| IED ID | IndEnz0002000908 |
| Enzyme Type ID | protease000908 |
| Protein Name |
RNA2 polyprotein Genome polyprotein M M RNA polyprotein Middle component RNA polyprotein P2 Cleaved into: VP58; Movement protein MP ; Large capsid protein LCP 42k coat protein Coat protein VP42 L subunit Large coat protein ; Small capsid protein SCP 22k coat protein Coat protein VP22 S subunit |
| Gene Name | |
| Organism | Squash mosaic virus (strain melon) (SqMV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Picornavirales Secoviridae Comovirinae Comovirus Squash mosaic virus Squash mosaic virus (strain melon) (SqMV) |
| Enzyme Sequence | MWHFCEQVYECFEGYHRDYSVQTVPVEYLASHYIVNKFRPDPLAVLWLFCLGIWWEIIQILHHLFQYKEPALFVGSCQNLAAFLEKKYSMEVIQKEGLAASALKDKERLTEKAVVNQPLSNLIPHSNKMYERSKSLLSGLKRGLIKQKEIAFDKLMGGSTIDFQHIPTGTLTPGENKVLDIPIVPQHLLTSTNITDYHQANKKNANGATALHVGAIEVIMDCFTSPDSNICGGMLLVDTAHLNPDNAIRSVFVAPFIGGRPIRVLLFPDTLVEIAPNMNSRFKLLCTTSNGDVAPDFNLAMVKVNVAGCAVSLTRTYTPTAYLEQELIKEKGAIVQYLNRHTFSMHRNNQMTKEEMQKQRLSFRLESALTLQEKHPLHATFCKSTNFVYKIGGDAKEGSNGNLTVNESQLSSHSPSAHVLHKHNNSGDNEVEFSEIGVVVPGAGRTKAYGQNELDLAQLSLDDTSSLRGTALQTKLATSRIILSKTMVGNTVLREDLLATFLQDSNERAAIDLIRTHVIRGKIRCVASINVPENTGCALAICFNSGITGAADTDIYTTSSQDAIVWNPACEKAVELTFNPNPCGDAWNFVFLQQTKAHFAVQCVTGWTTTPLTDLALVLTWHIDRSLCVPKTLTISSAHASFPINRWMGKLSFPQGPARVLKRMPLAIGGGAGTKDAILMNMPNAVISLHRYFRGDFVFEITKMSSPYIKATIAFFIAFGDITEEMTNLESFPHKLVQFAEIQGRTTITFTQSEFLTAWSTQVLSTVNPQKDGCPHLYALLHDSATSTIEGNFVIGVKLLDIRNYRAYGHNPGFEGARLLGISGQSTMVQQLGTYNPIWMVRTPLESTAQQNFASFTADLMESTISGDSTGNWNITVYPSPIANLLKVAAWKKGTIRFQLICRGAAVKQSDWAASARIDLINNLSNKALPARSWYITKPRGGDIEFDLEIAGPNNGFEMANSSWAFQTTWYLEIAIDNPKQFTLFELNACLMEDFEVAGNTLNPPILLS |
| Enzyme Length | 1009 |
| Uniprot Accession Number | P36341 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: [VP58]: Responsible for viral RNA2 accumulation. May function by recruiting the RNA1-encoded polyprotein that contains the replication protein to RNA2 and enable its replication. {ECO:0000250|UniProtKB:P23009}.; FUNCTION: [Movement protein]: Transports the viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids. Binds to GTP and to single-stranded RNA and single-stranded DNA in a non-sequence-specific manner. {ECO:0000250|UniProtKB:P03599}.; FUNCTION: [Large capsid protein]: Together with the small capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms. The capsid is formed from 60 copies each of the large and the small capsid protein. The large capsid protein interacts with the viral RNA. {ECO:0000250|UniProtKB:P03599}.; FUNCTION: [Small capsid protein]: Together with the large capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms. The capsid is formed from 60 copies each of the large and the small capsid protein. The small capsid protein forms the turrets at the fivefold axes of the viral particle. {ECO:0000250|UniProtKB:P03599}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (5); Natural variant (3); Region (1); Site (3) |
| Keywords | Capsid protein;DNA-binding;Direct protein sequencing;GTP-binding;Host cell junction;Nucleotide-binding;RNA-binding;Suppressor of RNA silencing;T=3 icosahedral capsid protein;Transport;Viral movement protein;Virion |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Movement protein]: Host cell junction, host plasmodesma {ECO:0000250|UniProtKB:P03599}. Note=Assembles in tubules that are embedded within modified plasmodesmata. {ECO:0000250|UniProtKB:P03599}.; SUBCELLULAR LOCATION: [Large capsid protein]: Virion {ECO:0000250|UniProtKB:P03599}.; SUBCELLULAR LOCATION: [Small capsid protein]: Virion {ECO:0000250|UniProtKB:P03599}. |
| Modified Residue | |
| Post Translational Modification | PTM: [RNA2 polyprotein]: Specific enzymatic cleavages by picornain 3C-like protease in vivo yield mature proteins. {ECO:0000305|PubMed:8503782}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 111,788 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |