| IED ID | IndEnz0002000957 |
| Enzyme Type ID | protease000957 |
| Protein Name |
Genome polyprotein Cleaved into: Nuclear inclusion protein A NI-A NIA EC 3.4.22.44 49 kDa proteinase 49 kDa-Pro ; Nuclear inclusion protein B NI-B NIB EC 2.7.7.48 RNA-directed RNA polymerase ; Capsid protein CP Coat protein Fragment |
| Gene Name | |
| Organism | Watermelon mosaic virus II (isolate USA) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Stelpaviricetes Patatavirales Potyviridae Potyvirus Watermelon mosaic virus Watermelon mosaic virus II (isolate USA) |
| Enzyme Sequence | LSVTRKQCLELGMVLSLSPMGTYLEGTMGCLQLKHGHGGFVIHNTTQLRIHFIQGKDAILIRMPKIFLRLQSATSLDNQNVRNEFAWLEQTFKRRAYGQQSQSSSIILPEGKGSFWIHWITTQDGFCGLPLVSVNDGHVVGIHGLTSNDSEKNFFVPFTDGFEKEYLDNADNLSWDKHWFWEPSKIAWGPLNLVEEQPKEEFKISKLVSDLFGNTVAVQSRKERWVLDAMEGNLVACGQADSALVTKHVVKGKCPYFAQYLSLHDGAXQFFEPLMGAYQPSRLNKDAFKKDFFKYNKPVVLNEVDFNAFEKAVEGVITMMVDFEFAECLFVTDPDEIYGSLNMKAAVGAQYKGKKQDYFSGMDSFDKERLLYLSCERLFNGEKGIWNGSLKAELRPIEKVQANKTRTFTAAPLDTLLGAKVCVDDFNNQFYSFNLKCPWTVGMTKFYGGWDKLMRSLPDGWTYCHADGSQFDSSLTPLLLNAVLSIRCCFMEDWWVGREMLENLYAEIVYTPILAPDGTIFKKFRGNNSGQPSTVVDNTLMVVIAMYYSCCKQGWSEEDIERRLVFFANGDDIILAVKDEDVWLYDTLSASFAELGLNYNFDERTKKREELWFMSHQAMLVDGIYIPKLEPERIVSILEWDRSKELMHRTEAICAAMIEAWGYTELLQEIRKFYLWLLSKDEFKELAASGKAPYIAETALRKLYTDVNTQPSELQRYLEVLDFNHIDGCCESVSLQSGKETVENLDAGKESKKDASDKGNKPQNSQVGQGSKEPTKTGTVSKDVNVGSKGKEVPRLQKITKKMNLPTVGGKIILSLDHLLEYKPSQVDLFNTRATKTQFESWYSAVKVEYDLNDEQMGVIMNGFMVWCIDNGTSPDVNGVWVMMDGEEQVEYPLKPIVENAKPTLRQIMHHFSDAAEAYIEMRNSESPYMPRYGLLRNLRDRELARYAFDFYEVTSKTPNRAREAIAQMKAAALAGVNSRLFGLDGNISTNSENTGRHTARDVNQNMHTLLGMGPPQ |
| Enzyme Length | 1017 |
| Uniprot Accession Number | P18478 |
| Absorption | |
| Active Site | ACT_SITE 57; /note=For nuclear inclusion protein A activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00766; ACT_SITE 127; /note=For nuclear inclusion protein A activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00766 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.; EC=3.4.22.44; CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 3.4.22.44; 2.7.7.48 |
| Enzyme Function | FUNCTION: [Nuclear inclusion protein B]: An RNA-dependent RNA polymerase that plays an essential role in the virus replication.; FUNCTION: [Nuclear inclusion protein A]: Has RNA-binding and proteolytic activities. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (4); Compositional bias (2); Domain (2); Non-terminal residue (1); Region (1); Site (2) |
| Keywords | Capsid protein;Genetically modified food;Hydrolase;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Thiol protease;Transferase;Viral RNA replication;Virion |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Capsid protein]: Virion {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Genome polyprotein of potyviruses undergoes post-translational proteolytic processing by the main proteinase NIa-pro resulting in the production of at least ten individual proteins. The P1 proteinase and the HC-pro cleave only their respective C-termini autocatalytically. 6K1 is essential for proper proteolytic separation of P3 from CI (By similarity). {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 115,489 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |