| IED ID |
IndEnz0002001081 |
| Enzyme Type ID |
protease001081 |
| Protein Name |
Urokinase-type plasminogen activator
U-plasminogen activator
uPA
EC 3.4.21.73
Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B
|
| Gene Name |
PLAU |
| Organism |
Homo sapiens (Human) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Metazoa
Eumetazoa
Bilateria
Deuterostomia
Chordata
Craniata
Vertebrata
Gnathostomata (jawed vertebrates)
Teleostomi
Euteleostomi
Sarcopterygii
Dipnotetrapodomorpha
Tetrapoda
Amniota
Mammalia
Theria
Eutheria
Boreoeutheria
Euarchontoglires
Primates
Haplorrhini
Simiiformes
Catarrhini
Hominoidea (apes)
Hominidae (great apes)
Homininae
Homo
Homo sapiens (Human)
|
| Enzyme Sequence |
MRALLARLLLCVLVVSDSKGSNELHQVPSNCDCLNGGTCVSNKYFSNIHWCNCPKKFGGQHCEIDKSKTCYEGNGHFYRGKASTDTMGRPCLPWNSATVLQQTYHAHRSDALQLGLGKHNYCRNPDNRRRPWCYVQVGLKLLVQECMVHDCADGKKPSSPPEELKFQCGQKTLRPRFKIIGGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLISPCWVISATHCFIDYPKKEDYIVYLGRSRLNSNTQGEMKFEVENLILHKDYSADTLAHHNDIALLKIRSKEGRCAQPSRTIQTICLPSMYNDPQFGTSCEITGFGKENSTDYLYPEQLKMTVVKLISHRECQQPHYYGSEVTTKMLCAADPQWKTDSCQGDSGGPLVCSLQGRMTLTGIVSWGRGCALKDKPGVYTRVSHFLPWIRSHTKEENGLAL |
| Enzyme Length |
431 |
| Uniprot Accession Number |
P00749 |
| Absorption |
|
| Active Site |
ACT_SITE 224; /note=Charge relay system; ACT_SITE 275; /note=Charge relay system; ACT_SITE 376; /note=Charge relay system |
| Activity Regulation |
ACTIVITY REGULATION: Inhibited by SERPINA5. {ECO:0000269|PubMed:14696115, ECO:0000269|PubMed:3501295}. |
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
CATALYTIC ACTIVITY: Reaction=Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.; EC=3.4.21.73; |
| DNA Binding |
|
| EC Number |
3.4.21.73 |
| Enzyme Function |
FUNCTION: Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Active site (3); Alternative sequence (1); Beta strand (36); Chain (4); Disulfide bond (12); Domain (3); Glycosylation (2); Helix (10); Modified residue (2); Mutagenesis (2); Natural variant (4); Region (2); Sequence conflict (4); Signal peptide (1); Site (1); Turn (4) |
| Keywords |
3D-structure;Alternative splicing;Blood coagulation;Direct protein sequencing;Disulfide bond;EGF-like domain;Fibrinolysis;Glycoprotein;Hemostasis;Hydrolase;Kringle;Pharmaceutical;Phosphoprotein;Plasminogen activation;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With |
Q9UKQ2; P05067; Q03405-1 |
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24434139}. |
| Modified Residue |
MOD_RES 158; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:9151681; MOD_RES 323; /note=Phosphoserine; /evidence=ECO:0000269|PubMed:9151681 |
| Post Translational Modification |
PTM: Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. {ECO:0000269|PubMed:9151681}.; PTM: Produced as an inactive single-chain protein (pro-uPA or sc-uPA), is processed into the active disulfide-linked two-chain form of PLAU/uPA by a proteolytic event mediated, at least, by TMPRSS4. {ECO:0000269|PubMed:24434139}. |
| Signal Peptide |
SIGNAL 1..20; /evidence="ECO:0000269|PubMed:2023947, ECO:0000269|PubMed:6754569" |
| Structure 3D |
NMR spectroscopy (2); X-ray crystallography (147) |
| Cross Reference PDB |
1C5W;
1C5X;
1C5Y;
1C5Z;
1EJN;
1F5K;
1F5L;
1F92;
1FV9;
1GI7;
1GI8;
1GI9;
1GJ7;
1GJ8;
1GJ9;
1GJA;
1GJB;
1GJC;
1GJD;
1KDU;
1LMW;
1O3P;
1O5A;
1O5B;
1O5C;
1OWD;
1OWE;
1OWH;
1OWI;
1OWJ;
1OWK;
1SC8;
1SQA;
1SQO;
1SQT;
1U6Q;
1URK;
1VJ9;
1VJA;
1W0Z;
1W10;
1W11;
1W12;
1W13;
1W14;
2FD6;
2I9A;
2I9B;
2NWN;
2O8T;
2O8U;
2O8W;
2R2W;
2VIN;
2VIO;
2VIP;
2VIQ;
2VIV;
2VIW;
2VNT;
3BT1;
3BT2;
3IG6;
3KGP;
3KHV;
3KID;
3M61;
3MHW;
3MWI;
3OX7;
3OY5;
3OY6;
3PB1;
3QN7;
3U73;
4DVA;
4DW2;
4FU7;
4FU8;
4FU9;
4FUB;
4FUC;
4FUD;
4FUE;
4FUF;
4FUG;
4FUH;
4FUI;
4FUJ;
4GLY;
4H42;
4JK5;
4JK6;
4K24;
4MNV;
4MNW;
4MNX;
4MNY;
4OS1;
4OS2;
4OS4;
4OS5;
4OS6;
4OS7;
4X0W;
4X1N;
4X1P;
4X1Q;
4X1R;
4X1S;
4XSK;
4ZHL;
4ZHM;
4ZKN;
4ZKO;
4ZKR;
4ZKS;
5HGG;
5WXF;
5WXO;
5WXP;
5WXQ;
5WXR;
5WXS;
5WXT;
5XG4;
5YC6;
5YC7;
5Z1C;
5ZA7;
5ZA8;
5ZA9;
5ZAE;
5ZAF;
5ZAG;
5ZAH;
5ZAJ;
5ZC5;
6AG2;
6AG3;
6AG7;
6AG9;
6JYP;
6JYQ;
6L04;
6L05;
6NMB;
6XVD;
7DZD;
|
| Mapped Pubmed ID |
10779411;
10926521;
11052791;
11292354;
11731301;
11751384;
11805108;
11827968;
11858184;
11876553;
11880102;
11906036;
11928806;
11928807;
11928809;
11928812;
11928816;
11928818;
11930938;
11956327;
12008951;
12023847;
12041678;
12084931;
12115506;
12149463;
12152683;
12161038;
12167592;
12174885;
12218297;
12236587;
12353084;
12377770;
12393547;
12430175;
12477728;
12529357;
12556966;
12574820;
12576295;
12642587;
12684001;
12684636;
12704669;
12708473;
12719778;
12719789;
12736046;
12742021;
12743986;
12754271;
12764090;
12773490;
12832381;
12881310;
12883725;
12898287;
12926141;
12930304;
12941043;
12960238;
14507113;
14517288;
14531820;
14534291;
14597629;
14644829;
14681909;
14704150;
14711304;
14744782;
14747469;
14769799;
14983219;
14983227;
15067375;
15083890;
15149645;
15149885;
15150279;
15217951;
15231498;
15243126;
15262029;
15326109;
15356878;
15371454;
15375521;
15494526;
15515049;
15522303;
15558716;
15582418;
15615772;
15616835;
15629242;
15646834;
15653692;
15728176;
15750768;
15831288;
15849510;
15854129;
15868845;
15880258;
15881651;
15885322;
15927351;
15937335;
15944400;
15996887;
16008557;
16077991;
16102112;
16113755;
16236890;
16267271;
16385451;
16459332;
16461772;
16504015;
16509805;
16525631;
16547007;
16601839;
16604464;
16632475;
16685436;
16685447;
16761300;
16794252;
16806969;
16807059;
16825285;
16825821;
16836076;
16855359;
16858643;
16908524;
16949925;
16967469;
16979660;
17013094;
17029796;
17080225;
17083312;
17159381;
17174555;
17187779;
17192053;
17203175;
17222367;
17233842;
17240317;
17258797;
17264329;
17299802;
17299841;
17327908;
17331567;
17344041;
17355798;
17363771;
17447747;
17458906;
17523079;
17532189;
17545513;
17548050;
17549401;
17559626;
17581316;
17601350;
17611702;
17634019;
17649957;
17661167;
17681345;
17692534;
17709546;
17877801;
17880283;
17905583;
17921261;
17934860;
17949787;
17974965;
17986506;
17994220;
18048089;
18053985;
18076013;
18163548;
18177545;
18193533;
18196271;
18202522;
18208536;
18237545;
18239069;
18240004;
18257282;
1829461;
18331597;
18337556;
18355442;
18362146;
18376307;
18376415;
18390474;
18436804;
18439850;
1846368;
18466392;
18478929;
18491991;
18511987;
18519237;
18570153;
18599586;
18600101;
18691743;
18714186;
18788551;
18824154;
18940913;
18949356;
18954937;
18977241;
18988861;
19020551;
19029443;
19088796;
19097143;
19101688;
19102416;
19115132;
19117638;
19123477;
19132247;
19133257;
19147749;
19157142;
19176991;
19177205;
19232508;
19272641;
19298527;
19394555;
19403319;
19436306;
19475533;
19497963;
19513384;
19526059;
19536843;
19546228;
19616049;
19701247;
19703758;
19703768;
19723042;
19729488;
19730683;
19735728;
19740518;
19776673;
19826838;
19845941;
19889475;
19903858;
19913121;
20015050;
20036101;
20039121;
20043086;
20052287;
20097831;
20198323;
20204407;
20337595;
20369484;
20403162;
20445443;
20452482;
20518747;
20565774;
20584616;
20628086;
20628624;
20645833;
20651370;
20673868;
20693403;
20708412;
20740581;
20805979;
20931209;
20937265;
21071923;
21076612;
21104439;
21174062;
21181094;
21199867;
21219832;
21242962;
21316840;
21426933;
21567400;
2156852;
21574189;
21627387;
2166055;
21669207;
21670079;
21709151;
21711960;
21744990;
21761413;
21798735;
21802428;
21819230;
21833477;
21858104;
21913037;
21965736;
21988832;
21989445;
22004682;
22102275;
22166006;
22199285;
22280367;
22285761;
22291969;
22294827;
22304751;
22354580;
22449964;
22467324;
22470492;
22575880;
22684547;
22702340;
22707264;
22797075;
22797919;
22906080;
22927998;
22986534;
22994764;
23016918;
23059760;
23064953;
23322578;
23350235;
23392805;
23441133;
23532423;
23536772;
23560397;
23597113;
23615713;
23621237;
23628797;
23650620;
23661254;
23812296;
23813610;
23828687;
23848154;
23864708;
23897640;
23898067;
23900236;
23978400;
23985164;
24023701;
24057344;
24111848;
24217605;
24340014;
24453110;
24465541;
24479343;
24481457;
24529115;
24610082;
24616477;
24626561;
24648800;
24702774;
24756697;
24807734;
24822208;
24877154;
24952395;
25082749;
25085839;
25215905;
2521625;
25244504;
25323935;
25343607;
25371209;
25394558;
2544876;
25544710;
25545505;
25554417;
25555911;
25641046;
25706093;
25744057;
25962429;
25979345;
25993872;
26027394;
26033187;
26050228;
26166362;
26275878;
26281711;
26317203;
26504024;
26643086;
26707376;
26782341;
26846181;
26877613;
26978018;
27226628;
27391159;
27502396;
27599390;
27783304;
27975070;
27986809;
28104444;
28278500;
28301587;
28340565;
28385810;
28429105;
28454121;
28506312;
28643198;
28643742;
28644504;
28685212;
28954632;
2935528;
29760745;
29890880;
29959058;
30130401;
3023326;
30241478;
30646864;
30783124;
30814058;
3090045;
30937621;
31217736;
31270884;
31307406;
31317530;
31317567;
31990070;
32019421;
33146708;
33221341;
33804289;
33804930;
33896830;
33998175;
34244472;
34716325;
8161544;
9765311;
|
| Motif |
|
| Gene Encoded By |
|
| Mass |
48,523 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
|
| Cross Reference Brenda |
3.4.21.73; |