IED ID | IndEnz0002000919 |
Enzyme Type ID | protease000919 |
Protein Name |
ORFB polyprotein Cleaved into: Papain-like protease p48 EC 3.4.22.- ; Putative RNA-directed RNA polymerase/helicase EC 2.7.7.48 EC 3.6.4.13 |
Gene Name | |
Organism | Cryphonectria hypovirus 1 (strain Euro7) (CHV-1/Euro7) (Chestnut blight fungus hypovirulence-associated virus) |
Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Duplopiviricetes Durnavirales Hypoviridae Hypovirus Cryphonectria hypovirus 1 Cryphonectria hypovirus 1 (strain Euro7) (CHV-1/Euro7) (Chestnut blight fungus hypovirulence-associated virus) |
Enzyme Sequence | MYKEAERPIEVWRTQVMDGPTWTALSEPCRNRLFFASGKGGEHLTLDVIQPDSYTKIRLFRSGRFEVSVDGKSFGQGGNRYRFVFRYDSLLSTPFGYPAEDKEMALQGYNHEQLLGEMFLKLPDSYVDGRPIAEAFFRYVDDLKWDVGVFRDRRSLTELHLPASSGLTTEQARVAKLEWPPLPIIQAQPTILAGIIDNFKICFPVNGKWVYGQGLSWTRYDGDASVPISLLTNRQHARFWNDKDVPTGLKLSKEGFIKLWAQKSRKWQDHMARAIGLSHAATAELVRATKVNEAKPHLVPMEEAKEAPRQQLVPRRSTFVDSHEKGVEVDPLRLPTEEGRCFELLFNNQVTPAIFDKKPLLRDVLAVFKENVCTMDSLEISHSDRCVHIVTGETFRNYKEIKAVLEVIIWNDPNILVGAEEGSIADYVKAGKHFLFENHQWVRNGLKLAKGLAEPGQLAKDNTNPSTPKPIETTDYIHPFDNGQPLPGRSDQWVSGFEVTRLRHHEEMPHIRNVRNTGIHGLPGDFLSNYPRLPTPVFHRLRDLWYDVIGILMKLEFGDNRSPVLNVTANADWVRSETTVNFISDQPGKARSRPRKDGGFDILVPCRGIATRSIRLLPLFIRLPPRFKAVALLNGRQSDYDNYGWPVFNPVIPLPQIDSFYVEAVAAGRSMYPPGFLLDRYDALGFLIHTATVYGAEEAFLLPFTHHARVYPPPRPGREIAFGSWCKNYKFTAERYWYDADWKLRVHETNHDFDRLIEITKTCRRNPPEENLQARLKDTAREVCSIWQYNIMIASSVAFLIPLFYTLYVPYLQFYLHVDPGDYMLLPPVLWLVWTNLCYGYACDAWCRLFFFVEEAGKKELVHSSEEFSSDPSSTLLIPTMGTRGDHVPPRFFANMAVLAGVKTHLLKLQTATYGDLENLKKGKLGSLLPGYLQNHYSVLRGYKAVFTPHVELDMPNATSYNLAPPRSYINKIRYLTDENRSGASIVDRAVTWFAEELADTFWPDWQIGCLRGCNLPRSADGVSLITKRPNLKTGKIGWLHGSADPAVVPKDIRDRYPLVPNGDHNEIFRHYDKIYMPGGAGAVQTAIACGCEVVVTDVNLDRDYHTMPTQKDFHQPSILPYFAWLWRQGFDVKLPRVLLVVGWLKFHYSIRYKHLEFAADFVIRAGLFWWYGCLHLLPFMAAAIMTPRFVKKYLVSMAWLTEPGLLMLKALWRFPIFMVTPRWMLPFIVTVSAYNWWWPLSQDGLNYASKRFELIFEPVARGKYTFSYPFGHWCLRDTNSMIIYEGKFVDSSETSIGSPFKLSKSVRPVRPGAVFHLVPFHIQKLLDSMDEEPLPYSANHNCTTVILKGIMYRSALGFVFAYAVSWAVYLVLRPPQAAATVYHWMYPERSWDTSRLYHLLGFAAGGTVPMEVIDEEPIEEKPSDAGRSEPIPDNDKQEESDYDQEWWGSQDSIDTVSNDLCYLLSFLKDTAIPEEVKLDVIELAYTQFVRNEKGRIPEPKETRILVMPNWKPDNWARLIDETHRVLSQFTHYTPRVLNELVVWLKGLGENLYRVAEPILMLLVRAMRAAKSVSDRATRSIYHCLCHWLDVMYGGSAPTRVKTVWGLTGLIASGMTSQKAILAQNIAMMEYQGRGNFLDDYDNFVSNIKEPGKGLPGINTIGGPQRRPIRYKNPVMSHQAAEICGLKPGEYEVDEKYQERINDYLAEGIPQAVDGVLFGDRNPDRIARSINRYEPEYSGCSPEDKALVEDTARAMFEQWPEVFADRDIMLPKGVELYIKEKYSAGTPFISSFYKSRKALKQAGVMDVIRKNALECIKTGKYPTQFYHAFAKSQAVPGQPLLAPRMKDLRTVVSEDLSAYMVDQIFQIEANKRITWETYGAGSGMPLSQSMARIWDELHDLRKREGGQFIIADATAYDSNCKPVLFHGAGKLVELGFQNHPSGKGRQFAQVVQCKFEAMQNAWVMGITEPSYSALTFHVPDAEVRRDLESKFPRHFVTFSELLEHNNMNVTEWKRLTWEEQKACARDMQSVPGKVFLTNDPALRLQGSSWQGSFTTEPKRDEFRKYQTYFCNSKEAMKEDIKRIVFANREVISNVHHKNRGGGTGQSATSWDNTATFKLGVISAWARATGKLPKDFFCSNRLYNTSDDTVWWSKDLLSSAEVDRFKQAAADFGILLEIGSTKKITEVEYLSKLPRRPTAEDSADYRTWRQGRIENMRSSGRFTEEQMLSIEREQLPQFLMVQNPTAILMRRTAFRYYQSSPSKFLYTSCERGAGHALVTAFQPALYKRFAIEYAEDLNRLCKEHHINQRYELVSQQDRIKMQVINVNPNWKQGFRLSPRQEAFLRWIRQAKFPSYRQVLDIHLRTKDPDPSAHDRFIAKLDRAWRNPDEGIRDMVDGVYRYTDLIPEEFKRFMPSTDMLYAENPWHTHNQYVEKFIYLKLLETTTVDELTFAQFDAVAKESPYGICMNTIKFWEDLRDPDYLKDLLASEAMIDKVRIYQGMTVIISAMYFAMHWVELFVQSLFLIGPLYNLFMWSFWGLSKVYGLANTFYWHGKARSSREISSIMPRDPYMWSKRFVSTMADFIPERFALGLVPATLILDGLAEIIEVLFGRMWRMFANLKSVGTDFGDARSGKSLNVPSNPWAAYAHTYATKAIEHGHVTVAAKTASGKSTFFPAAVWAERRNIGVKKLWIVMPRKILRDNWEIPFDIRSQIVKRGKTLDPTADIYITTYGHFRTRIGGLVPRDNLVFFDEFHEMDGFMLQDVEEWKGPTIFMSATPVALHGMADIPFLEPTLPKRFNLTVYKVDSDDVLEMWNRARNQFADQPALLARPMIIVPTYNELKKTIAGLENLDRSVTWHEVSSNSPFVPKTGGLVCTPYVQTGIDIKPAPSILIDSGRDVVVHKGRLITPHPYTDEKTNEQRVNRVGRTMDGVVIQPQLAGTGDPPVKYPSGIFFSSRLVAGQYRVPRLTEVDGCVHPELPYISIKYTSELSNPVEAKKEEQNVRKSLLFIHLMALAGVRQSEWALRYNRYFELHLPFGEDEDHLQRILEQGKLRYAHHIPVDMAMQLLGNGHVTWGIGGVPTITRPRYPCDGMWVEDPSSRKSYVHKVLLHQREHAEIGMWQAQVNELKAQKLALQSQLRSVCTRRSTASRILRHIRPPDIPVCG |
Enzyme Length | 3164 |
Uniprot Accession Number | Q9YTU2 |
Absorption | |
Active Site | ACT_SITE 341; /note=For papain-like protease p48 activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01223; ACT_SITE 388; /note=For papain-like protease p48 activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01223 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; |
DNA Binding | |
EC Number | 3.4.22.-; 2.7.7.48; 3.6.4.13 |
Enzyme Function | FUNCTION: Papain-like protease p48 is a cysteine protease of the peptidase family C8. {ECO:0000255|PROSITE-ProRule:PRU01223}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | NP_BIND 2663..2670; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00541 |
Features | Active site (2); Chain (2); Compositional bias (1); Domain (2); Motif (1); Nucleotide binding (1); Region (2); Site (1); Transmembrane (9) |
Keywords | ATP-binding;Helicase;Host membrane;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Transmembrane;Transmembrane helix |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: [Putative RNA-directed RNA polymerase/helicase]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
Modified Residue | |
Post Translational Modification | PTM: Papain-like protease p48 is autocatalytically processed. The putative RNA-directed RNA polymerase/helicase is probably further processed (By similarity). {ECO:0000250}. |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | MOTIF 2750..2753; /note=DEFH box |
Gene Encoded By | |
Mass | 362,598 |
Kinetics | |
Metal Binding | |
Rhea ID | RHEA:21248; RHEA:13065 |
Cross Reference Brenda |