| IED ID | IndEnz0002001225 |
| Enzyme Type ID | protease001225 |
| Protein Name |
RNA replication polyprotein ORF1 protein Cleaved into: Helicase EC 3.6.4.13 Includes: Viral methyltransferase EC 2.1.1.- ; Putative Fe 2+ 2-oxoglutarate dioxygenase EC 1.14.11.- ; Protease EC 3.4.22.- ; RNA-directed RNA polymerase EC 2.7.7.48 |
| Gene Name | ORF1 |
| Organism | Apple stem pitting virus (isolate PA66) (ASPV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Kitrinoviricota Alsuviricetes Tymovirales Betaflexiviridae Quinvirinae Foveavirus Apple stem pitting virus Apple stem pitting virus (isolate PA66) (ASPV) |
| Enzyme Sequence | MALLSRTAAEEVIASFTSEEQSRISTQAVLALTNVEKDKHDLFNYALPELAKMRLFNSGIYLSPHSYRPHSHPVCKTLENNILFNILPSYLDNSFYLVSIKKNKVDFLKRRHPDLQMVETINRYISSIDKTRYGGFFHVSPSKISAKFKCDRRTGFEDDASLIDLIPGCMEGARKRFFFHDELHYWTKEALITFLDHVKPEVMLASIVFPPEILAGAKESLNPWCYTFRIVGKDLVFFPDGEQSEAYIQPVAGSYLLRTGKITTPSGDIFQLDLLKSSFSHHLISITKGEAIGQKMRFFNGFEAVAMKGLNPLRRKVESCLPISKNTILKIYRYLRTLKKPDLQSAMAKLSQVCKDPNGYEIKFFEEFSKLCLKCDTLNTNMIPDMKRIVQGFFLKLFPNPISRNFKVVQQLHLDNFIETLEEFNFSINTESLSLNWKDDLEFVNLTFGDTDFNVEDSFAEAWGTKKDVVNITTVHHSPYLVSKFESYDHQFHSILSVKSISALTRIAKIVLSLYDPCVVEAFSESRVTNLAVNVIIAANLRACFAVTDLWRIFEGILLKECKRAQGKMRKRFHFELGIRWFLFVDVSNQWFLPPCRDGLIARSVSFDQFIKGCQRDNSLHNGRMSLRQVLKGPKLQALFDVSELSIIHNVEMENAPEAGSTLDAGIKPTSSPLEVVPIENARCNLAPCKCDLNCFIQPADVNSLHGNLVFLDFIGGSKGRGASFYSRDLKGYSYTGFSHVSRGWPAFLDKFLSDNKIPLNFYNQCLVQEYSTGHGLSMHKDDESIYDINHQVLTVNYSGDAIFCIECLGSGFEIPLSGPQMLLMPFGFQKEHRHGIKSPSKGRISLTFRLTKEGDSQVPIQEVVTICDHGDSDDRAALKALERRSHQSGGRPAVELEGHEREKVNSDSSDSAPVQEFLIQIDSSLLEYALKSLSGLSKNVVNCDMCLCNSPWLKNEELRFSEALRDLAFAQGLIQLIDFLCLKVLRCAEVNRIISELPTHVFPLRGTMHIVDLDDESIRGDVKEGSFSGFRRWKVMSCSTDLIMLAFLKPKMTLGGELRSHEDECELSDLTEKLHGCSVILSRKFEPDLFHSFDVEADGNCFWHSVGPLIGVDGEYLKRILHDQAKKDGVKCPRLSKQLEGNTWAEREAVAYFCSHYGIRLNVLYTREECTWIFKPHEVLKAATLICQDNHFKPCMPVNGCVIRAISSALNRREVDVLAVLGKPAHEDLFEEVAEGRGFSIFDLTRLFEIFSICGSVDTGGELIMVNENGRIPAEFSLEKEHLAHIPTLSRRKFSPIVSDLNRVSNSAMRFLAINGAEVDYRPSIDRASTLLDSFEIGATGVLCQGIKEAQKDLASKLIPELVHERKLIMILGTFGCGKSSLFKKFIEKSPGKAITFVSPRRSLAESINHDLGLARVGGKKTGKSKDLKNVRVKTFELFILHLDSIKEGHTVVIDEIQLFPPGYIDLIILGLKPNVNIIIAGDPCQSDYDCSSDRHIFAGSESDIMRILSGRSYKFNILSQRFRNPVFYGRLPCNLNKTRLTLDEEEYTLWDSIQEFSMMGRKDCPVVLVSSFEEKKIVAAHLGLKMKCITYGESTGLNFQKGAILVTYESALTSDRRWWTALSRFSHDIHFINGMGVTWDNAITHFVGKPLHKFFTKRACNDDIIDLLPGRPELIEGFQSQVGADEGVREAKLVGDPWLKTKIFLGQNPDFEIEIADEVEAAEDWFKTHIPIMSLEAVRAQWVHKLISREDREFRIGDITTEQFTDDHSKNRGQELTNAAERYEAIYPRHKGTDTATFLMAVKKRLSFSSPAAEHAKLRRAKPFGKFLLDTFLKRVPLNSSHDEKMMQEAVHAFEEKKLSKSMATIENHSGRSCEDWPVDKALIFMKSQLCTKFDNRFRSAKAGQTLACFQHSVLCRFAPYMRYIESKVTEVLPKNLYIHSGKNIDDLAAWVTTSKFNGVCTESDYEAFDASQDHFILAFELEVMKFLGLPSDLIADYTFIKTHLGSKLGSFAIMRFTGEASTFLFNTMANMLFTFLRYDLNGREAICFAGDDMCANSRLKVTNRFSNFLDKIKLKAKVQFTATPTFCGWGLCEHGVFKKPDLVLERLQIARETRNLENCIDNYAIEVSCAYKMGENLNLYLTPQEVDAHYNCVRFIVQHNHLLKSNIRDLFKGESLPASS |
| Enzyme Length | 2183 |
| Uniprot Accession Number | Q64962 |
| Absorption | |
| Active Site | ACT_SITE 1202; /evidence=ECO:0000250|UniProtKB:Q65652; ACT_SITE 1283; /evidence=ECO:0000250|UniProtKB:Q65652 |
| Activity Regulation | |
| Binding Site | BINDING 844; /note=2-oxoglutarate; /evidence=ECO:0000255|PROSITE-ProRule:PRU00805 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; Evidence={ECO:0000305}; CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 3.6.4.13; 2.1.1.-; 1.14.11.-; 3.4.22.-; 2.7.7.48 |
| Enzyme Function | FUNCTION: [RNA replication polyprotein]: RNA-directed RNA polymerase involved in viral RNA replication. {ECO:0000250|UniProtKB:Q65652}.; FUNCTION: Protease: Thiol protease that cleaves the polyprotein. {ECO:0000250|UniProtKB:Q65652}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 1374..1381; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00990 |
| Features | Active site (2); Binding site (1); Chain (2); Compositional bias (1); Domain (7); Metal binding (3); Nucleotide binding (1); Region (1); Site (1) |
| Keywords | ATP-binding;Dioxygenase;Helicase;Hydrolase;Iron;Metal-binding;Methyltransferase;Multifunctional enzyme;Nucleotide-binding;Nucleotidyltransferase;Oxidoreductase;Protease;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages by the viral protease yield mature proteins. {ECO:0000250|UniProtKB:Q65652}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 247,262 |
| Kinetics | |
| Metal Binding | METAL 780; /note=Iron; /evidence=ECO:0000255|PROSITE-ProRule:PRU00805; METAL 782; /note=Iron; /evidence=ECO:0000255|PROSITE-ProRule:PRU00805; METAL 835; /note=Iron; /evidence=ECO:0000255|PROSITE-ProRule:PRU00805 |
| Rhea ID | RHEA:13065; RHEA:21248 |
| Cross Reference Brenda |