IED ID | IndEnz0002000887 |
Enzyme Type ID | protease000887 |
Protein Name |
Genome polyprotein 2 Cleaved into: Helper component proteinase HC-pro EC 3.4.22.45 ; 70 kDa protein |
Gene Name | RNA2 |
Organism | Barley mild mosaic virus (strain UK-F) (BaMMV) |
Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Stelpaviricetes Patatavirales Potyviridae Bymovirus Barley mild mosaic virus Barley mild mosaic virus (strain UK-F) (BaMMV) |
Enzyme Sequence | MMMNSTIRQGWQQVLRRFSIPASGDRLIVSNSTDQPIGLFGAFDTSLQTLSQVTNDPEILKQKSNIPTHLDIASVLETSPRSFPWVFLTNSFCTFGGSIHAQNLQAFATAEFKSGFCYMNLLIPLSFDIIDAHADSFRGFVEQLPDTLGAYPSLSMVLNVMLHAATRFPEIVASPVPTIAFDAESLQFHVTDKRGVPGMWNILKACRVYELLSLAADGIGCEYMLYPVGAAPQYSFWKKSMDHFISDRFVEFLAMQGLLASALEQDYKTHDARDALLTALQNAGYTNVVARERRFPNGHDPSTVWLNLNEAPISEKLTELKRYLLVGHRSDEIADITHNVHQHVFEVLKTMSVQFSKTTNAYNRARFEVNHEVIWNAEYGRSSQQNAELEALVLFLNRQSLEIENILRRTTSPVVVTNWQPDVPPAAPEISEGEPTHAVATPITEAPTHATPVEVVNLPPTRSYWAETLVGVLTAILGTIFALLTRALIRPKRLRRKSTFPWVSLDSGDEDDDHSGGGGGSPQTPGGQPPASPAPGTHQSRFSVQDIASDTSLLSVDLDEDTLSQYDETFQTIRRALFENSFGDILQNSARWISTLEAMALADGNAPYTLLAQYLNGIEEAYTNFRNTGHISRATLSGFFVLEDSLRAAGIAFGGTTPTQTIQNQSADSPARRWKTRFEQIACELGDASIKSLADLADIIDTERERGDLTQFDVLAASSISSLCRAVRIISDTTDPNTQLALVENATAMQNNINAILGTNVSIPFLSATRRLLITRRIQEAGAESRSGATPDTIQQLADAELAEIVSEANMFNEMAASQRDIANATREATIREHVLSPVNALANVGMAAAFFRSGGLRSRAFHPTMPTMPGSPAAIGRPMFQAFRGRGHRLNRR |
Enzyme Length | 894 |
Uniprot Accession Number | Q65657 |
Absorption | |
Active Site | ACT_SITE 117; /note=For helper component proteinase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01080; ACT_SITE 189; /note=For helper component proteinase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01080 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.; EC=3.4.22.45; |
DNA Binding | |
EC Number | 3.4.22.45 |
Enzyme Function | |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Chain (2); Domain (1); Region (1); Site (1) |
Keywords | Hydrolase;Protease;Thiol protease |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | PTM: The viral RNA2 of bymoviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least two individual proteins. The HC-pro cleaves its C-terminus autocatalytically (Potential). {ECO:0000305}. |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 98,349 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |