| IED ID | IndEnz0002001228 |
| Enzyme Type ID | protease001228 |
| Protein Name |
Replicase 237 kDa protein Cleaved into: Replicase large subunit EC 2.1.1.- EC 2.7.7.- EC 3.4.22.- EC 3.6.4.13 150 kDa protein ; Replicase small subunit EC 2.7.7.48 66 kDa protein RdRp |
| Gene Name | ORF1 |
| Organism | Beet necrotic yellow vein virus (isolate Japan/S) (BNYVV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Kitrinoviricota Alsuviricetes Hepelivirales Benyviridae Benyvirus Beet necrotic yellow vein virus Beet necrotic yellow vein virus (isolate Japan/S) (BNYVV) |
| Enzyme Sequence | MADSFGFTPMEVLLFGGESVQLLTSDMPIDVQWGFVHSTRCYALWKDDLIHLNPLLKYSQRIAKRWERLVSGFVGPVPLDKLLSLLAKLMRYCVNMGVSVQEIYLSDAIVSSSYMLHVSRSAGCVSFSWLYAKLSMFASCGKFWVGSSHHTAANMIEGSRAVNGPDVAISEMVEAFHLEVKSSLVVTVSLTPREKKILERELGFVPLYKQKSRAPRNHPVLAALREVMRQEYSASCNILNTKLKTLVVGAASREVNCYSSNPSVHYYFANKDSKDLVRTTLELLHSALATKYRNMESGERELMNNLKGCGYIVKRSVENAVYEVVSDKDVAEVLRYAQTVASTKKEAKKKPNTGKRKMVMSEATRRTIELHELSRIVAEEKKIPNHFHFDESDFASVGNFTQLVCEDVGYNFSVDAWLHLFEATGAQTAVGYMALPNELLFEHYPISDYYDYWEGVEKHGSLGGITISPLRNGQVVGMPTGVFQPVHFDKTSAGLGIPGSKMGAAERVICHMSDGLGNGYNHVKSDWQTLLKHPILSSSKYNFAVEVDLTGRYGCLATFRLTRVTGVKYVARTIKLRPEDRYVRVLDLLHIVRSIRLKGHAGLKEPYQYFPVYKREVDTPVSYCFSIAEKSLTVQNIANFIRHHIGGVSLVNKELVSAWRLNPQLVPSFAYAVYFYVVNLRGELDGMLQKLMKKGITWADRLKANVSAFLRDMVDPISFLWTWLFERRLVDQIFQDGTDVFYQMDRACVDEKALRLNDHIKITRDFLPADTLLPEGWSLDDWEKAPDSLKTLSAAASLPVECGAVNCVGKSFKSVRTLLPPSVVTSPVEQFFKSGGKFRDDAEFAELLSAHYRWQMDNSFCACQVCAALTGKTGSQVVECRWKAESMYTFSMSQTEVDDFRNEIKAQSIEKGNRFGEMLIGVHQKIPTQAFEVSVRLEYVKGGPGTGKSFLIRSLADPIRDLVVAPFIKLRSDYQNQRVGDELLSWDFHTPHKALDVTGKQIIFVDEFTAYDWRLLAVLAYRNHAHTIYLVGDEQQTGIQEGRGEGISILNKVDLSKVSTHVPIMNFRNPVRDVKVLNYLFGSRMVPMSSVEKGFSFGDVKEFSSLSNIPDTKIIHYSDETGEHMMPDYVRGVSKTTVRANQGSTYDNVVLPVLPSDLNLINSAELNLVALSRHRNKLTILLDNDGMNIGAVLKGMLEGVPEELERRDYIVGMYLGLHLPIKKEFFFPESEFAKSFRLMVAKYEAFVPYDSNLPTLVLQGDVVVLDIARVENDINDAFNCPDFYNLVSRPNNCLVVAISECLGVTLEKLDNLMQANAVTLDKYHAWLSKKSPSTWQDCRMFADALKVSMYVKVLSDKPYDLTYEVDGAGSSVTLHLVGKESDGHFIAAPLSPSLSTNERESGHDSKKPADDSDTFDAANLFADKGVSSADIEAFCAYLEKTLMATIMEYDLRLQSWANVVDDTDDFYQINISEFRQSTCFGKLLSALEVLKVDVSRKRFISDWLCKNLENKQFRWRWSSSVASTSSAGSNVDDDFVNMAGGKTDANVDPADVLRQSFMDYASEFVPILIAESPILMPLVEPEPILSKCMVPEFDAFLLIKEFDLDNGADEYQCAYLNESVANRVGDKFVSGVLDTDIISPLNLRGHPIAENVKYHSMCVAPAQIYFKRNQWQELQVQQARYLFRKVRNSPSSTQDSVARMVAQLFVSDCLVPNVADTFSASNLWRIMDKAMHDMVTKNYQGQMEEEFTRNAKLYRFQLKDIEKPLKDPETDLAKAGQGILAWSKEAHVKFMVAFRVLNDLLLKSLNSNVVYDNTMSETEFVGKINAAMNIVPDSAINGVIDAAACDSGQGVFTQLIERHIYAALGISDFFLDWYFSFREKYVMQSRYVRAHMSYVKTSGEPGTLLGNTILMGAMLNAMLRGTGPFCMAMKGDDGFKRQANLKINDQMLKLIKKETVLDFKLDLNVPITFCGYALSNGHLFPSVSRKLTKIAAHRFREYKHFCEYQESLRDWIKNLPKDPAVYADFLECNASLSCRNVDDVQRWLDAIISVSRIGREQFMMMFPIREVFMSLPPVEDSLGELSSTKVAVSIGDNVSNVVRKVARVDMKKF |
| Enzyme Length | 2109 |
| Uniprot Accession Number | Q65667 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; |
| DNA Binding | |
| EC Number | 2.1.1.-; 2.7.7.-; 3.4.22.-; 3.6.4.13; 2.7.7.48 |
| Enzyme Function | FUNCTION: [Replicase small subunit]: Is an RNA-dependent RNA polymerase active in viral RNA replication. {ECO:0000305}.; FUNCTION: [Replicase large subunit]: Is a methyltransferase active in RNA capping and an RNA helicase. Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. Helicase region probably exhibits NTPase and RNA unwinding activities. Contains a cysteine protease activity responsible for autocatalytic processing of the 237 kDa protein (Potential). {ECO:0000305}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (3); Domain (3); Region (3) |
| Keywords | ATP-binding;Coiled coil;Helicase;Hydrolase;Methyltransferase;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-binding;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Viral RNA replication |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Autocatalytically cleaved by the cysteine protease. {ECO:0000269|PubMed:9191870}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 237,518 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21248; RHEA:13065 |
| Cross Reference Brenda |