRHRLGYVQAPLKWVSIIEPMYWIRPSVGCPDALAMLENIDTGVREAFHHGPQVFEKLVTDVQNALKERCFPATTFPTYFELEQDWLVEVTGNPAIGLIKELHIAASAFVPLPPGNTVLNFSDGVHTFADRVSFCSSRTAAAQQWDTTTVLVNCTGAKRPTWVRGPTTWRDFEGLIWPYTMAAIKDHICSIVTKGVTKPHVVFVCGNGYAIGPVCAALYCLSTGQYSSQDVVVRLRTIADVTDLSQYPGGCAKYLLKCADTREEELADTCKIAQAKGETPAYIPQGGFSLGNFRIVQGRIDLQLAQRLPFTVGPYGGWGQHTTRELKLLLKDMEKIYQILVQRESFITLYFDYLSSEQVMLLVDFLRLQGFFPRQNDVDYLLKAFKLSKQRHNKENCHTVYFRKPFLSRKMTMGSKEILSATAAESLFGMDVSANVLKSRLLHLQKPIKCSSMELAFKIYCVIQGHLSKEVVTHFQRMYQQDLTEGIIEKVILWLTATLSESFPVDLVDVPLGLDNIEIQDKGFSLNPNNINMNACDAILFQLTECYNRSTKKHVFCRYTTASSLVVAYVLAHRHQTIDELPSFYATHPDVLLLTPILTGYKAP |
Enzyme Length | 2158 |
Uniprot Accession Number | A1XIP9 |
Absorption | |
Active Site | ACT_SITE 910; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 946; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222; ACT_SITE 1041; /note=For picornain 3C-like protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01222 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
DNA Binding | |
EC Number | 3.6.4.-; 3.4.22.-; 2.7.7.48 |
Enzyme Function | FUNCTION: Picornain 3C-like protease is a thiol protease that probably cleaves the polyprotein. {ECO:0000250}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | NP_BIND 393..400; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00551 |
Features | Active site (3); Chain (4); Domain (3); Modified residue (1); Nucleotide binding (1); Propeptide (1); Site (3); Transmembrane (1) |
Keywords | ATP-binding;Covalent protein-RNA linkage;DNA replication;Helicase;Host membrane;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;Phosphoprotein;Protease;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: [Putative helicase]: Host membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. |
Modified Residue | MOD_RES 844; /note=O-(5'-phospho-RNA)-serine; /evidence=ECO:0000250 |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 241,087 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:21248 |
Cross Reference Brenda |