| IED ID | IndEnz0002000902 |
| Enzyme Type ID | protease000902 |
| Protein Name |
RNA2 polyprotein P2 Cleaved into: Protein 2A P2A ; Movement protein 2B-MP ; Coat protein 2C-CP |
| Gene Name | |
| Organism | Grapevine fanleaf virus (GFLV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Secoviridae Comovirinae Nepovirus Grapevine fanleaf virus (GFLV) |
| Enzyme Sequence | MGKFYYSNRRLACWAAGKNPHLGGSVEQWLAAINTDPSFRQTVKEDVQENREQPTAVRMFSWKVGSGPIDNPEKCDWHFVLTGERPAPSRPVKADEVVVVPQPKKVVIPTPPPPPAPYFRAVGAFAPTRSEFVRAIVERLTRLREESRAAALFAELPLEYPQGAPLKLSLAAKFAMLKHTTWRKWYDTSDERLLEAHPGGPCLPPPPPIQNPPSFQERVREFCRMKSCTKAFALETSLGLNKAWVGLVDIPSTSVCCADGKTTGGQTIAQEADPLQHRISTSVAPGRAQWISERRQALRRREQANSFEGLAAQTDMTFEQARNAYLGAADMIEQGLPLLPPLRSAYAPRGLWRGPSTRANYTLDFRLNGIPTGTNTLEILYNPVSEEEMEEYRDRGMSAVVIDALEIAINPFGMPGNPTDLTVVATYGHERDMTRAFIGSASTFLGNGLARAIFFPGLQYSQEEPRRESIIRLYVASTNATVDTDSVLAAISVGTLRQHVGSMHYRTVASTVHQAQVQGTTLRATMMGNTVVVSPEGSLVTGTPEARVEIGGGSSIRMVGPLQWESVEEPGQTFSIRSRSRSVRIDRNVDLPQLEAEPRLSSTVRGLAGRGVIYIPKDCQANRYLGTLNIRDMISDFKGVQYEKWITAGLVMPTFKIVIRLPANAFTGLTWVMSFDAYNRITSRITASADPVYTLSVPHWLIHHKLGTFSCEIDYGELCGHAMWFKSTTFESPRLHFTCLTGNNKELAADWQAVVELYAELEEATSFLGKPTLVFDPGVFNGKFQFLTCPPIFFDLTAVTALRSAGLTLGQVPMVGTTKVYNLNSTLVSCVLGMGGTVRGRVHICAPIFYSIVLWVVSEWNGTTMDWNELFKYPGVYVEEDGSFEVKIRSPYHRTPARLLAGQSQRDMSSLNFYAIAGPIAPSGETAQLPIVVQIDEIVRPDLSLPSFEDDYFVWVDFSEFTLDKEEIEIGSRFFDFTSNTCRVSMGENPFAAMIACHGLHSGVLDLKLQWSLNTEFGKSSGSVTITKLVGDKAMGLDGPSHVFAIQKLEGTTELLVGNFAGANPNTRFSLYSRWMAIKLDQAKSIKVLRVLCKPRPGFSFYGRTSFPV |
| Enzyme Length | 1109 |
| Uniprot Accession Number | P18474 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: [Protein 2A]: Implicated in RNA2 replication. Could also be required for nematode transmission of the virus.; FUNCTION: [Movement protein]: Transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (35); Chain (3); Helix (13); Turn (1) |
| Keywords | 3D-structure;Capsid protein;Direct protein sequencing;Host cell junction;Host cytoplasm;Host nucleus;Transport;Viral movement protein;Virion |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Host cell junction, host plasmodesma {ECO:0000269|PubMed:10544127, ECO:0000269|PubMed:12953111}. Note=Assembles in tubules that are embedded within modified plasmodesmata (By similarity). Movement proteins are targeted preferentially to calreticulin-labeled foci within the youngest cross walls, where they assemble into tubules. During cell division, they colocalize in the cell plate with KNOLLE, a cytokinesis-specific syntaxin. {ECO:0000250}.; SUBCELLULAR LOCATION: [Protein 2A]: Host cytoplasm. Host nucleus. Note=Cytoplasmic early in infection. Later in infection, it becomes progressively concentrated around the nucleus, where it forms large aggregates.; SUBCELLULAR LOCATION: [Coat protein]: Virion {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages in vivo by the P1 encoded 3C-like protease yield mature proteins. {ECO:0000269|PubMed:2374004, ECO:0000269|PubMed:8376967}. |
| Signal Peptide | |
| Structure 3D | Electron microscopy (1); X-ray crystallography (3) |
| Cross Reference PDB | 2Y26; 4V5T; 4V5W; 5FOJ; |
| Mapped Pubmed ID | 21352920; 21625570; 32371486; |
| Motif | |
| Gene Encoded By | |
| Mass | 122,707 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |