Detail Information for IndEnz0002000893
IED ID IndEnz0002000893
Enzyme Type ID protease000893
Protein Name RNA2 polyprotein
Genome polyprotein M
M RNA polyprotein
Middle component RNA polyprotein
P2

Cleaved into: VP58
P58
; Movement protein
MP
; Large capsid protein
LCP
Coat protein VP42
L subunit
Large coat protein
; Small capsid protein precursor
S subunit
; Mature small capsid protein
SCP
Coat protein VP22
Small capsid protein, N-terminus part
Small coat protein, N-terminus part
; Small capsid protein C-terminus part
Small coat protein C-terminus part
Gene Name
Organism Bean-pod mottle virus (strain Kentucky G7) (BPMV)
Taxonomic Lineage Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Secoviridae Comovirinae Comovirus Bean pod mottle virus Bean-pod mottle virus (strain Kentucky G7) (BPMV)
Enzyme Sequence MFASFIFSGDNKLTEKTIFNCGDLDILVVYYTIATQFRKFLPHYIRWHLYTLLIYILPSFLTTEIKYKRNLSNIHISGLFYDNRFKFWTKHDKNLALTEEEKMEVIRNRGIPADVLAKRAHEFEKHVAHESLKDQIPAVDKLYSTKVNKFAKIMNLRQSVVGDLKLLTDGKLYEGKHIPVSNISAGENHVVQIPLMAQEEILSSSASDFKTAMVSKSSKPQATAMHVGAIEIIIDSFASPDCNIVGAMLLVDTYHTNPENAVRSIFVAPFRGGRPIRVVTFPNTIVQIEPDMNSRFQLLSTTTNGDFVQGKDLAMVKVNVACAAVGLTSSYTPTPLLESGLQKDRGLIVEYFGRMSYVAHNINQPQEKDLLEGNFSFDIKSRSRLEKVSSTKAQFVSGRTFKYDIIGAGSQSSEELSEEKIQGKAKQVDARLRQRIDPQYNEVQAQMETNLFKLSLDDVETPKGSMLDLKISQSKIALPKNTVGGTILRSDLLANFLTEGNFRASVDLQRTHRIKGMIKMVATVGIPENTGIALACAMNSSIRGRASSDIYTICSQDCELWNPACTKAMTMSFNPNPCSDAWSLEFLKRTGFHCDIICVTGWTATPMQDVQVTIDWFISSQECVPRTYCVLNPQNPFVLNRWMGKLTFPQGTSRSVKRMPLSIGGGAGAKSAILMNMPNAVLSMWRYFVGDLVFEVSKMTSPYIKCTVSFFIAFGNLADDTINFEAFPHKLVQFGEIQEKVVLKFSQEEFLTAWSTQVRPATTLLADGCPYLYAMVHDSSVSTIPGDFVIGVKLTIIENMCAYGLNPGISGSRLLGTIPQSISQQTVWNQMATVRTPLNFDSSKQSFCQFSVDLLGGGISVDKTGDWITLVQNSPISNLLRVAAWKKGCLMVKVVMSGNAAVKRSDWASLVQVFLTNSNSTEHFDACRWTKSEPHSWELIFPIEVCGPNNGFEMWSSEWANQTSWHLSFLVDNPKQSTTFDVLLGISQNFEIAGNTLMPAFSVPQANARSSENAESSA
Enzyme Length 1018
Uniprot Accession Number P23009
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: [VP58]: Responsible for viral RNA2 accumulation. May function by recruiting the RNA1-encoded polyprotein that contains the replication protein to RNA2 and enable its replication. {ECO:0000269|PubMed:24390330}.; FUNCTION: [Movement protein]: Transports the viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids. Binds to GTP and to single-stranded RNA and single-stranded DNA in a non-sequence-specific manner. {ECO:0000250|UniProtKB:P03599}.; FUNCTION: [Small capsid protein precursor]: The cleavable C-terminus of small capsid protein seems to be involved in viral assembly and RNA packaging. After virus assembly, these amino acids are cleaved off during the normal maturation of the virus. Also seems to act as suppressor of post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. {ECO:0000250|UniProtKB:P03599}.; FUNCTION: [Small capsid protein C-terminus part]: Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. {ECO:0000250|UniProtKB:P03599}.; FUNCTION: [Large capsid protein]: Together with the mature small capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms. The capsid is formed from 60 copies each of the large and the mature small capsid protein. The large capsid protein interacts with the viral RNA. {ECO:0000269|PubMed:14517057, ECO:0000269|PubMed:2749253}.; FUNCTION: [Mature small capsid protein]: Together with the large capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms. The capsid is formed from 60 copies each of the large and the mature small capsid protein. The mature small capsid protein forms the turrets at the fivefold axes of the viral particle. {ECO:0000269|PubMed:14517057, ECO:0000269|PubMed:2749253}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Alternative sequence (1); Beta strand (37); Chain (7); Helix (10); Region (1); Site (4); Turn (8)
Keywords 3D-structure;Alternative initiation;Capsid protein;DNA-binding;GTP-binding;Host cell junction;Nucleotide-binding;RNA-binding;Suppressor of RNA silencing;T=3 icosahedral capsid protein;Transport;Viral movement protein;Virion
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: [Movement protein]: Host cell junction, host plasmodesma {ECO:0000250|UniProtKB:P03599}. Note=Assembles in tubules that are embedded within modified plasmodesmata. {ECO:0000250|UniProtKB:P03599}.; SUBCELLULAR LOCATION: [Large capsid protein]: Virion {ECO:0000269|PubMed:14517057, ECO:0000269|PubMed:2749253}.; SUBCELLULAR LOCATION: [Mature small capsid protein]: Virion {ECO:0000269|PubMed:14517057, ECO:0000269|PubMed:2749253}.
Modified Residue
Post Translational Modification PTM: [Small capsid protein precursor]: The C-terminal amino acids of the small capsid protein is specifically cleaved by the RNA1 encoded picornain 3C-like protease during maturation. {ECO:0000250|UniProtKB:P03599, ECO:0000305|PubMed:1716655}.; PTM: [RNA2 polyprotein]: Specific enzymatic cleavages by picornain 3C-like protease in vivo yield mature proteins. {ECO:0000250|UniProtKB:P03599}.
Signal Peptide
Structure 3D X-ray crystallography (3)
Cross Reference PDB 1BMV; 1PGL; 1PGW;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 113,352
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda