| IED ID | IndEnz0002000916 |
| Enzyme Type ID | protease000916 |
| Protein Name |
Polyprotein p69 ORFA polyprotein Cleaved into: Papain-like protease p29 EC 3.4.22.- ; p40 protein |
| Gene Name | |
| Organism | Cryphonectria hypovirus 1 (strain EP713) (CHV-1/EP713) (Chestnut blight fungus hypovirulence-associated virus) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Duplopiviricetes Durnavirales Hypoviridae Hypovirus Cryphonectria hypovirus 1 Cryphonectria hypovirus 1 (strain EP713) (CHV-1/EP713) (Chestnut blight fungus hypovirulence-associated virus) |
| Enzyme Sequence | MAQLRKPSQSLVLSESVDPTTVDPFVSVRTEEVVPAGCITLWEYRDSCGDVPGPLSHGDLRRLRTPDGVCKCQVHFELPTVLKSGSTGTVPEHPAVLAAFIGRPRRCSLEQRTKELDSRFLQLVHGGLPARPSYMIARPPRPVRGLCSSRNGSLAQFGQGYCYLSAIVDSARWRVARTTGWCVRVADYLRLLQWVGRRSFGSFQIEKSAVDHVYHVVVDAEYQSEQDGALFYQAILGLAEKDPLARIGGRLNPLAAEFAPGSALRVEPVTPQVTRRKGSTRMTGRDPTIVSVGKVGMAITSIQDALVATELRNVNFGRRDTEAECRRLWARYEVNDYFRRHKAELLKFDARLRSRMAKKPASSRARPSDAKIQCIGWRDRHLLPQRLAGLSKQGRSLVWSRFATSNIRRKTPPCVVNPSADPVVHNWKDSAALAVKKIAEARRRQEIRAAAYAERAKARGQTNVVASISEAIETTLRRNKTRFALDGLHLAASAIVTTRLRSWNQEEIRAGREFRKSTTSWIWRHVPSSIQDALNLTSVRDKLDPGRAFGYVQAAVAQGMSDFRRAKRALAIVAKPVIRNIRDPYEHGFVKRDGKLRHSRDAFNKKLRTKAVAATKVHKIKF |
| Enzyme Length | 622 |
| Uniprot Accession Number | P10941 |
| Absorption | |
| Active Site | ACT_SITE 162; /note=For papain-like protease p29 activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01225; ACT_SITE 215; /note=For papain-like protease p29 activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01225 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: P40 protein is involved in reduction of conidiation of the host. Not necessary for replication. Also involved in reduction of orange pigmentation of the host.; FUNCTION: [Papain-like protease p29]: Cysteine protease of the peptidase family C7 that contributes to hypovirulence-associated traits like the reduction in conidiation and laccase activity, but not to virulence attenuation. Acts as suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of viral defense that limits the accumulation of viral RNAs. Enhances viral dsRNA accumulation and virus transmission. Also involved in the reduction in orange pigmentation of the host, an effect independent of the intrinsic protease activity. {ECO:0000255|PROSITE-ProRule:PRU01225}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (2); Domain (1); Frameshift (2); Mutagenesis (14); Region (1); Site (1) |
| Keywords | Hydrolase;Protease;Reference proteome;Suppressor of RNA silencing;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: [Papain-like protease p29]: Autocatalytically processed. {ECO:0000269|PubMed:1996319}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 69,688 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |