Detail Information for IndEnz0002001186
IED ID IndEnz0002001186
Enzyme Type ID protease001186
Protein Name Replicase polyprotein P2AB
Cleaved into: N-terminal protein; Serine protease
EC 3.4.21.-
; VPg; RNA-directed RNA polymerase
EC 2.7.7.48
RdRp
Gene Name ORF2A-2B
Organism Southern bean mosaic virus (isolate Bean/United States/Arkansas) (SBMV)
Taxonomic Lineage Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Sobelivirales Solemoviridae Sobemovirus Southern bean mosaic virus Southern bean mosaic virus (isolate Bean/United States/Arkansas) (SBMV)
Enzyme Sequence MYHPGRSPSFLITLANVICAAILFDIHTGGYQPGSLIPIVAWMTPFVTLLWLSASFATYLYKYVRTRLLPEEKVARVYYTAQSAPYFDPALGVMMQFAPSHGGASIEVQVNPSWISLLGGSLKINGDDASNESAVLGSFYSSVKPGDEPASLVAIKSGPQTIGFGCRTKIDGDDCLFTANHVWNNSMRPTALAKRGKQVAIEDWETPLSCDHKMLDFVVVRVPKHVWSKLGVKATQLVCPSDKDAVTCYGGSSSDNLLSGTGVCSKVDFSWKLTHSCPTAAGWSGTPIYSSRGVVGMHVGFEDIGKLNRGVNAFYVSNYLLRSQETLPPELSVIEIPFEDVETRSYEFIEVEIKGRGKAKLGKREFAWIPESGKYWADDDDDSLPPPPKVVDGKMVWSSAQETVAEPLNLPAGGRVKALAALSQLAGYDFKEGEAASTRGMPLRFVGQSACKFRELCRKDTPDEVLRATRVFPELSDFSWPERGSKAELHSLLLQAGKFNPTGIPRNLEGACQNLLERYPASKSCYCLRGEAWSFDAVYEEVCKKAQSAEINEKASPGVPLSRLASTNKDLLKRHLELVALCVTERLFLLSEAEDLLDESPVDLVRRGLCDPVRLFVKQEPHASRKVREGRFRLISSVSLVDQLVERMLFGPQNQLEIAEWEHIPSKPGMGLSLRQQAKSLFDDLRVKHSRCPAAEADISGFDWSVQDWELWADVEMRIVLGGFGHKLAKAAQNRFSCFMNSVFQLSDGTLIEQQLPGIMKSGSYCTSSTNSRIRCLMAELIGSPWCIAMGDDSVEGWVDGAKDKYMRLGHTCKDYKPCATTISGRLYEVEFCSHVIREDRCWLASWPKTLFKYLSEGKWFFEDLERDVSSSPHWPRIRHYVVGNTPSPHKTNLQNQSPRYGEEVDKTTVNQGYSEHSGSPGHSIEEAQEPEAAPFCCEAASVYPGWGVHGPYCSGDYGSLT
Enzyme Length 962
Uniprot Accession Number O72157
Absorption
Active Site ACT_SITE 181; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216; ACT_SITE 216; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216; ACT_SITE 284; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48;
DNA Binding
EC Number 3.4.21.-; 2.7.7.48
Enzyme Function FUNCTION: [Serine protease]: Responsible for cleavages of polyprotein P2A and replicase polyprotein P2AB.; FUNCTION: [VPg]: Covalently attached to the 5' extremity of the genomic and subgenomic RNAs. It may serve as a primer for the replicase.; FUNCTION: [RNA-directed RNA polymerase]: Replicates the viral genome. {ECO:0000305}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (5); Domain (2); Site (3); Transmembrane (3)
Keywords Covalent protein-RNA linkage;Direct protein sequencing;Host membrane;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Ribosomal frameshifting;Serine protease;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: [N-terminal protein]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: [Replicase polyprotein P2AB]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
Modified Residue
Post Translational Modification PTM: The polyprotein is proteolytically cleaved into several chains by the viral protease.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 106,566
Kinetics
Metal Binding
Rhea ID RHEA:21248
Cross Reference Brenda