| IED ID | IndEnz0002001161 |
| Enzyme Type ID | protease001161 |
| Protein Name |
PAN2-PAN3 deadenylation complex catalytic subunit PAN2 EC 3.1.13.4 PAB1P-dependent poly A -specific ribonuclease Poly A -nuclease deadenylation complex subunit 2 PAN deadenylation complex subunit 2 |
| Gene Name | PAN2 CNBD1450 |
| Organism | Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) (Filobasidiella neoformans) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Tremellomycetes Tremellales (jelly fungi) Cryptococcaceae Cryptococcus Cryptococcus neoformans species complex Cryptococcus neoformans (Filobasidiella neoformans) Cryptococcus neoformans var. neoformans Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) (Filobasidiella neoformans) |
| Enzyme Sequence | MNYNPLHLLPLPPTALDPKPIPTSLTLDPFSDVLWVGASSGIVSALCSPLTLARNVHFPAHGCKIGGGGFSAQGVSAVREVRVTDRDVWTLTEGGIGGRKRGGAPKWIVSDVTRSLRTMSPNPTNSHELITGGSGSLLLANTARGEVVRSIENSSPVVKLAPLHRTVLAAGLSGQVTVLDPRTGFKAAQNISPVQAHTGGLSGADVQGNIVATWGWTHMQGHPLPDPLIRLYDVRALRPLPPISFSSGPAFVLLHPSSSSHIVVSSQQGMLQTIDMSLGPSATVFQQLDVSSYITSMALSSRGDYLAFGDGDGQLHVWTTNETGENAAVDENGSIVLPPFNGYDGVKPEWPDQVDPLPTIAWEESTPLNLVGMPYYNEALLSQFPSECYATSTSPLFNPPATIPQPVLSSMKMVDFVGYAPNPKELRGKRYVLRAVPGAEGRARGKGRRDSGPRFRSEKDKKGTYKDKEEIEEELNDGEVPKYYRKVEIKYSKFGIEDFDFEFYNRTNYSGLETDILNSYTNSLLQALHYTLPLRAIATAHICVDCKKEHCLLCEAGFLFRMLEDAKGRNCQASNFSRAFSATSQAYALGLMDENTNSKSTAPYGSLIQNFNRWLLSTFSTESIVDGETFHLRPFPQKTSGLDGLSMNDGPSAIDQVLGVKIKTTNTCRHCGFVSSRDSTLHVVDLVYPKKMNPRLSFSDILRSSLIRDSTTKAICSSCKAFAPLDSRRSLSPSSGHPLPPVLSVNAMVTNSDVYGFWKDKKDVKEKDGVRRFLPKRVTIREVGKLENGQEEGVKYSIRSMVVQIQESPDAVAHLVSFVKMPSKDGSSAWIMFNDFLVRPVSEDEVLSFPDQWKVPAVIILERENAEELLNLEVLPKELDREILFKDVSIAWNRKQDMIKHKILQREEMPKRGTLVAIDAEFVALQQEEMEFRSDGTKNILRPSHMSLARVSVLRGEGEMEGKPFIDDYIHTSEAVVDYLTEFSGIKAGDLDPNNSPHTLVPLKVAYKKLRLLVDLGCIFVGHGLSKDFRTINIFVPPEQVMDTVLIYTLPGSQRKLSLRFLAWYLLHQDIQTNSHDSIEDAHFALLLCKLWMDYASESEEAFEIVMEDIFAEGKKLAFKPPSSAGNMMAEQQLSPVSFPPLSNGDQTVARAVVKSRMATPPPPTKLGLPQWASQNSPSPLRR |
| Enzyme Length | 1183 |
| Uniprot Accession Number | P0CQ09 |
| Absorption | |
| Active Site | |
| Activity Regulation | ACTIVITY REGULATION: Positively regulated by the regulatory subunit PAN3. {ECO:0000255|HAMAP-Rule:MF_03182}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Exonucleolytic cleavage of poly(A) to 5'-AMP.; EC=3.1.13.4; Evidence={ECO:0000255|HAMAP-Rule:MF_03182}; |
| DNA Binding | |
| EC Number | 3.1.13.4 |
| Enzyme Function | FUNCTION: Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein PAB1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails. {ECO:0000255|HAMAP-Rule:MF_03182}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (2); Domain (2); Metal binding (4); Region (3); Repeat (2) |
| Keywords | Cytoplasm;Exonuclease;Hydrolase;Metal-binding;Nuclease;Repeat;WD repeat;mRNA processing |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03182}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 130,525 |
| Kinetics | |
| Metal Binding | METAL 919; /note=Divalent metal cation; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03182; METAL 921; /note=Divalent metal cation; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03182; METAL 1028; /note=Divalent metal cation; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03182; METAL 1081; /note=Divalent metal cation; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03182 |
| Rhea ID | |
| Cross Reference Brenda |