Detail Information for IndEnz0002001287
IED ID IndEnz0002001287
Enzyme Type ID protease001287
Protein Name Penicillin-insensitive murein endopeptidase
EC 3.4.24.-
D-alanyl-D-alanine-endopeptidase
DD-endopeptidase
Gene Name mepA SEN2365
Organism Salmonella enteritidis PT4 (strain P125109)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella enteritidis Salmonella enteritidis PT4 (strain P125109)
Enzyme Sequence MKKTAIALLAWFVSSASLAATPWQKITHPVPGAAQSIGSFANGCIIGADTLPVQSDNYQVMRTDQRRYFGHPDLVMFIQRLSHQAQQRGLGTVLIGDMGMPAGGRFNGGHASHQTGLDVDIFLQLPKTRWSQAQLLRPQALDLVSRDGKHVVPSRWSSDIASLIKLAAQDNDVTRIFVNPAIKQQLCLDAGNDRDWLRKVRPWFQHRAHMHVRLRCPADSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVL
Enzyme Length 274
Uniprot Accession Number B5R3R4
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.24.-
Enzyme Function FUNCTION: Murein endopeptidase that cleaves the D-alanyl-meso-2,6-diamino-pimelyl amide bond that connects peptidoglycan strands. Likely plays a role in the removal of murein from the sacculus. {ECO:0000255|HAMAP-Rule:MF_01623}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Disulfide bond (3); Metal binding (6); Region (1); Signal peptide (1)
Keywords Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Periplasm;Protease;Signal;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Periplasm {ECO:0000255|HAMAP-Rule:MF_01623}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..19; /evidence=ECO:0000255|HAMAP-Rule:MF_01623
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 30,262
Kinetics
Metal Binding METAL 110; /note=Zinc 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01623; METAL 113; /note=Zinc 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01623; METAL 120; /note=Zinc 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01623; METAL 147; /note=Zinc 2; /evidence=ECO:0000255|HAMAP-Rule:MF_01623; METAL 150; /note=Zinc 2; /evidence=ECO:0000255|HAMAP-Rule:MF_01623; METAL 211; /note=Zinc 1; /evidence=ECO:0000255|HAMAP-Rule:MF_01623
Rhea ID
Cross Reference Brenda