| IED ID | IndEnz0002001262 |
| Enzyme Type ID | protease001262 |
| Protein Name |
Subtilisin-like protease SBT1.7 EC 3.4.21.- Cucumisin-like serine protease Subtilase subfamily 1 member 7 AtSBT1.7 Subtilisin-like serine protease 1 At-SLP1 |
| Gene Name | SBT1.7 ARA12 ASP48 SLP1 At5g67360 K8K14.8 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MSSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT |
| Enzyme Length | 757 |
| Uniprot Accession Number | O65351 |
| Absorption | |
| Active Site | ACT_SITE 139; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 212; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 542; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | ACTIVITY REGULATION: Activated by calcium. Inhibited by the serine protease inhibitors 4-(2-aminoethyl)benzenesulphonyl fluoride (AEBSF), PMSF, di-isopropyl phosphofluoridate (DFP) and soybean trypsin inhibitor (SBTI). Not inhibited by benzamidine or iodoacetamide. Leupeptin and pepstatin A have a minor inhibitory action. {ECO:0000269|PubMed:12413398}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease. Has a substrate preference for the hydrophobic residues Phe and Ala and the basic residue Asp in the P1 position, and for Asp, Leu or Ala in the P1' position (PubMed:12413398). Essential for mucilage release from seed coats. Triggers the accumulation and/or activation of cell wall modifying enzymes necessary either for the loosening of the outer primary cell wall, or to facilitate swelling of the mucilage (PubMed:18266922). {ECO:0000269|PubMed:12413398, ECO:0000269|PubMed:18266922}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 80 degrees Celsius. Thermostable. {ECO:0000269|PubMed:12413398}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.0. {ECO:0000269|PubMed:12413398}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Domain (2); Glycosylation (6); Propeptide (1); Region (1); Sequence conflict (5); Signal peptide (1) |
| Keywords | Calcium;Cell wall;Direct protein sequencing;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | INDUCTION: By methyl jasmonate. {ECO:0000269|PubMed:12702015}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:12413398, ECO:0000269|PubMed:9188482}. Note=Intracellular spaces and cell wall. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12185496; 14697270; 15593128; 15694452; 15763661; 15769804; 16553893; 16679420; 16895613; 17028149; 17187690; 17227549; 17526915; 18538804; 18650403; 19136719; 20118269; 20639575; 23362209; 23738689; 25043023; 25531128; 25658798; 28003327; 28627464; 32907884; |
| Motif | |
| Gene Encoded By | |
| Mass | 79,415 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |