Detail Information for IndEnz0002001115
IED ID IndEnz0002001115
Enzyme Type ID protease001115
Protein Name Peptidoglycan D,D-transpeptidase MrdA
EC 3.4.16.4
Penicillin-binding protein 2
PBP-2
Gene Name mrdA pbpA SL1344_1845
Organism Salmonella typhimurium (strain SL1344)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella typhimurium Salmonella typhimurium (strain SL1344)
Enzyme Sequence MTFKDFDAEEKLFLRRVIVAFGVVVVCFGILIFNLYNLQIRQHHYYTTRSNENDIKMLPVAPTRGIIYDRNGIPLVRNVTWYDIAVTPYKIADMDALLKQLTPIVDLSPDDISDFRRALKSSSRYRPVVLKNALTDVEIARFAVNQFHFNGVTINSYQDRQYPYGAELAHVLGYVSKINDNDLKALDKKGLAENYAADHNIGKQGIERYYENDLHGKTGYQEVEVDNHGRIVRLLKDVPPIAGKNIHLTLDLHLQEYIESLLAGQRAAVLVEDPHDGSVLAMVSMPSYDPNPFVKGISYQDYGKLLHDKNLPLINRVTQGLYPPASTVKPYMAMSALLCGIITPQTTFFGAPTWTLPGTQRHYRDWKKTGHGMLDVTKAIEESADTFFYQVAYMMGIDRIDTMLSQFGYGKPTGIDLNEEYDGLLPSRAWKQRVHKKAWYQGDTISVGIGQGYWIATPIQMVKAMVALINNGKVIAPHLLLNEESGKTVVPYRPSGTPAQIADPASPYWGLVRQAMYGMANAPNGTGYKFFHTAPYGIAAKSGTSQVFSLKENQTYNAKMIPIRLRDHVFYTAFAPYKNPKVAIALILENGGSDGVTAAPIMRKILDHLFDPQADTTQPDQAP
Enzyme Length 623
Uniprot Accession Number E1WGF1
Absorption
Active Site ACT_SITE 326; /note=Acyl-ester intermediate; /evidence=ECO:0000255|HAMAP-Rule:MF_02081
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.; EC=3.4.16.4; Evidence={ECO:0000255|HAMAP-Rule:MF_02081};
DNA Binding
EC Number 3.4.16.4
Enzyme Function FUNCTION: Catalyzes cross-linking of the peptidoglycan cell wall. {ECO:0000255|HAMAP-Rule:MF_02081}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis. {ECO:0000255|HAMAP-Rule:MF_02081}.
nucleotide Binding
Features Active site (1); Chain (1); Sequence conflict (2); Transmembrane (1)
Keywords Carboxypeptidase;Cell inner membrane;Cell membrane;Cell shape;Cell wall biogenesis/degradation;Hydrolase;Membrane;Peptidoglycan synthesis;Protease;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-Rule:MF_02081}; Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_02081}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 69,833
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda