| IED ID | IndEnz0002001194 |
| Enzyme Type ID | protease001194 |
| Protein Name |
Replicase polyprotein 1ab Cleaved into: Leader protease L-Pro EC 3.4.22.- Papain-like cysteine proteinase PCP ; Methyltransferase/helicase/RNA-directed RNA polymerase EC 2.1.1.- EC 2.7.7.48 EC 3.6.4.13 |
| Gene Name | 1a-1b |
| Organism | Beet yellows virus (isolate Ukraine) (BYV) (Sugar beet yellows virus) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Kitrinoviricota Alsuviricetes Martellivirales Closteroviridae Closterovirus Beet yellows virus Beet yellows virus (isolate Ukraine) (BYV) (Sugar beet yellows virus) |
| Enzyme Sequence | MAFLNVSAVPSCAFAPAFAPHAGASPIVPDSFPCVPRYSDDISHFRLTLSLDFSVPRPLSLNARVHLSASTDNPLPSLPLGFHAETFVLELNGSSAPFSIPSRHIDFVVNRPFSVFPTEVLSVSSLRTPSRLFALLCDFFLYCSKPGPCVEIASFSTPPPCLVSNCVAQIPTHAEMESIRFPTKTLPAGRFLQFHKRKYTKRPETLIIHESGLALKTSALGVTSKPNSRPITVKSASGEKYEAYEISRKDFERSRRRQQTPRVRSHKPRKINKAVEPFFFPEEPKKDKRKRASLPTEDEGFITFGTLRFPLSETPKEEPRLPKFREVEIPVVKKHAVPAVVSKPVRTFRPVATTGAEYVNARNQCSRRPRNHPILRSASYTFGFKKMPLQRFMKEKKEYYVKRSKVVSSCSVTKSPLEALASILKNLPQYSYNSERLKFYDHFIGDDFEIEVHPLRGGKLSVLLILPKGEAYCVVTAATPQYHAALTIARGDRPRVGELLQYRPGEGLCYLAHAALCCALQKRTFREEDFFVGMYPTKFVFAKRLTEKLGPSALKHPVRGRQVSRSLFHCDVASAFSSPFYSLPRFIGGVEEEAPEITSSLKHKAIESVYERVSIHKDNLLARSVEKDLIDFKDEIKSLSKEKRSVTVPFYMGEAVQSGLTRAYPQFNLSFTHSVYSDHPAAAGSRLLENETLASMAKSSFSDIGGCPLFHIKRGSTDYHVCRPIYDMKDAQRRVSRELQARGLVENLSREQLVEAQARVSVCPHTLGNCNVKSDVLIMVQVYDASLNEIASAMVLKESKVAYLTMVTPGELLDEREAFAIDALGCDVVVDTRRDMVQYKFGSSCYCHKLSNIKSIMLTPAFTFSGNLFSVEMYENRMGVNYYKITRSAYSPEIRGVKTLRYRRACTEVVQVKLPRFDKTLKTFLSGYDYIYLDAKFVSRVFDYVVSNCSVVNSKTFEWVWSYIKSSKSRVVISGKVIHRDVHIDLKHSECFAAVMLAVGVRSRTTTEFLAKNLNYYTGDASCFETIRFLFREWSRRAYAEINRSFRKLMKSILSAGLDYEFLDLDNSLQHLLEYSEVEVRVSIAQNGEVDCNEENRVLTEIIAEAADRKSIAQGLSGALSSVPTQPRGGLRGGSRRSGVSFLYNLVEEVGNLFFSVGDAVRFLVKVFKTFSDSPIFRVVRMFLDLAEAASPFVSVVSLCAWLREAVSAFSSWVADRTVSESVKTFVNRTVKRFLNFMSAKTLTKKFFRFFLSASALAKTVVRKAKVILEAYWEVWFESILSDSGEYSAVEFCSSVVITLLTNSGRLLPGFSPSAIITEVLLDLATKISIEVLLKQISPADSTASSALYRRVLSEILSNFRTMGEHGIFTKVFLLCGFLPVFVRKCVALCVPGDMATYARFLEYGVDDLFFLGRSVNSIKNYLCVVAAGLVDSIVDSVVLKLSGVAKERVLGFKSKIIKNFLNVFRKAKVVTRTSSSTDLSEDEYFSCDESKPGLRGGSSRFTLSRLLDIFFNFLKSSKLVIENACFSAYERIERNMKLYFFPLNSSEEEARRLIRCAGDFDYLSDSAFDEDEMLRQAFEQYYSSDDESVTYDGKPTVLRSYLNVSRRFLETFCNGPKFFVKVSNYFKALYSRLLRVLPWVDRNLSDSPGLKGGNEKALLAKFFKTCVITACECVSQICCLRLIRLCWGTPACGLVRLFYITYSSTRVLSRVVVAVAVCPLLVRNELDGLSDGLTNMGVSVFRRLFVALRRALSAYSNSALRRKIIEFIFGNIHHPFDVAVIETNEVAPEPLSPEVDIDVDCDFGSDSESVSSDEVASNPRPGLHGGSRRSSNFLTSLVKVVFKLARRIPRLLFRLRNFVAYFVERRLASKRLKTFIGLARLFDNFSLTSVVYLLQEYDSVLNAFIDVELILLNSGSVNVLPLVSWVRGSLTKLAEAIVGSGFASFLGRMCCRVSDWCSSSSNAGCNFMSPVRTKGKFVPPSSSGSTASMYERLEALESDIREHVLSTCRVGSDEEEERPKEVTEPGIEHTSEDVVPIRSHSQPLSGGECSYSEDREENERANLLPHVSKIVSERRGLETARRNKRTLHGVSEFLNAINTSNEQPRPIIVDHSPESRALTNSVREFYYLQELALFELSCKLREYYDQLKVANFNRQECLCDKDEDMFVLRAGQGVVSGRNSRLPLKHFKGHEFCFRSGGLVPYDGTSRVDTIFHTQTNFVSANALLSGYLSYRTFTFTNLSANVLLYEAPPGGGKTTTLIKVFCETFSKVNSLILTANKSSREEILAKVNRIVLDEGDTPLQTRDRILTIDSYLMNNRGLTCKVLYLDECFMVHAGAAVACIEFTKCDSAILFGDSRQIRYGRCSELDTAVLSDLNRFVDDESRVYGEVSYRCPWDVCAWLSTFYPKTVATTNLVSAGQSSMQVREIESVDDVEYSSEFVYLTMLQSEKKDLLKSFGKRSRSSVEKPTVLTVHEAQGETYRKVNLVRTKFQEDDPFRSENHITVALSRHVESLTYSVLSSKRDDAIAQAIVKAKQLVDAYRVYPTSFGGSTLDVSVNPSTSDRSKCKASSAPYEVINSFLESVVPGTTSVDFGDVSEEMGTQVFESGADNVVIRDSAPVNKSTDHDPQRVSSIRSQAIPKRKPSLQENLYSYESRNYNFTVCERFSGPQEFGQAMAMVMLERSFDLEKVAKVRSDVIAITEKGVRTWMSKREPSQLRALSSDLQKPLNLEEEITTFKLMVKRDAKVKLDSSCLVKHPPAQNIMFHRKAVNAIFSPCFDEFKNRVITCTNSNIVFFTEMTNSTLASIAKEMLGSEHVYNVGEIDFSKFDKSQDAFIKSFERTLYSAFGFDEDLLDVWMQGEYTSNATTLDGQLSFSVDNQRKSGASNTWIGNSIETLGILSMFYYTNRFKALFVSGDDSLIFSESPIRNSADAMCTELGFETKFLTPSVPYFCSKFFVMTGHDVFFVPDPYKLLVKLGASKDEVDDEFLFEVFTSFRDLTKDLVDERVIELLTHLVHSKYGYESGDTYAALCAIHCIRSNFSSFKKLYPKVKGWVVHYGKLKFVLRKFANCFREKFDTAFGERTFLLTTKLETVL |
| Enzyme Length | 3094 |
| Uniprot Accession Number | Q08534 |
| Absorption | |
| Active Site | ACT_SITE 509; /note=For leader protease activity; /evidence=ECO:0000269|PubMed:8259666; ACT_SITE 569; /note=For leader protease activity; /evidence=ECO:0000269|PubMed:8259666 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; |
| DNA Binding | |
| EC Number | 3.4.22.-; 2.1.1.-; 2.7.7.48; 3.6.4.13 |
| Enzyme Function | FUNCTION: L-pro is involved in systemic transport and in RNA amplification. {ECO:0000269|PubMed:12584307}.; FUNCTION: RNA-dependent RNA polymerase replicates the viral genome. {ECO:0000305}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Alternative sequence (1); Chain (3); Coiled coil (1); Domain (4); Region (1); Site (1) |
| Keywords | ATP-binding;Coiled coil;Direct protein sequencing;Helicase;Host cytoplasmic vesicle;Host membrane;Hydrolase;Membrane;Methyltransferase;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Ribosomal frameshifting;Thiol protease;Transferase;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Leader protease]: Host cytoplasmic vesicle membrane. Note=Associates with the closterovirus-induced membranous vesicle aggregates.; SUBCELLULAR LOCATION: [Methyltransferase/helicase/RNA-directed RNA polymerase]: Host cytoplasmic vesicle membrane. Note=Associates with the closterovirus-induced membranous vesicle aggregates. |
| Modified Residue | |
| Post Translational Modification | PTM: The leader protease is released by autoproteolysis. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 348,277 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248; RHEA:13065 |
| Cross Reference Brenda |