Detail Information for IndEnz0002000889
IED ID IndEnz0002000889
Enzyme Type ID protease000889
Protein Name Genome polyprotein 2
Cleaved into: Helper component proteinase
HC-pro
EC 3.4.22.45
; 70 kDa protein
Gene Name RNA2
Organism Barley yellow mosaic virus (strain Japanese II-1) (BaYMV)
Taxonomic Lineage Viruses Riboviria Orthornavirae Pisuviricota Stelpaviricetes Patatavirales Potyviridae Bymovirus Barley yellow mosaic virus (BaYMV) Barley yellow mosaic virus (strain Japanese II-1) (BaYMV)
Enzyme Sequence MSASSSRQLFDCGSLDWPNKSLFGDPTTRDVMHEHISSTWNAVIRRHMLAPNADAETILGRDGLPSAQFDAYGAMLPSFIQALNAPTTRLRITHRCPTAESILCADASHAPWLYMANNVCAYEATHLKPVQTFIAFDFAHGYCYLSLFIPLSFRITFENARSFSRFLEQLPDILGAYPTLAAIYKTMLFAIRLFPEVLQAPIPIIAKRPGVLQFHVSDARGLPPSWFPMKCGSVRSFVALITNNLNSDLLDGIVGSNGDGEHYTNWNSGHDHWIVNRFITVRDLHSSLKSALDVDLDTEGGRNAVLDLLLDLGVTNLVRREKRFPAHFQGAESVYLLLSCERVGNELVAVQDALQEPLANHSGLDLRALIINLGGLPSRHSDICYTRNIFENDNHLVWNFEFYRIASITRNAQIDRDMLSSSMANLFSNFVSESSNGQYRVKEPRPIAQYRVEHDEPVASGAPSAWWQVLIGITTAILGAIIFFLWRCFLRAKRVKFQAKDSFPWFTTSGDDDSPPPPGDSPSHPPGRSPDRVLPRTVVRDLSFNDDDDLHSVDLDEAGSRFGEVVSLIARGNLRELAGAIPESLSNLTLLQTSASGSGFYTMVALYLATLGDAITAFHEHNDASPTTTQSLRTVELQLEARGLRFNEAGTPANLIQRGVNSSVGRALVRLTQSALLATGEKFRTRMATTLERIAAERLNTLTAYDQRVIEMTTELLAAIKTALEVERSELTPHLANAEALLQVYNNLFSTDYASASLLALRREMILRSAEGRVGEQPTSASDAANEELVQRSMTKLDKEIELFQAQIDSQRRAVTITEASNLRENILQPINTVANIAMAGAFLRGGARHRMPGMPDVAAPMPNPFRAFSGRGHSLTTTRSGGLFRRPRV
Enzyme Length 890
Uniprot Accession Number Q01207
Absorption
Active Site ACT_SITE 143; /note=For helper component proteinase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01080; ACT_SITE 215; /note=For helper component proteinase activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01080
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.; EC=3.4.22.45;
DNA Binding
EC Number 3.4.22.45
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (2); Compositional bias (1); Domain (1); Region (1); Site (1)
Keywords Hydrolase;Protease;Thiol protease
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification PTM: The viral RNA2 of bymoviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least two individual proteins. The HC-pro cleaves its C-terminus autocatalytically (Potential). {ECO:0000305}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 98,464
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda