| IED ID | IndEnz0002001276 |
| Enzyme Type ID | protease001276 |
| Protein Name |
Serine protease/ABC transporter B family protein tagA EC 3.4.21.- |
| Gene Name | tagA DDB_G0293002 |
| Organism | Dictyostelium discoideum (Slime mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Amoebozoa Evosea Eumycetozoa Dictyostelia (dictyostelid cellular slime molds) Dictyosteliales Dictyosteliaceae Dictyostelium Dictyostelium discoideum (Slime mold) |
| Enzyme Sequence | MNKKLFIFGLSLFLFLFIFNLSLSLKLSSKNNFYNHNHINNQIHRNNEGNNKLSKLIHLHNDVIDTTISNRDNILFNKKSLNQKSKGSLFLVHLNGPIEKQVHNELIKQLDQLFNGGEIIHYIPDNTYLISMIGSDNNDNNNNNKIELINRLKELIPSIQWLKPLEPRLKISPLFKQNQFQGDNQNEIDQLRIYYHENSNQQSNDIDNIISESSLTLVEKELISNNNNNNNNVLITVNLKNSKLSLESIIYKISTRSLVYWIEPSSSKLIKHTPSNKFAHYSIQSGSASTTSTPIWDVIGIKGDGEIVGCADTGIDINHCFFYDTNPIGSTHRKIISYSSGNGDQIDEIDGHGTHIVGTIIGSTTVDPSVSEFSGGAPNSKVAFVDLQVGSGNGLSIQSNLTAIYQSTYDQNAKVHCDAWNSNIGPFYTGVTEMIDRFQWDHPDFLVVRSAGNNVNFGFNSIYTLSQESTSKNSLVVGSSNQPSSTYLSSIDYWDWDFIYNSIRTSVCTQGQSIYGITCSDVPTQTTSVDIQTQCCSNPILAKICCSTEIQQQYQTNSTVYSEFIPSLFSGVGPTSDGRLKPDLLAPGSPIISSRSLGPSSTINHCSPITSGIATSALIAMEGSSQAAAVATSAAVLVRQYYRDGYFINGKVNSSVGFQPSASLVKATLINTASINVDSTLEYSQGFGNIQLSKLITTTNAQTTSLDIPSSIEKADPIINTGETNSYCFSLDSKADIDITLVWTDPAGSPLSTFTLVNNLDLALLAFVDGELSIYSGNSETIFKNTSQVIFDQLNNVEVIRIKDAPIGSYDVKIFGTNIVIPNQSYSVVIRTSGGTTLMKESECAQCFYDPNDDQSQMCEFDNGIGTQYCKDDNRFSKCVVYECNTGYVFDNGITKSCVTTLALTLYDIVLLGIFGIIIVGAVIFVLVCYKSKSLDQNKYFSLSKDKGGDGNSIRSNSVAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNSNGKQSNIELNSVGGGDDGTPNGDDQQQQNNSPQYDEDGRLISGQEVEISIFEVISLGKPESKILGLALFLSFIDVALGLAVPLVAANIFDYLYAGETGKISTTILTFALIIIGMIIVQFLSGILLALAGHRIIARLRREMFASLLKQDMAFFNERKTGELMSRLASDVSSVRSIISDSIPHMIIQIATIGGTLIMLFIISWKLSLVVLCPLPILLVFSKFYGGYIEVISVKVQDALADAATHAAETLFNMKTVRWFSAEEREVAKFSKLISVSYKIALKMTIWNGIYSSTSGIFEQLSVFILLWYGSSLVSNGDLTPSMLIAFNLFLPFITGAVTQVASLYTTYKSYKGSSYRFFEIMQRVPDIQGEGGITRTKVRGDIQFNKVSFAYSSNPDQLVLEKIDIKFEPGTITALIGPSGGGKSTMLSLIGRLYNIDGGSITLDGTNIKDFNVPNLHEHISIVNQEPSLFSGSIAENIMFGKPTATRSEIIEACKQANAHDFITAMPEGYDTLIGERGTALSGGQKQRIAIARTIIKNPTVLLLDETTSELDVESEKLVQDSIDKLVVGRTVIIVAHRLTTILTADIIAVVSDSTISEMGTPEELLAKKGMFYDFVQIQYGKQGEELDIQLPSNSRNTRNADKLRNRSETIKQIAKINNIIPIHRPQRGDDDNEDDENNSGQGSSRSPPPMWRQAKKNANVNKSMLLTRRNTHVQHQSSSGGWQKGNVDDKLQRVLQKSRKKGFMNNQDHKDIKATLVLY |
| Enzyme Length | 1752 |
| Uniprot Accession Number | Q9GTN7 |
| Absorption | |
| Active Site | ACT_SITE 312; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 352; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 625; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Required for a general cell fate determination at the onset of development. Required for the specification of an initial population of prespore cells in which tagA is expressed. Required for normal SDF-2 signaling during spore encapsulation. {ECO:0000269|PubMed:12756178, ECO:0000269|PubMed:17056744}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 1409..1416; /note=ATP; /evidence=ECO:0000255|PROSITE-ProRule:PRU00434 |
| Features | Active site (3); Chain (1); Compositional bias (3); Domain (3); Glycosylation (10); Nucleotide binding (1); Region (2); Signal peptide (1); Transmembrane (7) |
| Keywords | ATP-binding;Differentiation;Glycoprotein;Hydrolase;Membrane;Nucleotide-binding;Protease;Reference proteome;Serine protease;Signal;Transmembrane;Transmembrane helix;Transport |
| Interact With | |
| Induction | INDUCTION: Down-regulated by phagocytic stimuli (when grown on E.coli). {ECO:0000269|PubMed:18559084}. |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000255|PROSITE-ProRule:PRU00441}; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12456012; 15964562; 21585361; |
| Motif | |
| Gene Encoded By | |
| Mass | 192,812 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |