| IED ID | IndEnz0002001265 |
| Enzyme Type ID | protease001265 |
| Protein Name |
RNA polymerase sigma factor RpoS Sigma S Sigma-38 |
| Gene Name | rpoS appR katF nur otsX sigS b2741 JW5437 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEQEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE |
| Enzyme Length | 330 |
| Uniprot Accession Number | P13445 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | DNA_BIND 288..307; /note=H-T-H motif; /evidence=ECO:0000255|HAMAP-Rule:MF_00959 |
| EC Number | |
| Enzyme Function | FUNCTION: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response. Controls, positively or negatively, the expression of several hundred genes, which are mainly involved in metabolism, transport, regulation and stress management. {ECO:0000255|HAMAP-Rule:MF_00959, ECO:0000269|PubMed:15558318, ECO:0000269|PubMed:15716429, ECO:0000269|PubMed:16511888, ECO:0000269|PubMed:21398637, ECO:0000269|PubMed:8475100}.; FUNCTION: Protects stationary phase cells from killing induced by endoribonuclease MazF. {ECO:0000269|PubMed:19251848}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); DNA binding (1); Erroneous initiation (2); Erroneous termination (1); Helix (6); Motif (1); Mutagenesis (4); Region (4); Sequence conflict (6); Turn (2) |
| Keywords | 3D-structure;Cytoplasm;DNA-binding;Reference proteome;Sigma factor;Stress response;Transcription;Transcription regulation |
| Interact With | P0A8V2 |
| Induction | INDUCTION: Subject to complex regulation at multiple levels (transcription, translation, regulation of activity and degradation). Transcription is induced during entry into stationary phase and in response to different stresses. Transcription is repressed by antitoxin MqsA (PubMed:21516113). mRNA stability is regulated by small RNA regulators and various proteins such as Hfq, CsdA, CspC and CspE. Finally, the cellular level of RpoS is regulated by proteolysis via RssB and the ClpXP protease. {ECO:0000269|PubMed:10339606, ECO:0000269|PubMed:10672187, ECO:0000269|PubMed:21516113, ECO:0000269|PubMed:21639793, ECO:0000269|PubMed:8475100, ECO:0000269|PubMed:8637901}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00959}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (1); Electron microscopy (1); X-ray crystallography (20) |
| Cross Reference PDB | 5IPL; 5IPM; 5IPN; 6FI7; 6IDO; 6KJ6; 6UTV; 6UTW; 6UTX; 6UTY; 6UTZ; 6UU0; 6UU1; 6UU2; 6UU3; 6UU4; 6UU5; 6UU6; 6UU7; 6UUA; 6UUB; 6UUC; |
| Mapped Pubmed ID | 15690043; 24049175; 24561554; 27035955; 29789383; 31293000; 31846423; 32829286; |
| Motif | MOTIF 118..121; /note=Interaction with polymerase core subunit RpoC |
| Gene Encoded By | |
| Mass | 37,972 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |