Detail Information for IndEnz0002001131
IED ID IndEnz0002001131
Enzyme Type ID protease001131
Protein Name Neutral protease 2 homolog PADG_00776
EC 3.4.24.39
Deuterolysin PADG_00776
Gene Name PADG_00776
Organism Paracoccidioides brasiliensis (strain Pb18)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Onygenales Onygenales incertae sedis Paracoccidioides Paracoccidioides brasiliensis Paracoccidioides brasiliensis (strain Pb18)
Enzyme Sequence MRRVSGILAVAAFTISAFAGVIQPVAKDARDSAELDVKLTQVDGTVIKAVVTNNGDKDLNILNLNFFRDTAPVKKVSIYSQGVEVPFGGIRVRHKTSDLSSDVITYLAPGESFEDEFDVAITSDLSQGGPVVLQTQGYVPTTDTGGKTLSGVVRYKSNKLEIDVDGTTAAKSFAAMNQFVKIAKLSSCEGSQGDDTRRALRDCASLSTLAAAQAWAGGPKMLEYFKANDDATRKLVADRFTAVALESSNLTGGSTTYYCRDPYNICTNNIIAYTIPAENLISNCPIYYTEFDNVNRKCHGQDRVTTSLHEFTHASSVFSPGTKDIAYGYNACILLSTRDALNNADTFALFAQSINAGC
Enzyme Length 358
Uniprot Accession Number C1G1N6
Absorption
Active Site ACT_SITE 310; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Preferential cleavage of bonds with hydrophobic residues in P1'. Also 3-Asn-|-Gln-4 and 8-Gly-|-Ser-9 bonds in insulin B chain.; EC=3.4.24.39;
DNA Binding
EC Number 3.4.24.39
Enzyme Function FUNCTION: Secreted metalloproteinase that allows assimilation of proteinaceous substrates. Shows high activities on basic nuclear substrates such as histone and protamine (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Disulfide bond (2); Erroneous gene model prediction (1); Glycosylation (1); Metal binding (3); Propeptide (1); Signal peptide (1)
Keywords Cleavage on pair of basic residues;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..19; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 38,509
Kinetics
Metal Binding METAL 309; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 313; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 324; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095
Rhea ID
Cross Reference Brenda 3.4.24.39;