| IED ID | IndEnz0002001207 |
| Enzyme Type ID | protease001207 |
| Protein Name |
Replicase polyprotein 1ab pp1ab ORF1ab polyprotein Cleaved into: Non-structural protein 1-non-structural protein 3 fusion nsp1-nsp3 fusion Fragment |
| Gene Name | rep 1a-1b |
| Organism | Murine coronavirus (strain defective JHM) (MHV) (Murine hepatitis virus) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Nidovirales Cornidovirineae Coronaviridae Orthocoronavirinae Betacoronavirus Embecovirus Murine coronavirus Murine hepatitis virus Murine coronavirus (strain defective JHM) (MHV) (Murine hepatitis virus) |
| Enzyme Sequence | MAKMGKYGLGFKWAPEFPWMLPNASEKLGNPERSEEDGFCPSAAQEPKVKGKTLVNHVRVDCSRLPALECCVQSAIIRDIFVDEDPQKVEASTMMALQFGSAVLVKPSKRLSVQAWAKLGVLPKTPAMGLFKRFCLCNTRECVCDAHVAFQLFTVQPDGVCLGNGRFIGWFVPVTAIPEYAKQWLQPWSILLRKGGNKGSVTSGHSRRAVTMPVYDFNATDVVYADENQDDDADDPVVLVADTQEEDGVAKEQVDSADSEICVAHTVGQEMTEPDAVGSQTPIASAEETEVGEACDREGIAEVKATVCADALDACPDQVEAFDIEKVEDSILSELQTELNAPADKTYEDVLAFDAIYSETLSAFYAVPSDETHFKVCGFYSPAIERTNCWLRSTLIVMQSLPLEFKDLGMQKLWLSYKAGYDQCFVDKLVKSAPKSIILPQGGYVADFAYFFLSQCSFKVHANWRCLKCGMELKLQGLDAVFFYGDVVSHMCKCSFKAYF |
| Enzyme Length | 500 |
| Uniprot Accession Number | P26627 |
| Absorption | |
| Active Site | ACT_SITE 389; /note=For PL1-PRO activity; /evidence=ECO:0000255|PROSITE-ProRule:PRU00444 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.; FUNCTION: The papain-like proteinase 1 (PL1-PRO) is responsible for the cleavages located at the N-terminus of the replicase polyprotein. Activity of PL1-PRO is strongly dependent on zinc (By similarity). {ECO:0000250}.; FUNCTION: Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (2); Non-terminal residue (1); Zinc finger (1) |
| Keywords | Decay of host mRNAs by virus;Eukaryotic host gene expression shutoff by virus;Eukaryotic host translation shutoff by virus;Host gene expression shutoff by virus;Host mRNA suppression by virus;Host-virus interaction;Hydrolase;Metal-binding;Methyltransferase;Protease;RNA-binding;Thiol protease;Transferase;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 55,246 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |