| IED ID | IndEnz0002000959 |
| Enzyme Type ID | protease000959 |
| Protein Name |
Genome polyprotein Cleaved into: RNA replication protein; Capsid protein CP1 CP1 Coat protein Includes: RNA-directed RNA polymerase EC 2.7.7.48 ; Helicase EC 3.6.4.13 ; Methyltransferase EC 2.1.1.- |
| Gene Name | ORF1 |
| Organism | Maize rayado fino virus (isolate Costa Rica/Guapiles) (MRFV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Kitrinoviricota Alsuviricetes Tymovirales Tymoviridae Marafivirus Maize rayado fino virus Maize rayado fino virus (isolate Costa Rica/Guapiles) (MRFV) |
| Enzyme Sequence | MSSFLRGGHLLSGVESLTPTTHRDTITAPIVESLATPLRRSLERYPWSIPKEFHSFLHTCGVDISGFGHAAHPHPVHKTIETHLLLDVWPNYARGPSDVMFIKPEKFAKLQSRQPNFAHLINYRLVPKDTTRYPSTSTNLPDCETVFMHDALMYYTPGQIADLFFLCPQLQKIYASVVVPAESSFTHLSLHPEIYRFRFQGSDLVYEPEGNPAANYTQPRSALDWLQTTGFTVGHEFFSVTLLDSFGPVHSLLIQRGRPPVFQAEDIASFRVPDAVALPAPASLHQDLRHRLVPRKVYDALFNYVRAVRLRVTDPAGFVRTQVGKPEYSWVTSSAWDNLQHFALQTAAVRPNTSHPLFQSPFARLSHWLRTHTWALWCLASPSASVSAWATASALGRLLPLHTDRLRLFGFDIIGRRFWPRLPFHGPEPRFLWETHPACRPPVLFADSAFECQILAGLANRCSPSPFWSRLFPTASPPSWVAYSALALAAVPLAALALRWFYGPDSPQALHDQYHATFHPDPWTLDLPRRLRRFERESFMRTGSAPLPQSLPPPEGSLLPVEPPPVPSDPEPALEPSPPAASVPAPAPALASEPPPSPESVAPSRRRRRARRAAARAPSPSPALLGADLRFGDLPPVSAWDSDPEISKLGESTQGTVFAVTPGPRAPEPDTARLDADPSASGPVMEFRELQKGAYIEPTGAFLTRARNSVSSSIPYPTRAACLLVAVSQATGLPTRTLWAALCANLPDSVLDDGSLATLGLTTDHFAVLARIFSLRCRFVSEHGDVELGLHDATSRFTIRHTPGHFELVADNFSLPALVGASSVPGADLAEACKRFVAPDRTVLPFRDVHIHRTDVRRAKNLISNMKNGFDGVMAQANPLDPKSARERFLMLDSCLDIAAPRRVRLIHIAGFAGCGKSWPISHLLRTPAFRVFKLAVPTTELRDEWKALMDPRDQDKWRFGTWESSLLKTARVLVIDEVYKMPRGYLDLAIHADAAIQFVILLGDPIQGEYHSTHPSSSNARLSPEHRYLRPYVDFYCFWSRRIPQNVARVLDVPTTSTEMGFARYSQQFPFSGKILISARDSAKSLADCGYHAVTIASSQGSTIAGPAYVHLDNHSRRLSHQHSLVAITRSKSGIVFTGDKAAADGTSSANLLFSAVLLDRRLSVRSLFSALLPCCPFVTEPPTSRAVLLRGAGYGIARPLRARDAPPLGPDYVGDVILDSSAPILGDGSANAPQVSTHFLPETRRPLHFDIPSARHQVADHPLAPDHSACAIEPVYPGESFESLASLFLPPTDAESKETYFRGEMSNQFPHLDKPFELGAQTSSLLAPLHNSKHDPTLLPASIGKRLRFRHSEAPYVIAPRDEILGSLLYEAWCRAYHRSPRDVEPFDPDLYAECINLNEFAQLSSKTQATIMANANRSDPDWRWSAVRIFAKTQHKVNEGSLFGSWKACQTLALMHDAVVLLLGPVKKYQRFFDQRDRPSTLYVHAGHTPFEMADWCRAHLTPAVKLANDYTAFDQSQHGEAVVFERYKMNRLSIPAELVDLHVYLKTNVSTQFGPLTCMRLTGEPGTYDDNTDYNIAVLHLEYAVGSTPLMVSGDDSLLDSEPPVRDQWSAIAPMLALTFKKERGRYATFCGYYVGFTGAVRSPPALFAKLMIAVDDGSISDKLIAYLTEFTVGHSSGDAFWTILPVEAVPYQSACFDFFCRRAPAQAKVMLRLGEAPESLLSLAFEGLKWASHSVYALMNSSHRRQLLHSSRRPRSLPEDPEVSQLQGELLHQFQSLHLPLRGGHMPNPLAAPFRLLQQSSSLGPTYAVAPIARAPQVPPPSMADNATQVGPVPPRDDRVDRQPPLPDPPRVLETAPSHFLDLPFQWKVTDFTGYAAYHGTDDLVASAVLTTLCAPYRHAELLYVEISVAPCPPSFSKPIMFTVVWTPATLSPRDGKETDYYGGRQITVGGPVMLSSTTAVPADLARMNPFIKSSVSYNDTPRWTMSVPAVTGGDTKIPLATAFVRGIVRVRAPSGAATPSA |
| Enzyme Length | 2027 |
| Uniprot Accession Number | Q91TW9 |
| Absorption | |
| Active Site | ACT_SITE 722; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01074; ACT_SITE 805; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01074 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; |
| DNA Binding | |
| EC Number | 2.7.7.48; 3.6.4.13; 2.1.1.- |
| Enzyme Function | FUNCTION: RNA replication protein replicates the viral genomic RNA. The central part of this protein possibly functions as an ATP-binding helicase and/or methyltransferase (Probable). {ECO:0000305|PubMed:3721810}.; FUNCTION: Capsid protein CP1 and CP2 assemble to form an icosahedral capsid, about 30 nm in diameter, and consisting of capsid proteins CP1 and CP2 in a 1:3 ratio. The capsid encapsulates the single-stranded RNA genome. While CP1 is produced as a C-terminal fusion of the replication protein, CP2 may be expressed from a 3'-co-terminal subgenomic RNA. {ECO:0000269|PubMed:3721810}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Chain (3); Compositional bias (1); Domain (5); Region (3) |
| Keywords | 3D-structure;ATP-binding;Alternative promoter usage;Capsid protein;Hydrolase;Methyltransferase;Multifunctional enzyme;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Viral RNA replication;Virion |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Capsid protein CP1]: Virion {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 7MIA; 7MIC; |
| Mapped Pubmed ID | 34265303; |
| Motif | |
| Gene Encoded By | |
| Mass | 223,738 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248; RHEA:13065 |
| Cross Reference Brenda |