| IED ID | IndEnz0002001082 |
| Enzyme Type ID | protease001082 |
| Protein Name |
Urokinase-type plasminogen activator U-plasminogen activator uPA EC 3.4.21.73 Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B |
| Gene Name | Plau |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MKVWLASLFLCALVVKNSEGGSVLGAPDESNCGCQNGGVCVSYKYFSRIRRCSCPRKFQGEHCEIDASKTCYHGNGDSYRGKANTDTKGRPCLAWNAPAVLQKPYNAHRPDAISLGLGKHNYCRNPDNQKRPWCYVQIGLRQFVQECMVHDCSLSKKPSSSVDQQGFQCGQKALRPRFKIVGGEFTEVENQPWFAAIYQKNKGGSPPSFKCGGSLISPCWVASAAHCFIQLPKKENYVVYLGQSKESSYNPGEMKFEVEQLILHEYYREDSLAYHNDIALLKIRTSTGQCAQPSRSIQTICLPPRFTDAPFGSDCEITGFGKESESDYLYPKNLKMSVVKLVSHEQCMQPHYYGSEINYKMLCAADPEWKTDSCKGDSGGPLICNIEGRPTLSGIVSWGRGCAEKNKPGVYTRVSHFLDWIQSHIGEEKGLAF |
| Enzyme Length | 433 |
| Uniprot Accession Number | P06869 |
| Absorption | |
| Active Site | ACT_SITE 226; /note=Charge relay system; ACT_SITE 277; /note=Charge relay system; ACT_SITE 378; /note=Charge relay system |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by SERPINA5. {ECO:0000250|UniProtKB:P00749}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.; EC=3.4.21.73; |
| DNA Binding | |
| EC Number | 3.4.21.73 |
| Enzyme Function | FUNCTION: Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. {ECO:0000250|UniProtKB:P00749}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (27); Chain (4); Disulfide bond (12); Domain (3); Helix (9); Modified residue (1); Region (2); Signal peptide (1); Turn (3) |
| Keywords | 3D-structure;Disulfide bond;EGF-like domain;Hydrolase;Kringle;Phosphoprotein;Plasminogen activation;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | Q9Z0K7 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P00749}. |
| Modified Residue | MOD_RES 159; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:P00749 |
| Post Translational Modification | PTM: Produced as an inactive single-chain protein (pro-uPA or sc-uPA), is processed into the active disulfide-linked two-chain form of PLAU/uPA by a proteolytic event mediated, at least, by TMPRSS4. {ECO:0000250|UniProtKB:P00749}. |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (8) |
| Cross Reference PDB | 3LAQ; 5LHN; 5LHP; 5LHQ; 5LHR; 5LHS; 6A8G; 6A8N; |
| Mapped Pubmed ID | 10022836; 10072260; 10090151; 10191100; 10318667; 10335479; 10417613; 10487767; 10502816; 10716993; 10725249; 10858190; 10880388; 10891445; 10903758; 10961882; 11003846; 11025665; 11059781; 11106569; 11154109; 11157667; 11213252; 11238040; 11238111; 11306532; 11404420; 11441114; 11676474; 11760891; 11777975; 11818362; 11827968; 11858492; 11891190; 11907112; 11920190; 11929773; 11983447; 12171938; 12183060; 12209601; 12237117; 12356640; 12376355; 12413883; 12431907; 12493778; 12591758; 12600880; 12657615; 12692088; 12759445; 12854826; 12875972; 12890575; 12911578; 1346779; 1353738; 1371498; 14617754; 14645112; 14681479; 14688127; 14747469; 15096455; 15161662; 15240745; 15254013; 15284078; 15297377; 15308506; 1535334; 15472905; 15528930; 15615772; 15631996; 15711734; 15716324; 15743790; 15795331; 15841177; 16125860; 16127152; 16148155; 16224065; 16474180; 1655359; 16554301; 16601674; 16601837; 16604464; 16615898; 1673105; 16757700; 16763560; 1676977; 16787960; 1686571; 16880329; 16887829; 16908524; 16971476; 17015742; 17114493; 17203182; 17234207; 17356128; 17382917; 17434507; 17574041; 17598016; 1762914; 17634529; 17652428; 17675481; 17785520; 17893885; 1794049; 1794058; 18042398; 18084301; 18164548; 18178858; 18193533; 18279442; 18318660; 18337556; 18606671; 18667463; 18691680; 18784372; 18948573; 18957535; 19210287; 19286661; 19459212; 19461880; 19690163; 1971251; 19812386; 19863693; 20026272; 20097831; 20123981; 20177776; 20196869; 20204677; 20351234; 20466854; 20805979; 20856796; 20886179; 20973954; 21075828; 21273553; 21301782; 21326869; 21403399; 21536666; 21540184; 21573723; 21617202; 21677750; 21704601; 21709151; 21798735; 21802414; 21868698; 21931850; 21969013; 21998707; 22177333; 22280367; 22293605; 22318286; 22451728; 22560297; 22563169; 22912336; 22950516; 23060546; 23169000; 23183001; 23236466; 23340304; 23665346; 2377456; 23853591; 24023701; 24120085; 24194600; 24269585; 24348274; 24616477; 24682410; 24918201; 24929950; 25061113; 25168896; 25172911; 25339736; 25405459; 25447049; 25454795; 25616415; 25673638; 25753270; 25893677; 25960543; 25979345; 26253597; 26265072; 26393505; 26527290; 26577922; 26647393; 26846181; 26863767; 26930384; 27140400; 27151212; 27262672; 27480124; 27986809; 2821634; 28298247; 28336948; 28385810; 28611361; 28931568; 28972182; 29107070; 29420634; 29720403; 30300579; 30382077; 30605632; 30707839; 30718289; 30836238; 31251468; 31362033; 31705517; 31756973; 31758376; 31819012; 32187956; 32332118; 32360936; 34236404; 34450502; 34675506; 34716325; 7588068; 7743917; 7743938; 7760840; 7818535; 7858255; 7894166; 7979162; 8012109; 8043949; 8075499; 8104765; 8119140; 8133887; 8179996; 8188294; 8251184; 8382771; 8453613; 8507976; 8560424; 8618918; 8621764; 8647936; 8660977; 8679252; 8717349; 8758932; 8798151; 8801257; 8812413; 8833158; 8903730; 8938449; 8944773; 8959348; 8979118; 9006351; 9079647; 9351457; 9398846; 9425170; 9438420; 9576763; 9600959; 9649555; 9680394; 9699672; 9701458; 9727060; 9733098; 9927592; 9989802; |
| Motif | |
| Gene Encoded By | |
| Mass | 48,268 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |