| IED ID | IndEnz0002000894 |
| Enzyme Type ID | protease000894 |
| Protein Name |
RNA2 polyprotein P2 Cleaved into: Protein 2A; Movement protein MP ; Coat protein CP |
| Gene Name | |
| Organism | Blackcurrant reversion association virus (BRAV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Secoviridae Comovirinae Nepovirus Blackcurrant reversion association virus (BRAV) |
| Enzyme Sequence | MSESGNTTSMPGCGRMCALRSTWSKRAFLVACKDGALTSDGRCPQYGCGALVSITKGVQQPKKTASAKVVKCLCWVQPARWCEKHSKGPASPNGSVTTKRSNSARAAPAPLPYKKQTCDVVVTVGPLELVYPALVSEELPTPVAATPTKVEEVPIPELPLWLAPAWMVEQPYAATPEVLCLTQREEFALLKKRLTRKGKLLQRRATHARFEARAALARVRAATQRKVEEVTALVIKGRRILAAHQLLRELEEVAPLSQAQEQLVASSCAAAAARQEECASFLRRAKAWRKSISATPPVAFATAVASKVVSATMPWAHLGLSLGGLLAVPTLDGTLGAKQWNAKTIATWVLKPVVSCVQSVHAKVRDWLHSQPEVGVTNTKVPLVLPEVCLGVLSPPSLSEEIVDNPQETSQSGIWHPEMGVRNIYVFHDDSWETSPEEDENYTYTFSRQCGIPYLLVEGRGAEERKNTILGWDFSLHNDGEFEFLPSPEEGYTKELVTPVALEEEDKYSTASSCGFFSLDDVSSAITIQCPGLLSADADVHFFDGPGYRCSSRPRDFRPPVVRGCDYESRVKASIQRKIENPLQERFITVLREKRKKNKKKEFHSFSACFAFKRKQIQWPPTPNEMVNEWEEYCIAQAWLPFEVVVTDEIEDVTPLYPGGRDYNCNSQLLFPLAPLSTVYCDDSCFHPNDGWTTDGNGKHFRLSPQFVLPDVPIPIVHRVTRQLPQFLYDLGIGDLTCNSGYQAENLQEEIQERMEDRSEEKPVPSLDTLISKLSKRSTKVKGAGENRYADRHSLTEKAIFHQPGALSRMRSGKEKTIVAANHNSDQISVRMAECGKPVFTPLPRMSDEMLRKFLEKGLGSTSTVALDIGIQSHIPQGMPTVAFVNVMDTRIEDPLYSSLCGSYIDLGRDRAKTLCLPLVNFPMSKLAEDVDDVLNGLMLCTHFQDSTKFGVGKPAFQYGTLEFQEFKPSAYSDFSRVRDNWDAIAKQQNTPNDRILAGFSVLGAVSQAYNQALPVFKSVELVAPPKRKPVVATFQNPTTLGRSNTTRSFRMPTMDLPRSTGRDAPIPIVHRRNNNDVHGFDEATPARFSTCDSGLVADTTLAFAKMYQCKKDAKAGHVLATIDIQECVFEDNRRVALDWLAHGLASFKYDLQLTVDSNPFVGVTLGITVDAFDRLLPQISDEVIAVPLAFQLPTYLFPISKKGTFTQTIDFAAIAGYNFFPHVAAFGRPKIIVYIVSDNDLPASDTWMCLVELHMTRLESSTLACSPTLVLPQAFGGDLPLDLWRGPYTFPLGGGTKRLSTSLDIGTSTTTVSGWRTVSFPAAYALFLQGHGGSLVGEVVHTGSAAVSCALHLCISFGGAPPTLEEALVFPGFRLPSGEGKFHIKVQTPYGRLSTLTPDCALYVYLAGGPIAVAPMSVPYQFCIHLERLVDDGAPPRTIGLIREFNWATINNFKSDDITFAIPARLSDLVLTCGDVTMSTNPLALLIGSCGFFRGNLTVVLEWATFLKAGDKEGTVQLTTCRGMINNVKGVRNAIQKKVVNLSLVGSVSRYLNVGDFTGFAQSGGQVGYDEIFLEFSTNKAKQIRYLNINVELDENFELYGRTIIPLKNTAPAFASTSASAPNES |
| Enzyme Length | 1626 |
| Uniprot Accession Number | Q9YK98 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: [Protein 2A]: Implicated in RNA2 replication. Could also be required for nematode transmission of the virus (By similarity). {ECO:0000250}.; FUNCTION: [Movement protein]: Transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (3); Compositional bias (1); Region (2) |
| Keywords | Capsid protein;Direct protein sequencing;Host cell junction;Transport;Viral movement protein;Virion |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Movement protein]: Host cell junction, host plasmodesma {ECO:0000250}. Note=Assembles in tubules that are embedded within modified plasmodesmata. {ECO:0000250}.; SUBCELLULAR LOCATION: [Coat protein]: Virion {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages in vivo by the P1 encoded 3C-like protease yield mature proteins. {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18556038; |
| Motif | |
| Gene Encoded By | |
| Mass | 178,806 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |