IED ID | IndEnz0002001217 |
Enzyme Type ID | protease001217 |
Protein Name |
Replicase polyprotein 1ab Cleaved into: Leader protease L-Pro EC 3.4.22.- Papain-like cysteine proteinase PCP ; Methyltransferase/helicase/RNA-directed RNA polymerase EC 2.1.1.- EC 2.7.7.48 EC 3.6.4.13 |
Gene Name | 1a-1b |
Organism | Grapevine leafroll-associated virus 3 (isolate United States/NY1) (GLRaV-3) (Grapevine leafroll-associated closterovirus (isolate 109)) |
Taxonomic Lineage | Viruses Riboviria Orthornavirae Kitrinoviricota Alsuviricetes Martellivirales Closteroviridae Ampelovirus Grapevine leafroll-associated virus 3 Grapevine leafroll-associated virus 3 (isolate United States/NY1) (GLRaV-3) (Grapevine leafroll-associated closterovirus (isolate 109)) |
Enzyme Sequence | MDYIRPLRVFSFPHVNNTLEYVRYNKANGDVGAFLTTMKFIGNVKLSDFTPRCAAMIYIGKLTKGVKRTFVPPPVKGFARQYAVVSGSVSALRGDGKKVLMEARTSTSATSDVSDFDVVFEAVSNALLVVHYHRVVPYAPVKREQPKPAVKQDEQKPKRQASHWAVKPTAVGVHVPLPKKQEALEPAQSVPQQSLEEKAALTFGLFFSKGGGDESDAVILRKGKLFNRALNVPIDVKNTFVWAKIWDEASRRRGYFYVKDRAVKFFPIVRGRATIEDFIVNTAPGCDVALPRIELWSMRERAFVCTTKGWCWFNNERLRGEIYRRRCFSSSFSIGFLMHLGFRSLKVIRFAGTNILHMPSLNEERTFGWKGGDVYLPNVPKTAIVAGDRTRLGGEILASVANALNQEEVYSSVVSSITNRLVLRDQSALLSHLDTKLCDMFSQRDAMIREKPSHRCDVFLKPREREKLRELFPELSIQFSDSVRSSHPFANAMRSCFNGIFSRRCGNVCFFDIGGSFTYHVKAGHVNCHVCNPVLDVKDVKRRINEILFLSTAGGDSYVSSDLLTEAASKSVSYCSRESQNCDSRADAGFMVDVYDISPQQVAEAMDKKGALVFDIALMFPVELLYGNGEVYLEELDTLVKREGDYLAYNVGQCGEMYEHSFSNVSGFFTFSYVRTSSGNVFKLEYEGYRCGYHHLTMCRAQKSPGTEVTYRSLVPSFVGKSLVFIPVVAGSSVSFKTIVLDSDFVDRIYSYALNTIGTFENRTFEYAVGAVRSQKTHVITGSRVVHSKVDISPDDMWGLVVAVMAQAIKDRAKSIRSYNFIKASEGSLAGVFKLFFQTVGDCFSNAVSVYAKAMVHDNFNVLETLMSMPRAFIRKVPGSVVVTICTSGASDRLELRGAFDISKETFGRKLKNSRLRVFSRAIVEDSIKVMKAMKTEDGKPLPITEDSVYAFIMGNVSNVHCTRAGLLGGSKATVVSSVSKGLVARGAATKAFSGITSFFSTGSLFYDRGLTEDERLDALVRTENAINSPVGILETSRVAVSKVVAGTKEFWSEVSLNDFTTFVLRNKVLIGIFVASLGAAPIAWKYRRGIAANARRYAGSSYETLSSLSSQAAGGLRGLTSSTVSGGSLVVRRGFSSAVTVTRATVAKRQVPLALLSFSTSYAISGCSMLGIWAHALPRHLMFFFGLGTLLGARASANTWKFGGFSNNWCAVPEVVWRGKSVSSLLLPITLGVSLIIRGLLNDTIPQLAYVPPVEGRNVYDETLRYYRDFDYDEGAGPSGTQHEAVPGDDNDGSTSSVSSYDVVTNVRDVGISTNGEVTGEEETHSPRSVQYTYVEEEVAPSAAVAERQGDPSGSGTADAMAFVESVKKGVDDVFHQQSSGETAREVEVDGKGLLPESVVGEAPTQERGRAADGNTAQTAVNEGDREPVQSSLVSSPQADIPKVTQSEVHAQKEVKQEVPLATVSGATPIVDEKPAPSVTTRGVKIIDKGKAVAHVAEKKQVQVEQPKQRSLTINEGKAGKQLCMFRTCSCGVQLDVYNEATIATRFSNAFTFVDNLKGRSAVFFSKLGEGYTYNGGSHVSSGWPRALEDILTAIKYPSVFDHCLVQKYKMGGGVPFHADDEECYPSDNPILTVNLVGKANFSTKCRKGGKVMVINVASGDYFLMPCGFQRTHLHSVNSIDEGRISLTFRATRRVFGVGRMLQLAGGVSDEKSPGVPNQQPQSQGATRTITPKSGGKALSEGSGREVKGRSTYSIWCEQDYVRKCEWLRADNPVMALEPDYTPMTFEVVKTGTSEDAVVEYLKYLAIGIERTYRALLMARNIAVTTAEGVLKVPNQVYESLPGFHVYKSGTDLIFHSTQDGLRVRDLPYVLIAEKGIFTKGKDVDAVVALGDNLFVCDDILVFHDAINLIGALKVARCGMVGESFKSFEYKCYNAPPGGGKTTTLVDEFVKSPNSTATITANVGSSEDINMAVKKRDPNLEGLNSATTVNSRVVNFIVRGMYKRVLVDEVHMMHQGLLQLGVFATGASEGLFFGDINQIPFINREKVFRMDCAVFVPKKESVVYTSKSYRCPLDVCYLLSSMTVRGTEKCYPEKVVSGKDKPVVRSLSKRPIGTTDDVAEINADVYLCMTQLEKSDMKRSLKGKGKETPVMTVHEAQGKTFSDVVLFRTKKADDSLFTKQPHILVGLSRHTRSLVYAALSSKLDDKVGTYISDASPQSVSDALLTRSPRLVAFEVYERMNFGPTFEGELVRKIPTSHFVAVNGFLEDLLDGCPAFDYDFFEDDFETSDQSFLIEDVRISESFSHFTSKIEDRFYSFIRSSVGLPKRNTLKCNLVTFENRNFNADRGCNVGCDDSVAHELKEIFFEEVVNKARLAEVTESHLSSNTMLLSDWLDKRAPNAYKSLKRALGSFVFHPSMLTSYTLMVKADVKPKLDNTPLSKYVTGQNIVYHDRCVTALFSCIFTACVERLKYVVDERWLFYHGMDTAELAAALRNNLGDIRQYYTYELDISKYDKSQSALMKQVEELILLTLGVDREVLSTFFCGEYDSVVRTMTKELVLSVGSQRRSGGANTWLGNSLVLCTLLSVVLRGLDYSYIVVSGDDSLIFSRQPLDIDTSVLSDNFGFDVKIFNQAAPYFCSKFLVQVEDSLFFVPDPLKLFVKFGASKTSDIDLLHEIFQSFVDLSKGFNREDVIQELAKLVTRKYKHSGWTYSALCVLHVLSANFSQFCRLYYHNSVNLDVRPIQRTESLSLLALKARILRWKASRFAFSIKRG |
Enzyme Length | 2772 |
Uniprot Accession Number | O71189 |
Absorption | |
Active Site | ACT_SITE 311; /note=For leader protease activity; /evidence=ECO:0000250; ACT_SITE 357; /note=For leader protease activity; /evidence=ECO:0000250 |
Activity Regulation | |
Binding Site | BINDING 1685; /note=2-oxoglutarate; /evidence=ECO:0000255|PROSITE-ProRule:PRU00805 |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; |
DNA Binding | |
EC Number | 3.4.22.-; 2.1.1.-; 2.7.7.48; 3.6.4.13 |
Enzyme Function | FUNCTION: RNA-dependent RNA polymerase replicates the viral genome. {ECO:0000305}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Binding site (1); Chain (3); Compositional bias (3); Domain (5); Erroneous gene model prediction (1); Metal binding (3); Region (4); Site (1) |
Keywords | ATP-binding;Dioxygenase;Helicase;Hydrolase;Iron;Metal-binding;Methyltransferase;Nucleotide-binding;Nucleotidyltransferase;Oxidoreductase;Protease;RNA-directed RNA polymerase;Reference proteome;Ribosomal frameshifting;Thiol protease;Transferase;Viral RNA replication |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 306,516 |
Kinetics | |
Metal Binding | METAL 1619; /note=Iron; /evidence=ECO:0000255|PROSITE-ProRule:PRU00805; METAL 1621; /note=Iron; /evidence=ECO:0000255|PROSITE-ProRule:PRU00805; METAL 1676; /note=Iron; /evidence=ECO:0000255|PROSITE-ProRule:PRU00805 |
Rhea ID | RHEA:21248; RHEA:13065 |
Cross Reference Brenda |