| IED ID | IndEnz0002000887 |
| Enzyme Type ID | protease000887 |
| Protein Name |
Genome polyprotein 2 Cleaved into: Helper component proteinase HC-pro EC 3.4.22.45 ; 70 kDa protein |
| Gene Name | RNA2 |
| Organism | Barley mild mosaic virus (strain UK-F) (BaMMV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Stelpaviricetes Patatavirales Potyviridae Bymovirus Barley mild mosaic virus Barley mild mosaic virus (strain UK-F) (BaMMV) |
| Enzyme Sequence | MMMNSTIRQGWQQVLRRFSIPASGDRLIVSNSTDQPIGLFGAFDTSLQTLSQVTNDPEILKQKSNIPTHLDIASVLETSPRSFPWVFLTNSFCTFGGSIHAQNLQAFATAEFKSGFCYMNLLIPLSFDIIDAHADSFRGFVEQLPDTLGAYPSLSMVLNVMLHAATRFPEIVASPVPTIAFDAESLQFHVTDKRGVPGMWNILKACRVYELLSLAADGIGCEYMLYPVGAAPQYSFWKKSMDHFISDRFVEFLAMQGLLASALEQDYKTHDARDALLTALQNAGYTNVVARERRFPNGHDPSTVWLNLNEAPISEKLTELKRYLLVGHRSDEIADITHNVHQHVFEVLKTMSVQFSKTTNAYNRARFEVNHEVIWNAEYGRSSQQNAELEALVLFLNRQSLEIENILRRTTSPVVVTNWQPDVPPAAPEISEGEPTHAVATPITEAPTHATPVEVVNLPPTRSYWAETLVGVLTAILGTIFALLTRALIRPKRLRRKSTFPWVSLDSGDEDDDHSGGGGGSPQTPGGQPPASPAPGTHQSRFSVQDIASDTSLLSVDLDEDTLSQYDETFQTIRRALFENSFGDILQNSARWISTLEAMALADGNAPYTLLAQYLNGIEEAYTNFRNTGHISRATLSGFFVLEDSLRAAGIAFGGTTPTQTIQNQSADSPARRWKTRFEQIACELGDASIKSLADLADIIDTERERGDLTQFDVLAASSISSLCRAVRIISDTTDPNTQLALVENATAMQNNINAILGTNVSIPFLSATRRLLITRRIQEAGAESRSGATPDTIQQLADAELAEIVSEANMFNEMAASQRDIANATREATIREHVLSPVNALANVGMAAAFFRSGGLRSRAFHPTMPTMPGSPAAIGRPMFQAFRGRGHRLNRR |
| Enzyme Length | 894 |
| Uniprot Accession Number | Q65657 |
| Absorption | |
| Active Site | ACT_SITE 117; /note=For helper component proteinase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01080; ACT_SITE 189; /note=For helper component proteinase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01080 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.; EC=3.4.22.45; |
| DNA Binding | |
| EC Number | 3.4.22.45 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (2); Domain (1); Region (1); Site (1) |
| Keywords | Hydrolase;Protease;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: The viral RNA2 of bymoviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least two individual proteins. The HC-pro cleaves its C-terminus autocatalytically (Potential). {ECO:0000305}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 98,349 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |