| IED ID | IndEnz0002000918 |
| Enzyme Type ID | protease000918 |
| Protein Name |
ORFB polyprotein Cleaved into: Papain-like protease p48 EC 3.4.22.- ; Putative RNA-directed RNA polymerase/helicase EC 2.7.7.48 EC 3.6.4.13 |
| Gene Name | |
| Organism | Cryphonectria hypovirus 1 (strain EP713) (CHV-1/EP713) (Chestnut blight fungus hypovirulence-associated virus) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Duplopiviricetes Durnavirales Hypoviridae Hypovirus Cryphonectria hypovirus 1 Cryphonectria hypovirus 1 (strain EP713) (CHV-1/EP713) (Chestnut blight fungus hypovirulence-associated virus) |
| Enzyme Sequence | MYKEAERPIEVWRTQVMDGPTWTALSESCRDRLFFASGEGGEHMTLDIIQPDSYTKIRLFRSGRFEVSVDGKSFGQGGNRYRFVFRYDSLLSTPFGYPAEDKEIALQDYNHKQLLGEMFLKLPDSYVDGRPIAEAFFRYVDDLKWDVGVFRDRRSLTELHLPASSGLTTAQVSVAKLEWPPLPIIQAQPTILAGIIDNFKICFPVNGKWIYGQGLSWTRYDGDASVPTSLLSNRQHARFWNEKDIPTGLKLSKEGFIKLWAQKSRKWQDHMARSIGLSHEAAVELVRATRVNEAKPHLVPMEEAKEAPRQQLVPRRSTFVDNHEEEVEIDTLRVPVEEGRCFELLFNNQVTPAIFDKKPLLKDVLGVFEENVCTMDSLEISHSDQCVHIVAGETFRNYDEIKAVLEVILENEPDILVGAEEGSVADYVKAGKHFLFENHQWVRNGLKLAKGLAEPGQRAKDNTNPSTPRPIEDADYIHPFDNGQPLPGRSDQWVSGFEVTRLRHHDEMPHIRSVRNTGIHGLPGDFLSNYPRLPTPVFHRLRDLWDDVIGILMKLEFGDNCSPVLNVTANADWVRSETTINFISDQPGKAQSRPREDGGFDILVPCRGIATRSIRLLPLFIRLPNRFRAVALLNGRQSDYDNYGWPVFNPVIPLPQMDSFYVEAVAAGRSMYPPGFLLGRYDALEYLVHTATVYGAEEAFLLPFTHHVRVYPPPRPGREIPFGSWCKNYKFEAERFWYDADWKLRVHETNHDFDRLIEITKTCRRNPPEENLQAKLEDTARKVCSVWQYNIMIASSVAFLVPLYFTLYVPYLQFYLHVDPGDYILLPPVLWLVWTNLCYGYACDAWCRLFFFVEEAGKKELVHSSEEFSSDPSSTLLIPTMGTRGDHVPPRFFANMAVLAGVKTHLLKLQTATYGDLENLKKGKLGSLLPGYLQNHYSVLRGYKAAFTPHVELDMPNATSYNLAPPRSYINKIRYLTDENRSGASMIDRAVTWFAEELADTFWPDWQIGCLRGCNLPRSADGVSLITKQPNLKTGKIGWLHGSADPAVVPKDIRDKYPLVPNGDHNEIFRHYDKIYMPGGAGAVQTAIACGCEVVVTDVNLDRDYHTMPTQKDFHQPSILPYFAWLWRQGFDVKLPRVLLVIGWLKFHYSIRYKHLEFAADFVIRAGLFWWYGCLHLLPFMAAAIMAPRFVKKYLVGMAWLTEPGLLMLKALWRFPIFMVTPRWMLPFIVTVSVYNWWWPLSQDGLNYASKRFELIFEPVTRGKHTFSYPFGHWCLRDTNSMIVYEGKFVNPSETSIGSPFKLSKSVRPVRPGAVFHLVPFHVQKLLDSMDEAPLPYSANHNCTTVILKGIMYRSALGFVFAYMVSWAVYLVLRPPQAAATVYHWVYPERSWDTSRLYHLLLGFAAGGTVPMEVIDEEHVEEKPSVAGQSEPAAEIDNDKISDYDQEWWGSQDSIDTVVNDLCYLLSFLKDTAIPEEVKLDVVELAYTQLVQDEKERIPEPKGTKILDMPNWKPGNWAKLIDETHRVLSQFTQYTPRVLNELVVWLKGLGENLYRVAEPILMLLVRAMRAAKSVSDRATRSVYHCLCHWLDVMYGGSAPTRVKTVWGLTGLVASGMTSQKAILAQNIAMMEYQGRGNFLDDYDNFVSNIKEPGKGLPGINTIGGPQRRPIRYKNPVMSHQAAEICGLKPGEYEVDDRYQERINDYLAEGIPQAVDGVLFGDRNPDRIARSISRYEPEYSGCSPEDKALVEDTARAMFEQWPEVFADRDIMLPKGVELYIKEKYSAGTPFISSFYKSRKALKQAGVMDVIRKNALECISTGKYPTQFYHAFAKSQAVPGQPLLAPRMKDLRTVVSEDLSAYMVDQIFQIEANKRITWETYGAGSGMPLSQSMARIWDELHDLRKREGGQFIIADATAYDSNCKPALFHGAGKLVELGFQNHPSGKGRQFAQVVQCKFEAMQNAWVMGITEPSYTALTFHVPDVAVRHELESKYPAHFATFSELLAHNNVNVTEWKRLSWEERKACARDMQAVPGKVFLTNDPALRLQGSSWQGSFTTEPKRDEFRKYQTYFYDSKAAMREDIKRIVFANREVISNVHHKNRGGGTGQSATSWDNTATFKLGVISAWARATGKPPKDFFCSNRLYNTSDDTVWWSKDLLSSAEVDRFKQAAADFGILLEIGSTKKITEVEYLSKLPRRPTAEDSADYRAWRQGRIENMRSSGRFSEEQLLSIEREQLPQFLMVQNPTAILMRRTAFRYYQSSPSKFLYTSCERGAGHALVTAFQPALYKRFAIEYAEDLNRLCKEHHINQRYELVSQQDRMKMQVINVNPNWKRNFKLSPRQEAFLRWIRQAKFPSYRQVLDIHLRIRDPDPSAHDRFIAKLDRAWRNPDEGIRDIVDGVYRYTDMIPEEFKRFMPSTDMLYAENPWHTHNQYVEKFIYLKLLETTTVDELTFAQFDAVAKESPYGICMNTIKFWEDLRDPDYLKDLLASEAMIDKVRIYQGMTVIISAMYFAMHWVELFIQSLFLIGPLYNLFMWSFWGLSKVYGLANTFYWHGKARSSREISSILPRDPYMWSKRFVSTMADFIPERFALGIVPVTLVLDGLAEIIEVLFGRMWRLFANLKSVGTDFSDARSGKSLNVPSNPWAAYAHTYATKAIEHGHVTVAAKTASGKSTFFPAAVWAERRNIGIKKLWIVMPRKILRDNWEIPFDIRSQIVKRGKTLDPSADIYVTTYGHFRTRIGGLVPRDNLVFFDEFHEMDGFMLQDVEDWKGPTIFMSATPVALHGMAGIPFLEPTLPKRFNLTVYKVDSDDVLEMWNRARNQFADQPELLARPMIIVPTYNELKKTIAGLENLDRSITWHEVSSNSPLVPKTGGLVCTPYVQTGIDIKPAPSILIDSGRDVIVHKGRLVTPHPYTDEKTNEQRVNRVGRTMDGVVIQPQLAGTGNPPVKYPSGIFFSSELVAGQYKVPRLTKVNGCVHPELPYMSIKYTSELSDPAKAREEEQSVTKSLLFIHLMALAGVRQSEWALRYNRYFELHLPFGEDEDHLERILTSGKLRYANHIPVDMAMQLLGNGHVTWGIGGVPTITRPRYPCDGMWVEDPSSRKSYAHKVLLHQREHAEIGMWQAQVNELRAQNLALQSQLRSACTRRSTAGRILRHTRPPDIPVCG |
| Enzyme Length | 3165 |
| Uniprot Accession Number | Q04350 |
| Absorption | |
| Active Site | ACT_SITE 341; /note=For papain-like protease p48 activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01223; ACT_SITE 388; /note=For papain-like protease p48 activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01223 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; |
| DNA Binding | |
| EC Number | 3.4.22.-; 2.7.7.48; 3.6.4.13 |
| Enzyme Function | FUNCTION: Papain-like protease p48 is a cysteine protease of the peptidase family C8. {ECO:0000255|PROSITE-ProRule:PRU01223}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 2664..2671; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00541 |
| Features | Active site (2); Chain (2); Domain (2); Motif (1); Mutagenesis (31); Nucleotide binding (1); Region (2); Site (1); Transmembrane (9) |
| Keywords | ATP-binding;Direct protein sequencing;Helicase;Host membrane;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Putative RNA-directed RNA polymerase/helicase]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Papain-like protease p48 is autocatalytically processed. The putative RNA-directed RNA polymerase/helicase may be further processed. {ECO:0000269|PubMed:1918054}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 2751..2754; /note=DEFH box |
| Gene Encoded By | |
| Mass | 361,718 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248; RHEA:13065 |
| Cross Reference Brenda |