| IED ID | IndEnz0002001187 |
| Enzyme Type ID | protease001187 |
| Protein Name |
Replicase polyprotein P2AB Cleaved into: N-terminal protein; Serine protease EC 3.4.21.- ; VPg; RNA-directed RNA polymerase EC 2.7.7.48 RdRp |
| Gene Name | ORF2A-2B |
| Organism | Southern cowpea mosaic virus (SCPMV) (Southern bean mosaic virus (strain cowpea)) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Sobelivirales Solemoviridae Sobemovirus Southern cowpea mosaic virus (SCPMV) (Southern bean mosaic virus (strain cowpea)) |
| Enzyme Sequence | MYRPSCLSYVLLVANMWSFAVCANAFIYGSYDPSHNIPIVALMTLCATGLWLSTSVVSFGIRYVRVRVSPEKTQNRTIYVSSGLPHFDPVYGVVKKCEPMGGGPAIELQVNPSWIHLLPTSPAINKVEVGQESAILGSTYSVVETGGEPKSLVAVKSGDSTLGFGARVYHEGMDVLMVPHHVWYNDKPHTALAKNGRSVDTEDWEVEAACADPRIDFVLVKVPTAVWAKLAVRSTKVLAPVHGTAVQTFGGQDSKQLFSGLGKAKALDNAWEFTHTAPTAKGWSGTPLYTRDGIVGMHTGYVDIGTSNRAINMHFIMSCLVSKMETLPPELGYREISLEDVGLRSFEFLEVEIENRGKVKLGKREFAWVPKGKAWADMLDDDDLPLPPKMVNGNLVWADAQESFDGALPLNLLAGGRTQCLAAQIELGDYKFSCGPTHETGGMPFRNCGSSTCKFREVSRKPVADAVTAATKVFPELSELGWPERGSGAEIGSLLLQAGKFVPTKAPSNLEQAYNNLLSRYPRSKPLACFRQGTWSFDAIFEQVVSKATSAEINQKASPGVPLSRLATTNKDLMAQHMQFVAACVTGRVPLLASFEDIHALSPTEMVEMGLCDPVRLFVKQEPHPSRKLKEGRYRLISSVSIVDQLVERMLFGAQNELEIAEWQSIPSKPGMGLSVIHQADAIFRDLRVKHTVCPAAEADISGFDWSVQDWELWADVEMRIVLGSFPPMMARAARNRFSCFMNSVLQLSNGQLLQQELPGIMKSGSYCTSSTNSRIRCLMAELIGSPWCIAMGDDSVEGFVEGAREKYAGLGHLCKDYKPCATTPTGQLYAVEFCSHVIKRNKAFLTSWPKTLYRFLSTPRETLEDLERELASSPMWHKIQSYVRSIPSPDKTARDKSICNGYPLDQEAISTSYSEYSSKSASAEATREAACCAGAQAYPSWGIHGPYCSGDHGEA |
| Enzyme Length | 956 |
| Uniprot Accession Number | P21405 |
| Absorption | |
| Active Site | ACT_SITE 181; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216; ACT_SITE 216; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216; ACT_SITE 284; /note=For protease activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU01216 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 3.4.21.-; 2.7.7.48 |
| Enzyme Function | FUNCTION: [Serine protease]: Responsible for cleavage of polyprotein P2A and replicase polyprotein P2AB.; FUNCTION: [VPg]: Covalently attached to the 5' extremity of the genomic and subgenomic RNAs. It may serve as a primer for the replicase.; FUNCTION: [RNA-directed RNA polymerase]: Replicates the viral genome. {ECO:0000255|PROSITE-ProRule:PRU00539}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (5); Domain (2); Site (3); Transmembrane (3) |
| Keywords | Covalent protein-RNA linkage;Host membrane;Hydrolase;Membrane;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Ribosomal frameshifting;Serine protease;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [N-terminal protein]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: [Replicase polyprotein P2AB]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: The polyprotein is proteolytically cleaved into several chains by the viral protease. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 104,849 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |