| IED ID | IndEnz0002001231 |
| Enzyme Type ID | protease001231 |
| Protein Name |
Zinc metalloprotease RasP EC 3.4.24.- Regulating alternative sigma factor protease Regulating anti-sigma-W factor activity protease |
| Gene Name | rasP ABC2235 |
| Organism | Bacillus clausii (strain KSM-K16) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Alkalihalobacillus Alkalihalobacillus clausii (Bacillus clausii) Bacillus clausii (strain KSM-K16) |
| Enzyme Sequence | MNTLLAFIAIFSVLVFVHEWGHLYFAKKAGILCYEFAIGMGPKLFAFERNDTIYTIRLLPIGGYVRMAGEEPEQPTIRPGYEIGLVLDEKDTVKELIVNNKSKHPEAQVVQVERIDLVHDLFVETIDEDTGELVRYPIDEKAFIVQDEVAQIIAPWKRQFGSKPLPKRAMAIFAGPLMNFILGFVILLGLSLYQGVTLSSEIVINGENSPAEAAGLQDGDVITAVNGVEVDSWKEMTTEVKKYPGEEVSIDYERNGEALQTNATLSQVEVMPDEYEGFLGVSGVPEFSLLGSLQYAGNEFINMATSIFDTLGLIFTGQFSLDYISGPVGIYDITDQAVSLGIQTVIFFAALLSINLGVINLMPIPALDGGRLMFLAYEGIRGKPVSPEKEGAIQFIGFALVMLLMIVVTWNDISKLFS |
| Enzyme Length | 418 |
| Uniprot Accession Number | Q5WFT5 |
| Absorption | |
| Active Site | ACT_SITE 19; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Is responsible for Site-2 cleavage of the RsiW anti-sigma factor. This results, after a third proteolytic step catalyzed by the ClpXP protease, in the release of SigW and the transcription activation of the genes under the control of the sigma-W factor (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Domain (1); Metal binding (2); Transmembrane (4) |
| Keywords | Cell membrane;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Transmembrane;Transmembrane helix;Zinc |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 46,057 |
| Kinetics | |
| Metal Binding | METAL 18; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 22; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda |