| IED ID | IndEnz0002000913 |
| Enzyme Type ID | protease000913 |
| Protein Name |
Retrovirus-related Pol polyprotein from transposon 412 Includes: Protease EC 3.4.23.- ; Reverse transcriptase EC 2.7.7.49 ; Endonuclease |
| Gene Name | POL ORF3 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | QQSEQLQQKQCQCTSNPRTGQLATAFRYSVEEDRRVYTINYNLNIFSTFIHAKTGVKLVFLLDTGADISILKENSDKFSNIQITNKINIQGIGQQKIQSRGQTFIEIQTGKYVIPHDFHLVDKNFPIPCDGIIGIDFIKKYNCQIDLNQEEDWFIIRPNNLKFPIYIPIAYSSGINTTLLPARSQVVRRLIVSSKDDNILIPNQEIQTGIYVANTIATSSNTFVRILNTTDSDQLVNMDTLKYEPLSNYNVVQANSEHRNKTVLSQLKKNFPELFKSQLENICSEYIDIFALESEPITVNNLYKQQLRLKDDEPVYTKNYRSPHSQVEEIQAQVQKLIKDKIVEPSVSQYNSPLLLVPKKSSPNSDKKKWRLVIDYRQINKKLLADKFPLPRIDDILDQLGRAKYFSCLDLMSGFHQIELDEGSRDITSFSTSNGSYRFTRLPFGLKIAPNSFQRMMTIAFSGIEPSQAFLYMDDLIVIGCSEKHMLKNLTEVFGKCREYNLKLHPEKCSFFMHEVTFLGHKCTDKGILPDDKKYDVIQNYPVPHDADSARRFVAFCNYYRRFIKNFADYSRHITRLCKKNVPFEWTDECQKAFIHLKSQLINPTLLQYPDFSKEFCITTDASKQACGAVLTQNHNGHQLPVAYASRAFTKGESNKSTTEQELAAIHWAIIHFRPYIYGKHFTVKTDHRPLTYLFSMVNPSSKLTRIRLELEEYNFTVEYLKGKDNHVADALSRITIKELKDITGNILKVTTRFQSRQKSCAGKEQLDLQKQTKEIASEPNVYEVITNDEVRKVVTLQLNDSICLFKHGKKIIARYDVGDLYTNGILDLDQFLQRLELQAGIYDISQIKMAPWKKIFEHVSIDKFKNMGNKILKNLKVALLNPVTQINNEKEKEAILSTLHDDPIQGGHTGITKTLAKVKRHYYWKNMSKYIKEYVRKCQKCQKAKTTKHTKTPMTITETPEHAFDRVVVDTIGPLPKSENGNEYAVTLICDLTKYLVAIPIANKSAKTVAKAIFESFILKYGPMKTFITDMGTEYKNSIITDLCKYLKIKNITSTAHHHQTVGVVERSHRTLNEYIRSYISTDKTDWDVWLQYFVYCFNTTQSMVHNYCPYELVFGRTSNLPKHFNKLHSIEPIYNIDDYAKESKYRLEVAYARARKLLEAHKEKNKENYDLKVKDIELEVGDKVLLRNEVGHKLDFKYTGPYKIESIGDNNNITLLTNKNKKQIVHKDRLKKFHS |
| Enzyme Length | 1237 |
| Uniprot Accession Number | P10394 |
| Absorption | |
| Active Site | ACT_SITE 63; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU10094 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-ProRule:PRU00405}; |
| DNA Binding | |
| EC Number | 3.4.23.-; 2.7.7.49 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (3) |
| Keywords | Aspartyl protease;Endonuclease;Hydrolase;Nuclease;Nucleotidyltransferase;Protease;RNA-directed DNA polymerase;Transferase;Transposable element |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16882972; 23559253; |
| Motif | |
| Gene Encoded By | |
| Mass | 143,042 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:22508 |
| Cross Reference Brenda |