| IED ID | IndEnz0002001263 |
| Enzyme Type ID | protease001263 |
| Protein Name |
Subtilisin-like protease SBT2.4 EC 3.4.21.- Protein ABNORMAL LEAF SHAPE 1 Subtilase subfamily 2 member 4 AtSBT2.4 |
| Gene Name | SBT2.4 ALE1 At1g62340 F2401.7 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | METNPRKLRSYSYICLIVCIFVLVVCAILSRAEEKEGKGENDDHIPKIYSILVEGEPLAFRASTNINSKAMALEAKKIEEIHDEILGSTLEKGSYTKLYSFKHVINAIAVRTTASQAKKLGKTKGVKAVEEDKGVKLMTTYTPDFLELPQQVWQKISNEGDRRAGEDIVIGFVDTGINPTHPSFAALDLTNPYSSNLSRLHFSGDCEIGPFFPPGSCNGKIISARFFSAGARASGALNSSLDILSPFDASGHGSHVASIAAGNAGVPVIVDGFFYGRASGMAPRSRIAVYKAIYPSIGTLVDVIAAIDQAIMDGVDVLTLSVGPDEPPVDKPTVLGIFDLAMLLARKAGVFVVQAVGNNGPSPSSVLSYSPWVVGVAAGNTDRSYPAPLILDGGQTVQGVGLSGPTLGAPLVQHRLVLAKDAVRTNGSVLQPLTRDIEECQRPENFDPAAVFGSIVICTFSDGFYNQMSTVLAITQTARTLGFMGFILIANPRFGDYVAEPVIFSAPGILIPTVSAAQIILRYYEEKTFRDTRGVATQFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATRSPLDVLKPDILAPGHQIWGAWSLPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLNPSWTPAMIASAISTTANEYDSNGEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLVLPAGFEDYISFLCSLPNISPATIRDATGVLCTTTLSHPANLNHPSVTISALKESLVVRRSFQDVSNKTETYLGSVLPPNGTTVRLTPTWFTVPPQKTQDLDIEFNVTQVLNKFTFGEVVLTGSLNHIIRIPLSVKTI |
| Enzyme Length | 832 |
| Uniprot Accession Number | F4HYR6 |
| Absorption | |
| Active Site | ACT_SITE 174; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 252; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 618; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Serine protease required for epidermal surface formation in embryos and juvenile plants (PubMed:11731449, PubMed:17376810, PubMed:23318634). Involved in embryonic cuticle formation downstream of BHLH95/ZOU (PubMed:23318634). {ECO:0000269|PubMed:11731449, ECO:0000269|PubMed:17376810, ECO:0000269|PubMed:23318634}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (3); Erroneous gene model prediction (1); Glycosylation (6); Mutagenesis (1); Sequence conflict (18); Signal peptide (1) |
| Keywords | Developmental protein;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20736450; 23531691; 24147836; 30998684; 31334862; |
| Motif | |
| Gene Encoded By | |
| Mass | 89,066 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |