| IED ID | IndEnz0002000897 |
| Enzyme Type ID | protease000897 |
| Protein Name |
RNA2 polyprotein Genome polyprotein M M RNA polyprotein Middle component RNA polyprotein P2 Cleaved into: VP58 P58 ; Movement protein MP 48 kDa protein ; Large capsid protein LCP Coat protein VP37 L subunit Large coat protein ; Small capsid protein precursor S subunit ; Mature small capsid protein SCP Coat protein VP23 Small capsid protein, N-terminus part Small coat protein, N-terminus part ; Small capsid protein C-terminus part Small coat protein C-terminus part |
| Gene Name | |
| Organism | Cowpea mosaic virus (strain SB) (CPMV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Secoviridae Comovirinae Comovirus Cowpea mosaic virus (CPMV) Cowpea mosaic virus (strain SB) (CPMV) |
| Enzyme Sequence | MFSFTEAKSKISLWTRSAAPLNNVYLSYSCRCGLGKRKLAGGCCSAPYITCYDSADFRRVQYLYFCLTRYCCLYFFLLLLADWFYKKSSIFFETEFSRGFRTWRKIVKLLYILPKFEMESIMSRGIPSGILEEKAIQFKRAKEGNKPLKDEIPKPEDMYVSHTSKWNVLRKMSQKTVDLSKAAAGMGFINKHMLTGNILAQPTTVLDIPVTKDKTLAMASDFIRKENLKTSAIHIGAIEIIIQSFASPESDLMGGFLLVDSLHTDTANAIRSIFVAPMRGGRPVRVVTFPNTLAPVSCDLNNRFKLICSLPNCDIVQGSQVAEVSVNVAGCATSIEKSHTPSQLYTEEFEKEGAVVVEYLGRQTYCAQPSNLPTEEKLRSLKFDFHVEQPSVLKLSNSCNAHFVKGESLKYSISGKEAENHAVHATVVSREGASAAPKQYDPILGRVLDPRNGNVAFPQMEQNLFALSLDDTSSVRGSLLDTKFAQTRVLLSKAMAGGDVLLDEYLYDVVNGQDFRATVAFLRTHVITGKIKVTATTNISDNSGCCLMLAINSGVRGKYSTDVYTICSQDSMTWNPGCKKNFSFTFNPNPCGDSWSAEMISRSRVRMTVICVSGWTLSPTTDVIAKLDWSIVNEKCEPTIYHLADCQNWLPLNRWMGKLTFPQGVTSEVRRMPLSIGGGAGATQAFLANMPNSWISMWRYFRGELHFEVTKMSSPYIKATVTFLIAFGNLSDAFGFYESFPHRIVQFAEVEEKCTLVFSQQEFVTAWSTQVNPRTTLEADGCPYLYAIIHDSTTGTISGDFNLGVKLVGIKDFCGIGSNPGIDGSRLLGAIAQGPVCAEASDVYSPCMIASTPPAPFSDVTAVTFDLINGKITPVGDDNWNTHIYNPPIMNVLRTAAWKSGTIHVQLNVRGAGVKRADWDGQVFVYLRQSMNPESYDARTFVISQPGSAMLNFSFDIIGPNSGFEFAESPWANQTTWYLECVATNPRQIQQFEVNMRFDPNFRVAGNILMPPFPLSTETPPLLKFRFRDIERSKRSVMVGHTATAA |
| Enzyme Length | 1046 |
| Uniprot Accession Number | P03599 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: [VP58]: Responsible for viral RNA2 accumulation. May function by recruiting the RNA1-encoded polyprotein that contains the replication protein to RNA2 and enable its replication. {ECO:0000250|UniProtKB:P23009}.; FUNCTION: [Movement protein]: Transports the viral genome to neighboring plant cells directly through plasmosdesmata, without any budding (PubMed:9501035). The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids (PubMed:12556992, PubMed:14579172) (Probable). Binds to GTP and to single-stranded RNA and single-stranded DNA in a non-sequence-specific manner (PubMed:14722313). {ECO:0000269|PubMed:12556992, ECO:0000269|PubMed:14579172, ECO:0000269|PubMed:14722313, ECO:0000269|PubMed:9501035, ECO:0000305|PubMed:8497075}.; FUNCTION: [Large capsid protein]: Together with the mature small capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms (PubMed:28373698, PubMed:10603314, PubMed:26657148, PubMed:27021160). The capsid is formed from 60 copies each of the large and the small capsid protein (PubMed:28373698, PubMed:10603314, PubMed:26657148, PubMed:27021160). The large capsid protein interacts with the viral RNA (PubMed:28373698, PubMed:26657148). {ECO:0000269|PubMed:10603314, ECO:0000269|PubMed:26657148, ECO:0000269|PubMed:27021160, ECO:0000269|PubMed:28373698}.; FUNCTION: [Mature small capsid protein]: Together with the large capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms. The capsid is formed from 60 copies each of the large and the small capsid protein. The mature small capsid protein forms the turrets at the fivefold axes of the viral particle. {ECO:0000269|PubMed:10603314, ECO:0000269|PubMed:26657148, ECO:0000269|PubMed:27021160, ECO:0000269|PubMed:28373698}.; FUNCTION: [Small capsid protein precursor]: The cleavable C-terminus of small capsid protein seems to be involved in viral assembly and RNA packaging (PubMed:10049828). After virus assembly, these amino acids are cleaved off during the normal maturation of the virus (PubMed:26657148, PubMed:10049828). Also seems to act as suppressor of post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs (PubMed:15483261, PubMed:15165817). {ECO:0000269|PubMed:10049828, ECO:0000269|PubMed:15165817, ECO:0000269|PubMed:15483261, ECO:0000269|PubMed:26657148}.; FUNCTION: [Small capsid protein C-terminus part]: Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. {ECO:0000269|PubMed:15165817, ECO:0000269|PubMed:15483261}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (1); Beta strand (36); Chain (7); Helix (11); Modified residue (1); Mutagenesis (21); Region (2); Site (7); Turn (6) |
| Keywords | 3D-structure;Acetylation;Alternative initiation;Capsid protein;DNA-binding;GTP-binding;Host cell junction;Host nucleus;Nucleotide-binding;RNA-binding;Reference proteome;Suppressor of RNA silencing;T=3 icosahedral capsid protein;Transport;Viral movement protein;Virion |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [VP58]: Host nucleus {ECO:0000269|PubMed:8497075}.; SUBCELLULAR LOCATION: [Movement protein]: Host cell junction, host plasmodesma {ECO:0000269|PubMed:14579172, ECO:0000269|PubMed:27339685}. Note=Assembles in tubules that are embedded within modified plasmodesmata. {ECO:0000269|PubMed:10864669, ECO:0000269|PubMed:14579172, ECO:0000269|PubMed:27339685}.; SUBCELLULAR LOCATION: [Large capsid protein]: Virion {ECO:0000269|PubMed:10603314, ECO:0000269|PubMed:26657148, ECO:0000269|PubMed:27021160, ECO:0000269|PubMed:28373698}.; SUBCELLULAR LOCATION: [Mature small capsid protein]: Virion {ECO:0000269|PubMed:10603314, ECO:0000269|PubMed:26657148, ECO:0000269|PubMed:27021160, ECO:0000269|PubMed:28373698}. |
| Modified Residue | MOD_RES 460; /note=N-acetylmethionine; by host; /evidence=ECO:0000269|PubMed:6641721 |
| Post Translational Modification | PTM: [RNA2 polyprotein]: Specific enzymatic cleavages by picornain 3C-like protease in vivo yield mature proteins. {ECO:0000269|PubMed:16789216}.; PTM: [Small capsid protein precursor]: The C-terminal 24 amino acids of the small capsid protein are specifically cleaved by the RNA1 encoded picornain 3C-like protease during maturation. {ECO:0000269|PubMed:10049828, ECO:0000269|PubMed:27021160}.; PTM: [Large capsid protein]: Not glycosylated. {ECO:0000269|PubMed:10725439}.; PTM: [Mature small capsid protein]: Not glycosylated. {ECO:0000269|PubMed:10725439}. |
| Signal Peptide | |
| Structure 3D | Electron microscopy (2); X-ray crystallography (3) |
| Cross Reference PDB | 1NY7; 2BFU; 5A32; 5A33; 5FMO; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 116,218 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |