| IED ID | IndEnz0002000891 |
| Enzyme Type ID | protease000891 |
| Protein Name |
RNA2 polyprotein 119kDa protein Genome polyprotein M Cleaved into: VP53 53 kDa protein ; Movement protein MP 37 kDa protein VP37 ; Large capsid protein LCP 44 kDa protein ; Small capsid protein SCP 22 kDa protein |
| Gene Name | |
| Organism | Broad bean wilt virus 2 (BBWV-2) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Secoviridae Comovirinae Fabavirus Broad bean wilt virus 2 (BBWV-2) |
| Enzyme Sequence | MRPELVAVLDRYFSEIISCFFLGWLLNFLLVWFCSTKSTFLLWSVFLYICYYILRIEFAYIVAPFLKTIYTNSSQYHTVDWAYAYTALPKGLWEQITDYNYCYNFPPPRVEGFVSDFSPRFTLKELEIMNEANITPVHTIPKDTLLKRASDYKLAVESKKSILPRVQDLYEMDKWHNLRSKLSKNAPSYVTTSEIAVGAMSGAGNTKLAIPVVEKYTEEVADDRLPDRVRAKADQIMVAAIELVADGFASVNSDVTMAGALYDKRHKTIASSFKGAFASRASGVPSHVIYFPMHRVPACDDPNTTLELSMVSRDSDFDEGYTLANISARTLYVRAKGPEKVTETRHLLKAKTEDVVKARQFASEAQVVFATPRLFPEVNLDNYNLPGPSNAQQTEAITTDRGILFPKPKFKGNEVVLNYTGSGKIRNVGSQRFETKKNATGEQFVRSVDDLGCLSDEDGKDYRYGQGLMEEDVLNVQTNNFAIESATETMRLLFSGYASIPLNVIPGTKITVAYLNELSKHSAVHTGLLNMLSKIPGSLKVKINCQVAPTCGIGLAVSYVEGNESANLGSSLGRLLGIQHYKWNPAIEPYVEFVFKPFSCADWWNMHYLGSFKFAPVVVIQTLSKWLNAPKVDARISFAIYYEPTVVLPKQIATLEHAPAFMFRKEVGTLAFKQGERVAYSFEVNLGKPQTDGKEVTSTFASSYCGLSQYMQSDVILDFTLMSSPMIGGTFSIAYVAGAYIEKVGNMQILDSLPHIDFTFSSGSKSTRSVRFPKEVFGVYQALDRWDLDSARGDDVSGNFVLYQRDAVSSALEGELTFRIAARLSGDISFTGVSAGYPTTITRIGKGKTIGRSLDPEIRKPLRYMLGQAHATPKDFSSVRFVMGHWKYRAGLYPGSKSDEDIHPFSLKMRLDGSKSSENFEIIHSPFVRLLQNCAWMRGTLRFYVVARASSDYMSYRRTSQLTVSAHENSLSSNQFYSGVLTSPSGELSFSREVVGPVDGFASMGWNVRGSKKFYKIHVEMGNVHEYDTVVLYGQFDSNVEFAGQRKGGHYLLEKETPIFKTIKY |
| Enzyme Length | 1065 |
| Uniprot Accession Number | Q9Q2Q3 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: [VP53]: Acts as suppressor of post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs (PubMed:25173697). Binds ssRNA (PubMed:25173697). {ECO:0000269|PubMed:25173697}.; FUNCTION: [Movement protein]: Transports the viral genome to neighboring plant cells directly through plasmosdesmata, without any budding (PubMed:19463725). The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier (PubMed:19463725). Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids (PubMed:20832435, PubMed:26903400). Binds to GTP and to single-stranded RNA and single-stranded DNA in a non-sequence-specific manner (PubMed:12021864). Also acts as suppressor of post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs (PubMed:25173697). {ECO:0000269|PubMed:12021864, ECO:0000269|PubMed:19463725, ECO:0000269|PubMed:20832435, ECO:0000269|PubMed:25173697, ECO:0000269|PubMed:26903400}.; FUNCTION: [Large capsid protein]: Together with the small capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms (By similarity). The large capsid protein interacts with the viral RNA (By similarity). Also acts as suppressor of post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs (PubMed:25173697). Binds ssRNA (PubMed:25173697). {ECO:0000250|UniProtKB:P03599, ECO:0000269|PubMed:25173697}.; FUNCTION: [Small capsid protein]: Together with the large capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms (By similarity). The capsid is formed from 60 copies each of the large and the small capsid protein. The small capsid protein forms the turrets at the fivefold axes of the viral particle (By similarity). {ECO:0000250|UniProtKB:P03599}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (1); Chain (5); Site (2) |
| Keywords | Alternative initiation;Capsid protein;DNA-binding;Host cell junction;Host endoplasmic reticulum;RNA-binding;Suppressor of RNA silencing;T=3 icosahedral capsid protein;Transport;Viral movement protein;Virion |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Movement protein]: Host endoplasmic reticulum {ECO:0000269|PubMed:19463725}. Host cell junction, host plasmodesma {ECO:0000269|PubMed:19463725, ECO:0000269|PubMed:20832435, ECO:0000269|PubMed:26903400}. Note=Assembles in tubules that are embedded within modified plasmodesmata. {ECO:0000250|UniProtKB:P03599}.; SUBCELLULAR LOCATION: [Large capsid protein]: Virion {ECO:0000250|UniProtKB:P03599}.; SUBCELLULAR LOCATION: [Small capsid protein]: Virion {ECO:0000250|UniProtKB:P03599}. |
| Modified Residue | |
| Post Translational Modification | PTM: [RNA2 polyprotein]: Specific enzymatic cleavages by picornain 3C-like protease in vivo yield mature proteins. {ECO:0000250|UniProtKB:P03599}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 119,214 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |