Detail Information for IndEnz0002001250
IED ID IndEnz0002001250
Enzyme Type ID protease001250
Protein Name RHOMBOID-like protein 12, mitochondrial
AtRBL12
EC 3.4.21.-
Presenilins-associated rhomboid-like protein
Gene Name RBL12 PARL At1g18600 F25I16.6
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MKAIFNRRVVVDSSSRLTKLLANPTTHSHLNRQTFTSLYKPNQSRHFRTHYLPSSPSSPPVSRFDPSQLWRSEKIRGFFASALGNKAVKLGNLVESRVGFIGSQFPKKGFEFQRFSGFQRRGWKHWLQGLSDRDVVLGLVIANAGVFVMWRVFNQQFMMNNFMISLDNFKSGRLHTLITSAFSHIDIGHIVSNMIGLYFFGTSIARNFGPQFLLKLYLAGALGGSVFYLIHHAYMAATSPKGQGAFVRDPSRTPGLGASGAVNAIMLLDIFLHPRATLYLEFFIPVPAMLLGIFLIGKDILRITEGNSNISGSAHLGGAAVAAIAWARIRKGRFRF
Enzyme Length 336
Uniprot Accession Number Q9FZ81
Absorption
Active Site ACT_SITE 259; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P54493; ACT_SITE 315; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P54493
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.21.-
Enzyme Function FUNCTION: Probable rhomboid-type serine protease that catalyzes intramembrane proteolysis. Unable to cleave either of the yeast Pcp1 substrates in yeast cells. {ECO:0000269|PubMed:18543065}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Transit peptide (1); Transmembrane (6)
Keywords Hydrolase;Membrane;Mitochondrion;Protease;Reference proteome;Transit peptide;Transmembrane;Transmembrane helix
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000269|PubMed:18543065, ECO:0000269|PubMed:22738221}; Multi-pass membrane protein {ECO:0000255}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 18650403;
Motif
Gene Encoded By
Mass 37,441
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda